Improved determination method for plasmid copy number using pulsed field gel electrophoresis

BioTechniques

Volumn 28, Issue 1, 2000, Pages 26-30

  Fahnert, Beatrix ^a   Geuther, Rudolf ^a   Hoffmeier, Christine ^a   Roth, Martin ^a  

^a HANS KNÖLL INSTITUTE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA;

ANALYTIC METHOD; ARTICLE; CALCULATION; CHROMOSOME; CHROMOSOME BAND; DNA DEGRADATION; DNA PURIFICATION; NONHUMAN; PLASMID; PULSED FIELD GEL ELECTROPHORESIS; REPRODUCIBILITY;

EID: 0033986532     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00281bm03     Document Type: Article

References (17)

1. Allen, S.P. and H.P. Blaschek. 1990. Plasmid copy number and stability determination in Clostridium perfringens transformants. FEMS Microbiol. Lett. 60:322-327.
2. Boybek, A., T.D. Ray, M.C. Evans and P.J. Dyson. 1998. Novel site-specific DNA modification in Streptomyces: Analysis of preferred intragenic modification sites present in a 5,7 kb amplified DNA sequence. Nucleic Acids Res. 26:3364-3371.
3. Collins, J. and R.H. Pritchard. 1973. Relationship between chromosome replication and F'lac episome replication in Escherichia coli. J. Mol. Biol. 78:143-155.
4. Evans, M. and P. Dyson. 1993. Pulsed-field gel electrophoresis of Streptomyces lividans DNA. Trends Genet. 9:72.
5. Fujimura, H., Y. Sakuma and T. Weimer. 1996. A quantitative method for the determination of plasmid copy number in recombinant yeast. Biologicals 24:67-70.
6. Guerrini, A.M., F. Ascenzioni, T. Gallo and P. Donini. 1993. Determination of plasmid copy number in yeast transformants by means of agarose plugs. Nucleic Acids Res. 21:6047-6048.
7. Ivanov, I.G. and D.R. Bachvarov. 1987. Determination of plasmid copy number by the "boiling" method. Anal. Biochem. 165:137-141.
8. Labes, G., R. Simon and W. Wohlleben. 1990. A rapid method for the analysis of plasmid content and copy number in various streptomycetes grown on agar plates. Nucleic Acids Res. 18:2197.
9. Lee, S.Y., L.D. Mermelstein and E.T. Papoutsakis. 1993. Determination of plasmid copy number and stability in Clostridium acetobutylicum ATCC 824. FEMS Microbiol. Lett. 108:319-323.
10. Muth, G., B. Nußbaumer, W. Wohlleben and A. Pühler. 1989. A vector system with temperature-sensitive replication for gene disruption and mutational cloning. Mol. Gen. Genet. 219:341-348.
11. Neubauer, P. and K. Hofmann. 1990. Verfahren zur Produktion von ColE1-verwandten Plasmiden. Acta Biotechnol. 10:303-305.
12. Nordström, K. 1993. Plasmid Replication and Maintenance, pp. 1-38. In K.G. Hardy (Ed), Plasmids: A Practical Approach. Oxford University Press, New York.
13. Porcher, C., M.C. Malinge, C. Picat and B. Grandchamp. 1992. A simplified method for determination of specific DNA or RNA copy number using quantitative PCR and an automatic DNA sequencer. BioTechniques 13: 106-114.
14. Ray, T., J. Weaden and P. Dyson. 1992. Tris-dependent site-specific cleavage of Streptomyces lividans DNA. FEMS Microbiol. Lett. 75:247-252.
15. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning. CSH Laboratory Press, Cold Spring Harbor, NY.
16. Vazquez-Cruz, C., J.C. Ochoa-Sanchez and G. Olmedo-Alvarez. 1996. Pulse-field gel-eleclrophoretic analysis of the amplification and copy-number stability of an integrational plasmid in Bacillus subtilis. Appl. Microbiol. Biotechnol. 46:55-60.
17. Wrigley-Jones, C., H. Richards, C.R. Thomas and J.M. Ward. 1992. A method for plasmid copy number determination in recombinant Streptomyces. J. Microbiol. Meth. 16:69-80.