Use of recombinatory PCR to insert subtle genetic markers into Moloney murine leukemia virus-based retroviral vectors

Journal of Virological Methods

Volumn 85, Issue 1-2, 2000, Pages 125-136

  Skinner, Cindy R ^a   Jones, Jeffery S ^a  

^a UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

Mutagenic PCR; Recombinatory PCR; Retroviral recombination; Retroviral replication; Retroviral vectors

Indexed keywords

HYGROMYCIN; KANAMYCIN KINASE; NEOMYCIN; VIRUS VECTOR;

ARTICLE; DRUG RESISTANCE; GENETIC MARKER; MOLONEY LEUKEMIA ONCOVIRUS; MUTAGENESIS; MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RETROVIRUS; REVERSE TRANSCRIPTION; VIRUS REPLICATION;

ANIMALS; CELL LINE; CLONING, MOLECULAR; DNA PRIMERS; GENETIC MARKERS; GENETIC VECTORS; KANAMYCIN KINASE; MICE; MOLONEY MURINE LEUKEMIA VIRUS; MUTATION; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; TRANSFECTION;

MOLONEY MURINE LEUKEMIA VIRUS; MURINAE;

EID: 0033985552     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(99)00159-7     Document Type: Article

References (23)

1. Anderson J.A., Bowman E.H., Hu W.S. Retroviral recombination rates do not increase linearly with marker distance and are not limited by the size of the recombining subpopulation. J. Virol. 72(2):1998;1195-1202.
2. Bolshakov, S.B., Freed, D.D., Su, J.R., Jones, J.S., 1999. Alteration of the U5 inverted repeat of spleen necrosis virus affects strand transfers during reverse transcription. J. Virol. (submitted).
3. Coffin J.M. Retroviridae: The viruses and their replication. Fields B.N., Knipe D.M., Howley P.M., Chanock R.M., Melnick J.L., Monath T.P., Roizman B., Straus S.E. 3rd edn Fields Virology. 2:1996;1767-1847 Lippincott-Raven, Philadelphia, PA.
4. Dougherty J.P., Temin H.M. High mutation rate of a spleen necrosis virus-based retrovirus vector. Mol. Cell. Biol. 6:1986;4387-4395.
5. Ho S.N., Hunt H.D., Horton R.M., Pullen J.K., Pease L.R. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 77:1989;51-59.
6. Horton R.M., Cai Z., Ho S.N., Pease L.R. Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. Biotechniques. 8:1990;528-535.
7. Hu W.-S., Temin H.M. Genetic consequences of packaging two RNA genomes in one retroviral particle: pseudodiploidy and high rate of genetic recombination. Proc. Natl. Acad. Sci. USA. 87:1990a;1556-1560.
8. Hu W.-S., Temin H.M. Retroviral recombination and reverse transcription. Science. 250:1990b;1227-1233.
9. Hu W.-S., Temin H.M. Effect of gamma radiation on retroviral recombination. J. Virol. 66:1992;4457-4463.
10. Ito W., Ishiguro H., Kurosawa Y. A general method for introducing a series of mutations into cloned DNA using the polymerase chain reaction. Gene. 102:1991;67-70.
11. Jones J.S., Allan R.W., Temin H.M. Alteration of location of dimer linkage sequence in retroviral RNA: little effect on replication or homologous recombination. J. Virol. 67:1993;3151-3158.
12. Jones J.S., Allan R.W., Seufzer B., Temin H.M. Co-packaging of differently sized retroviral genomic RNAs: little effect on retroviral replication or recombination. J. Virol. 68:1994a;4097-4103.
13. Jones J.S., Allan R.W., Temin H.M. One retroviral RNA is sufficient for synthesis of viral DNA. J. Virol. 68(1):1994b;207-216.
14. Luciw P.A., Leung N.J. Mechanisms of retrovirus replication. Levy J.A. The Retroviridae. The Viruses. 1:1992;159-298 Plenum Press, New York.
15. Miller A.D., Buttimore C. Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production. Mol. Cell Biol. 6:1986;2895-2902.
16. Miller A.D., Rosman G.J. Improved retroviral vectors for gene transfer and expression. BioTechniques. 7:1989;980-990.
17. Miller A.D., Garcia J.V., von Suhr N., Lynch C.M., Wilson C., Eiden M.V. Construction and properties of retrovirus packaging cells based on Gibbon ape leukemia virus. J. Virol. 65:1991;2220-2224.
18. Panganiban A.T., Fiore D. Ordered interstrand and intrastrand DNA transfer during reverse transcription. Science. 241:1988;1064-1069.
19. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
20. Skalka A.M., Goff S.P. Reverse Transcriptase. 1993;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
21. Stuhlman H., Berg P. Homologous recombination of copackaged retrovirus RNAs during reverse transcription. J. Virol. 66:1992;2378-2388.
22. Yang S., Temin H.M. A double hairpin structure is necessary for the efficient encapsidation of spleen necrosis virus. EMBO J. 13:1994;713-726.
23. Yin P.D., Pathak V.P., Rowan A.E., Teufel R.J., Hu W.S. Utilization of nonhomologous minus-strand DNA transfer to generate recombinant retroviruses. J. Virol. 71:1997;2487-2494.