Isolation and characterization of cholic acid 7α-dehydroxylating fecal bacteria from cholesterol gallstone patients

Journal of Hepatology

Volumn 32, Issue 1, 2000, Pages 4-10

  Wells, James E ^a   Berr, Frieder ^b   Thomas, Linzi A ^c   Dowling, R Hermon ^d   Hylemon, Phillip B ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF LEIPZIG   (Germany)

^c MORRISTON HOSPITAL   (United Kingdom)

^d GUY S HOSPITAL   (United Kingdom)

Author keywords

Cholesterol gallstones; Clostridium; Deoxycholic acid; Intestinal microflora

Indexed keywords

CHOLESTEROL; CHOLIC ACID; DEOXYCHOLIC ACID;

ADULT; AGED; ARTICLE; BACTERIUM ISOLATION; CHOLESTEROL STONE; CLINICAL ARTICLE; CLOSTRIDIUM; CONTROLLED STUDY; FECES; FEMALE; HUMAN; HYDROXYLATION; MALE; PRIORITY JOURNAL; THIN LAYER CHROMATOGRAPHY;

EID: 0033985517     PISSN: 01688278     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-8278(00)80183-X     Document Type: Article

References (22)

1. Vlahcevic ZR, Heuman DM, Hylemon PB. Physiology and pathophysiology of enterohepatic circulation of bile acids. Zakim D, Boyer TD. Hepatology: a Textbook of Liver Disease. 1996;376-401 W.B. Saunders, Philadelphia.
2. Baron SF, Hylemon PB. Biotransformation of bile acids, cholesterol, and steroid hormones. Mackie RI, White BA. Gastrointestinal Microbiology, volume 1. 1996;470-510 Chapman and Hall, New York.
3. Berr F, Kullak-Ublick GA, Paumgartner G, Münzing W, Hylemon PB. Increased bacterial 7α-dehydroxylation as a mechanism for increasing deoxycholic acid input and cholesterol saturation of bile in patients with gallstones. Gastroenterology. 111:1996;1611-1620.
4. Hussaini SH, Pereira SP, Veysey MJ, Kennedy C, Jenkins P, Murphy GM, et al. Role of gall bladder emptying and intestinal transit in the pathogenesis of octreatide induced gall bladder stones. Gut. 35:1996;775-783.
5. Dowling RH, Veysey MJ, Pereira SP, Hussaini SH, Thomas LA, Wass JA, et al. Role of intestinal transit in the pathogenesis of gallbladder stones. Can J Gastroenterol. 11:1997;57-64.
6. Lewis SJ, Heaton KW. Increase butyrate concentration in the distal colon by accelerating intestinal transit. Gut. 41:1997;245-251.
7. Low-Beer TS, Nutter S. Colonic bacterial activity, biliary cholesterol saturation, and pathogenesis of gallstones. Lancet. ii:1978;1063-1065.
8. Marcus SN, Heaton KW. Intestinal transit, deoxycholic acid and the cholesterol saturation of bile: three interrelated factors. Gut. 27:1986;550-558.
9. Shoda J, He B-F, Tanaka N, Matsuzaki I, Osuga T, Yamamori S, et al. Increase of deoxycholate in supersaturated bile of patients with cholesterol gallstone disease and its correlation with de novo synthesis of cholesterol and bile acids in the liver, gallbladder emptying, and small intestine transit. Hepatology. 21:1995;1291-1302.
10. Thomas LA, Bathgate T, Veysey MJ, Smeeton N, French GL, Murphy GM, et al. Transit-induced changes in colonic bacteriology, bile acid metabolizing enzymes and luminal pH influence deoxycholic acid formation. Paumgartner G, Stiehl A, Keppler D, Gerok W, Leuschner U. Bile Acids and Cholestatis. 1999;229-237 Kluwer Academic Publishers, Falk Symposium 108.
11. Gustafsson BE, Midvedt T, Norman A. Isolated fecal microorganisms capable of 7α-dehydroxylating bile acids. J Exp Med. 123:1966;413-432.
12. Hayakawa S, Hattori T. 7α-Dehydroxylation of cholic acid by Clostridium bifermentans strain ATCC 9714 and Clostridium sordelii strain NCIB. FEBS Lett. 6:1970;131-133.
13. Stellwag EJ, Hylemon PB. 7α-Dehydroxylation of cholic acid and chenodeoxycholic acid by Clostridium leptum. J Lipid Res. 20:1979;325-333.
14. Hirano S, Nakama R, Tamaki M, Mauda N, Oda H. Isolation and characterization of thirteen intestinal microorganisms capable of 7α-dehydroxylation of bile acids. Appl Environ Microbiol. 41:1981;737-745.
15. Takamine F, Imamura T. Isolation and characterization of bile acid-inducible 7α-dehydroxylating bacteria from human feces. Microbiol Immunol. 39:1995;11-18.
16. Doerner KC, Takamine F, LaVoie CP, Mallonee DH, Hylemon PB. Assessment of fecal bacteria with bile acid 7α-dehydroxylating activity for the presence of bai-like genes. Appl Environ Microbiol. 63:1997;1185-1188.
17. Eneroth P. Thin layer chromatography of bile acids. J Lipid Res. 4:1963;11-16.
18. Holdeman LV, Moore WEC. Anaerobe Laboratory Manual, 2nd ed. 1972;VPI Anaerobe Laboratory, Blacksburg, VA.
19. Marmur J. A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol. 3:1961;208-218.
20. Gopal-Srivastava R, Mallonee DH, White WB, Hylemon PB. Multiple copies of a bile acid-inducible gene in Eubacterium sp. strain VPI 12708. J Bacteriol. 172:1990;4420-4426.
21. Koch AL. Growth measurement. Costilow RN. Manual of Methods for General Bacteriology. 1981;179-207 Am Soc Microbiol, Washington, D.C.
22. Sokal RR, Rolf FJ. Biometry, the Principles and Practice of Statistics in Biological Research. 1969;WH Freeman, San Francisco.