Technical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay

International Journal of Radiation Biology

Volumn 76, Issue 1, 2000, Pages 61-65

  Ward, T H ^a   Marples, B ^b  

^a CHRISTIE HOSPITAL   (United Kingdom)

^b MOUNT VERNON HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; FLUOROCHROME;

ARTICLE; CARCINOMA CELL; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; HUMAN; HUMAN CELL; IMAGE ANALYSIS; PRIORITY JOURNAL; RADIATION DOSE; RADIATION INJURY;

EID: 0033985066     PISSN: 09553002     EISSN: None     Source Type: Journal    
DOI: 10.1080/095530000139014     Document Type: Article

References (7)

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2. Fairbairn, D. W., Olive, P. L. and O'Neill, K. L., 1995, The comet assay: a comprehensive review. Mutation Research, 339, 37-59.
3. Marples, B., Longhurst, D., Eastham, A. M. and West, C. M. L., 1998, The ratio of initial/residual DNA damage predicts intrinsic radiosensitivity in 7 cervix carcinoma cell lines. British Journal of Cancer, 77, 1108-1114.
4. Olive, P. L., Wlodek, D. and Banath, J. P., 1991, DNA double strand breaks measured in individual cells subjected to gel electrophoresis. Cancer Research, 51, 4671-4676.
5. Olive, P. L., Wlodek, D., Durand, R. E. and Banath, J. P., 1992, Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Experimental Cell Research, 198, 259-267.
6. Singh, N. P., Stephens, R. E. and Schneider, E. L., 1994, Modifications to alkaline microgel electrophoresis for sensitive detection of DNA damage. International Journal of Radiation Biology, 66, 23-28.
7. Ward, T. H., Butler, J., Shahbakhti, H. and Richards, J. T., 1997, Comet assay studies on the activation of two diaziridinylbenzoquinones in K562 cells. Biochemical. Pharmacology, 53, 1115-1121.