High-density hapten labeling and HRP conjugation of oligonucleotides for use as in situ hybridization probes to detect mRNA targets in cells and tissues

Journal of Histochemistry and Cytochemistry

Volumn 48, Issue 1, 2000, Pages 133-145

  Luehrsen, Kenneth R ^a   Davidson, Scott ^c   Lee, Yun Ji ^c   Rouhani, Riaz ^b   Soleimani, Ali ^c   Raich, Teresa ^c   Cain, Carol A ^c   Collarini, Ellen J ^c   Yamanishi, Douglas T ^c   Pearson, Jennifer ^c   Magee, Kerry ^c   Madlansacay, Mary Rose ^c   Bodepudi, Veeraiah ^c   Davoudzadeh, David ^c   Schueler, Paula A ^c   Mahoney, Walt ^c  

^a Roche Diagnostics   (United States)

^b MicroGenics DRI   (United States)

^c NONE

Author keywords

Branching phosphoramidite; Chorionic villus; Fetal hemoglobin; In situ hybridization; Nucleated red blood cell; Oligonucleotide probe; Tyramide signal amplification

Indexed keywords

BIOTIN; DIGOXIGENIN; FLUORESCEIN; HAPTEN; HORSERADISH PEROXIDASE; MESSENGER RNA;

ARTICLE; HUMAN; HUMAN CELL; HUMAN TISSUE; IN SITU HYBRIDIZATION; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; SIGNAL PROCESSING; SYNTHESIS;

EID: 0033985057     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540004800114     Document Type: Article

References (32)

1. Adler K, Erickson T, Bobrow M (1997) High sensitivity detection of HPV-16 in SiHa and CaSki cells using FISH enhanced TSA. Histochem Cell Biol 108:321-324
2. Autillo-Touati A, Joannes M, d'Ercole C, Robaglia-Schlupp A, Lambert A, Mazzella E, Blanc B, Seite R (1998) HPV typing by in situ hybridization on cervical cytologic smears with ASCUS. Acta Cytol 42:631-638
3. Chumbley G, King A, Holmes N, Loke YW (1993) In situ hybridization and Northern blot demonstration of HLA-G mRNA in human trophoblast populations by locus-specific oligonucleotide. Hum Immunol 37:17-22
4. Eiben B, Trawicki W, Hammans W, Goebel R, Epplen JT (1998) A prospective comparative study on fluorescence in situ hybridization (FISH) of uncultured amniocytes and standard karyotype analysis. Prenat Diagn 18:901-906
5. Farquharson MA, Harvie R, Kennedy A, McNicol AM (1992) Detection of mRNA by in situ hybridisation and in Northern blot analysis using oligodeoxynucleotide probes labelled with alkaline phosphatase. J Clin Pathol 45:999-1002
6. Forster AC, McInnes JL, Skingle DC, Symons RH (1985) Nonradioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin. Nucleic Acids Res 13:745-761
7. Harper SJ, Bailey E, McKeen CM, Stewart AS, Pringle JH, Feehally J, Brown T (1997) A comparative study of digoxigenin, 2,4-dinitrophenyl, and alkaline phosphatase as deoxyoligonucleotide labels in non-radioisotopic in situ hybridisation. J Clin Pathol 50:686-690
8. Hauptmann G, Gerster T (1994) Two-color whole-mount in situ hybridization to vertebrate and Drosophila embryos. Trends Genet 10:266
9. Horn T, Warner BD, Running JA, Downing K, Clyne J, Urdea MS (1989) The synthesis of branched oligonucleotides as signal amplification multimers for use in nucleic acid assays. Nucleosides Nucleotides 8:875-877
10. Hoshina M, Hussa R, Pattillo R, Camel H M, Boime I (1984) The role of trophoblast differentiation in the control of the hCG and hPL genes. Adv Exp Med Biol 176:299-312
11. Hougaard DM, Hansen H, Larsson L-I (1997) Non-radioactive in situ hybridization for mRNA with emphasis on the use of oligodeoxynucleotide probes. Histochem Cell Biol 108:335-344
12. Huang CC, Qiu JT, Kashima ML, Kurman RJ, Wu TC (1998) Generation of type-specific probes for the detection of single-copy human papillomavirus by a novel in situ hybridization method. Mod Pathol 11:971-977
13. Jablonski E, Moomaw EW, Tullis RH, Ruth JL (1986) Preparation of oligo-deoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes. Nucleic Acids Res 14:6115-6129
14. Jalal SM, Law ME, Carlson RO, Dewald GW (1998) Prenatal detection of aneuploidy by directly labeled multicolored probes and interphase fluorescence in situ hybridization. Mayo Clin Proc 73:132-137
15. Jowett T, Lettice L (1994) Whole-mount in situ hybridization on zebrafish embryos using a mixture of dogoxigenin and fluoresceinlabeled probes. Trends Genet 10:73-74
16. Larsson L-I, Hougaard DM (1993) Sensitive detection of rat gastrin mRNA by in situ hybridization with chemically biotinylated oligodeoxynucleotides: validation, quantitation, and double-staining studies. J Histochem Cytochem 41:157-163
17. Luo HY, Liang XL, Frye C, Wonio M, Hankins GDV, Chui DHK, Alter BP (1999) Embryonic hemoglobins are expressed in definitive cells. Blood 94:359-361
18. Mesker WE, Ouwerkerk-van Velzen MC, Oosterwijk JC, Bernini LF, Golbus MS, Kanhai HH, Van Ommen GJ, Tanke HJ (1998) Two-colour immunocytochemical staining of gamma (γ) and epsilon (ε) type haemoglobin in fetal red cells. Prenat Diagn 18:1131-1137
19. Nath J, Johnson KL (1997) Fluorescence in situ hybridization (FISH): DNA probe production and hybridization criteria. Biotech Histochem 73:6-22
20. Panoskaltsis-Mortari A, Bucy RP (1995) In situ hybridization with digoxigenin-labeled RNA probes: facts and artifacts. Biotechniques 18:300-307
21. Raap AK, van de Rijke FM, Dirks RW (1994) mRNA in situ hybridization to in vitro cultured cells. Methods Mol Biol 33:293-300
22. Ruth JL (1994) Oligonucleotide-enzyme conjugates. Methods Mol Biol 26:167-185
23. Sakbun V, Ali SM, Lee YA, Jara CS, Bryant-Greenwood GD (1990) Immunocytochemical localization and messenger ribonucleic acid concentrations for human placental lactogen in amnion, chorion, decidua, and placenta. Am J Obstet Gynecol 162:1310-1317
24. Schmidt BF, Chao J, Zhu Z, DeBiasio RL, Fisher G (1997) Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J Histochem Cytochem 45: 365-373
25. Speel EJM, Ramaekers FCS, Hopman AHN (1995) Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry. 'Who is still afraid of red, green and blue?' Histochem J 7:833-858
26. Sumiyoshi Y, Shirakusa T, Yamashita Y, Maekawa T, Hideshima T, Sakai T, Kawahara K, Kikuchi M (1998) Detection of chromogranin A mRNA in small cell lung carcinoma using a new, highly sensitive in situ hybridization method with a non-radioisotope oligonucleotide probe. Cancer 82:468-473
27. Taneja KL, Singer RH (1990) Detection and localization of actin mRNA isoforms in chicken muscle cells by in situ hybridization using hiotinated oligonucleotide probes. J Cell Biochem 44:241-252
28. van de Corput MP, Dirks RW, van Gijlswijk RP, van Binnendijk E, Hattinger CM, de Paus RA, Landegent JE, Raap AK (1998a) Sensitive mRNA detection by fluorescence in situ hybridization using horseradish peroxidase-labeled oligodeoxynucleotides and tyramide signal amplification. J Histochem Cytochem 46:1249-1259
29. van de Corput MP, Dirks RW, van Gijlswijk RP, van de Rijkc FM, Raap AK (1998b) Fluorescence in situ hybridization using horse-radish peroxidase-labeled oligodeoxynucleotides and tyramide signal amplification for sensitive DNA and mRNA detection. Histochem Cell Biol 110:431-437
30. van Gijlswijk RP, Wiegant J, Vervenne R, Lasan R, Tanke HJ, Raap AK (1996) Horseradish peroxidase-labeled oligonucleotides and fluorescent tyramides for rapid detection of chromosome-specific repeat sequences. Cytogenet Cell Genet 75:258-262
31. Wide M, Persson H, Gunnarsson S, Wide L, Seifi L (1989) High frequency of in situ hybridization on thin plastic sections of human placenta with a cDNA probe for beta HCG. J Histochem Cytochem 37:1193-1196
32. Wide M, Persson H, Lundkvist O, Wide L (1988) Localization of the mRNA for the beta-subunit of placental hCG by in situ hybridization. Acta Endocrinol 119:69-74