Molecular cloning and characterization of the 120-kilodalton protein gene of Ehrlichia canis and application of the recombinant 120-kilodalton protein for serodiagnosis of canine ehrlichiosis

Journal of Clinical Microbiology

Volumn 38, Issue 1, 2000, Pages 369-374

  Yu, Xue Jie ^a   McBride, Jere W ^a   Diaz, C Marcela ^a   Walker, David H ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN; OUTER MEMBRANE PROTEIN; PROTEIN P120;

ARTICLE; DNA SEQUENCE; EHRLICHIA CANIS; GENE DELETION; IMMUNOBLOTTING; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SERODIAGNOSIS; TANDEM REPEAT;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DOG DISEASES; DOGS; EHRLICHIA; EHRLICHIA CHAFFEENSIS; EHRLICHIOSIS; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; REPETITIVE SEQUENCES, AMINO ACID; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SEROLOGIC TESTS; SPECIES SPECIFICITY;

EID: 0033984293     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Allred, D. R., T. C. McGuire, G. H. Palmer, S. R. Leib, T. M. Harkins, T. F. McElwain, and A. F. Barbet. 1990. Molecular basis for surface antigen size polymorphisms and conservation of a neutralization-sensitive epitope in Anaplasma marginale. Proc. Natl. Acad. Sci. USA 87:3220-3224.
2. Anderson, B. E., J. E. Dawson, D. C. Jones, and K. H. Wilson. 1991. Ehrlichia chaffeensis, a new species associated with human ehrlichiosis. J. Clin. Microbiol. 29:2838-2842.
3. Anderson, B. E., C. E. Greene, D. C. Jones, and J. E. Dawson. 1992. Ehrlichia ewingii sp. nov., the etiologic agent of canine granulocytic ehrlichiosis. Int. J. Syst. Bacteriol. 42:299-302.
4. Bakken, J. S., J. S. Dumler, S. M. Chen, M. R. Eckman, L. L. Van Etta, and D. H. Walker. 1994: Human granulocytic ehrlichiosis in the upper Midwest United States. A new species emerging? JAMA 272:212-218.
5. Buhles, W. C., Jr., D. L. Huxsoll, and M. Ristic. 1974. Tropical canine pancytopenia: clinical, hematologic, and serologic response of dogs to Ehrlichia canis infection, tetracycline therapy, and challenge inoculation. J. Infect. Dis. 130:357-367.
6. Chen, S. M., J. S. Dumler, J. S. Bakken, and D. H. Walker. 1994. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J. Clin. Microbiol. 32:589-595.
7. Chen, S. M., J. S. Dumler, H. M. Feng, and D. H. Walker. 1994. Identification of the antigenic constituents of Ehrlichia chaffeensis. Am. J. Trop. Med. Hyg. 50:52-58.
8. Chen, S. M., X. J. Yu, V. L. Popov, E. L. Westerman, F. G. Hamilton, and D. H. Walker. 1997. Genetic and antigenic diversity of Ehrlichia chaffeensis: comparative analysis of a novel human strain from Oklahoma and previously isolated strains. J. Infect. Dis. 175:856-863.
9. Dawson, J. E., Y. Rikihisa, S. A. Ewing, and D. B. Fishbein. 1991. Serologic diagnosis of human ehrlichiosis using two Ehrlichia canis isolates. J. Infect. Dis. 163:564-567.
10. Fishbein, D. B., L. A. Sawyer, C. J. Holland, E. B. Hayes, W. Okoroanyanwu, D. Williams, K. Sikes, M. Ristic, and J. E. McDade. 1987. Unexplained febrile illnesses after exposure to ticks. Infection with an Ehrlichia? JAMA 257:3100-3104.
11. Greene, C. E., and J. W. Harvey. 1984. Canine ehrlichiosis, p. 545-561. In C. E. Greene (ed), Clinical microbiology and infectious diseases of the dog and cat. The W. B. Saunders Co., Philadelphia, Pa.
12. Groves, M. G., G. L. Dennis, H. L. Amyx, and D. L. Huxsoll. 1975. Transmission of Ehrlichia canis to dogs by ticks (Rhipicephalus sanguineus). Am. J. Vet. Res. 34:937-940.
13. Hollingshead, S. K., V. A. Fischetti, and J. R. Scott. 1986. Complete nucleotide sequence of type 6 M protein of the group A Streptococcus. Repetitive structure and membrane anchor. J. Biol. Chem. 261:1677-1686.
14. Kemp, D. J., R. L. Coppel, and R. F. Anders. 1987. Repetitive proteins and genes of malaria. Annu. Rev. Microbiol. 41:181-208.
15. Klein, P., M. Kanehisa, and C. DeLisi. 1985. The detection and classification of membrane-spanning proteins. Biochim. Biophys. Acta 815:468-476.
16. Lewis, G. E., Jr., M. Ristic, R. D. Smith, T. Lincoln, and E. H. Stephenson. 1977. The brown dog tick Rhipicephalus sanguineus and the dog as experimental hosts of Ehrlichia canis. Am. J. Vet. Res. 38:1953-1955.
17. Maeda, K., N. Markowitz, R. C. Hawley, M. Ristic, D. Cox, and J. E. McDade. 1987. Human infection with Ehrlichia canis, a leukocytic rickettsia. N. Engl. J. Med. 316:853-856.
18. McBride, J. W., X. Yu, and D. H. Walker. Glycosylation of homologous immunodominant proteins of Ehrlichia chaffeensis and Ehrlichia canis. Infect. Immun., in press.
19. McGeoch, D. J. 1985. On the predictive recognition of signal peptide sequences. Virus Res. 3:271-286.
20. Perez, M., Y. Rikihisa, and B. Wen. 1996. Ehrlichia canis-like agent isolated from a man in Venezuela: antigenic and genetic characterization. J. Clin. Microbiol. 34:2133-2139.
21. Popov, V. L., X. Yu, and D. H. Walker. The 120 kDa outer membrane protein of Ehrlichia chaffeensis: preferential expression of dense-core cells and gene expression in Escherichia coli associated with attachment and entry. Microb. Pathog., in press.
22. Signas, C., G. Raucci, K. Jonsson, P. E. Lindgren, G. M. Anantharamaiah, M. Hook, and M. Lindberg. 1989. Nucleotide sequence of the gene for a fibronectin-binding protein from Staphylococcus aureus: use of this peptide sequence in the synthesis of biologically active peptides. Proc. Natl. Acad. Sci. USA 86:699-703.
23. Storey, J. R., L. A. Doros-Richert, C. Gingrich-Baker, K. Munroe, T. N. Mather, R. T. Coughlin, G. A. Beltz, and C. I. Murphy. 1998. Molecular cloning and sequencing of three granulocytic Ehrlichia genes encoding high-molecular-weight immunoreactive proteins. Infect. Immun. 66:1356-1363.
24. von Heijne, G. 1986. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 14:4683-4690.
25. Walker, J. S., J. D. Rundquist, R. Taylor, B. L. Wilson, M. R. Andrews, J. Barck, A. L. Hogge, Jr., D. L. Huxsoll, P. K. Hildebrandt, and R. M. Nims. 1970. Clinical and clinicopathologic findings in tropical canine pancytopenia. J. Am. Vet. Med. Assoc. 157:43-55.
26. Weiss, E., J. C. Coolbaugh, and J. C. Williams. 1975. Separation of viable Rickettsia typhi from yolk sac and L cell host components by renografin density gradient centrifugation. Appl. Microbiol. 30:456-463.
27. Yu, X.-J., P. A. Crocquet-Valdes, and D. H. Walker. 1996. Cloning and sequencing of the gene for a 120-kDa immunodominant protein of Ehrlichia chaffeensis. Gene 184:149-154.
28. Yu, X.-J., and D. H. Walker. 1997. Sequence and characterization of an Ehrlichia chaffeensis gene encoding 314 amino acids highly homologous to the NAD A enzyme. FEMS Microbiol. Lett. 154:53-58.
29. Yu, X.-J., P. A. Crocquet-Valdes, L. C. Cullman, and D. H. Walker. 1996. The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool. J. Clin. Microbiol. 34:2853-2855.