Routine detection and quantification of hepatitis B virus DNA in clinical laboratories: Performance of three commercial assays

Journal of Virological Methods

Volumn 85, Issue 1-2, 2000, Pages 11-21

  Pawlotsky, Jean Michel ^a,b   Bastie, Anne ^a   Hézode, Christophe ^a   Lonjon, Isabelle ^a   Darthuy, Françoise ^a   Rémiré, Jocelyne ^a   Dhumeaux, Daniel ^a,b  

^a UNIVERSITÉ PARIS OUEST NANTERRE LA DÉFENSE   (France)

^b INSERM   (France)

Author keywords

Branched DNA assay; Hepatitis B virus; Liquid phase hybridization; Polymerase chain reaction; Viral load

Indexed keywords

VIRUS DNA;

ARTICLE; ASSAY; BIOASSAY; CONTROLLED STUDY; DNA DETERMINATION; HEPATITIS B; HEPATITIS B VIRUS; HUMAN; HYBRIDIZATION; MONITORING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; STANDARDIZATION; VIRUS DETECTION; VIRUS LOAD;

DATA INTERPRETATION, STATISTICAL; DNA, VIRAL; HEPATITIS B; HEPATITIS B VIRUS; HUMANS; LABORATORIES, HOSPITAL; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

HEPATITIS B VIRUS; MUREX;

EID: 0033983436     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(99)00149-4     Document Type: Article

References (21)

1. Carman W.F., Jacyna M.R., Hadziyannis S. et al. Mutation preventing formation of hepatitis B e antigen in patients with chronic hepatitis B infection. Lancet. 2:1989;588-591.
2. Gerken G., Gomes J., Lampertico P. et al. Clinical evaluation and applications of the Amplicor HBV Monitor test, a quantitative HBV DNA PCR assay. J. Virol. Methods. 74:1998;155-165.
3. Gibson U.E.M., Heid C.A., Williams P.M. A novel method for real time quantitative RT-PCR. Genome Res. 6:1996;995-1001.
4. Hawkins A., Davidson F., Simmonds P. Comparison of plasma virus loads among individuals infected with hepatitis C virus (HCV) genotypes 1, 2, and 3 by Quantiplex HCV RNA assay versions 1 and 2, Roche Monitor assay, and in-house limiting dilution method. J. Clin. Microbiol. 35:1997;187-192.
5. Hendricks D.A., Stowe B.J., Hoo B.S. et al. Quantitation of HBV DNA in human serum using a branched DNA (bDNA) signal amplification assay. Am J Clin. Pathol. 104:1995;537-546.
6. Hoofnagle J.H., Shafritz D.A., Popper H. Chronic type B hepatitis and the 'healthy' HBsAg carrier state. Hepatology. 7:1987;758-763.
7. Kaneko S., Miller R.H., Di Bisceglie A.M., Feinstone S.M., Hoofnagle J.H., Purcell R.H. Detection of hepatitis B virus DNA in serum by polymerase chain reaction: application for clinical diagnosis. Gastroenterology. 99:1990;799-804.
8. Kessler H.H., Pierer K., Santner B.I. et al. Quantitative detection of hepatitis B virus DNA with a new PCR assay. Clin. Chem. Lab. Med. 36:1998;601-604.
9. Kuhns M.C., McNamara A.L., Perrillo R.P., Cabal C.M., Campbell C.R. Quantitation of hepatitis B viral DNA by solution hybridization: comparison with DNA polymerase and hepatitis B e antigen during antiviral therapy. J. Med. Virol. 27:1989;274-281.
10. Lau J.Y.N., Wright T.L. Molecular virology and pathogenesis of hepatitis B. Lancet. 342:1993;1335-1340.
11. Noborg U., Gusdal A., Pisa E.K., Hedrum A., Lindh M. Automated quantitative analysis of hepatitis B virus DNA by using the Cobas Amplicor HBV Monitor test. J. Clin. Microbiol. 37:1999;2793-2797.
12. Pawlotsky J.M. Measuring hepatitis C viremia in clinical samples: can we trust the assays? Hepatology. 26:1997;1-4.
13. Pawlotsky J.M., Bastie A., Lonjon I. et al. What technique should be used for routine detection and quantification of HBV DNA in clinical samples? J. Virol. Methods. 65:1997;245-253.
14. Perrillo R.P., Schiff E.R., Davis G.L. et al. A randomized, controlled trial of interferon alfa-2b alone and after prednisone withdrawal for the treatment of chronic hepatitis B. N. Engl. J. Med. 321:1990;295-301.
15. Ranki M., Schatzl H.M., Zachoval R., Uusi-Oukari M., Lehtovaara P. Quantification of hepatitis B virus DNA over a wide range from serum for studying viral replicative activity in response to treatment and in recurrent infection. Hepatology. 21:1995;1492-1499.
16. Urdea M.S., Horn T., Fultz T.J. et al. Branched DNA amplification multimers for the sensitive, direct detection of human hepatitis viruses. Nucleic Acids Res. Symp. Ser. 24:1991;197-200.
17. Wright T.L., Lau J.Y.N. Clinical aspects of hepatitis B virus infection. Lancet. 342:1993;1340-1344.
18. Yen-Lieberman B., Brambilla D., Jackson B. et al. Evaluation of a quality assurance program for quantitation of human immunodeficiency virus type 1 RNA in plasma by the AIDS clinical trials group virology laboratories. J. Clin. Microbiol. 34:1996;2695-2701.
19. Zarski J.P., Kuhns M., Berck L. et al. Comparison of a quantitative standardized HBV DNA assay and a classical spot hybridization test in chronic active hepatitis B patients undergoing antiviral therapy. Res. Virol. 140:1989;2830-2831.
20. Zoulim F., Mimms L., Floreani M. et al. New assays for quantitative determination of viral markers in management of chronic hepatitis B virus infection. J. Clin. Microbiol. 30:1992;1111-1119.
21. Zoulim F., Trépo C. Drug therapy for chronic hepatitis B: antiviral efficacy and influence of hepatitis B virus polymerase mutations on the outcome of therapy. J. Hepatol. 29:1998;151-168.