Purification of the membrane binding domain of cytochrome b5 by immobilised nickel chelate chromatography

Journal of Chromatography B: Biomedical Sciences and Applications

Volumn 737, Issue 1-2, 2000, Pages 119-130

  Begum, Rukhsana R ^a   Newbold, Richard J ^a   Whitford, David ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Cytochrome b5; Membrane binding domain; Nickel chelates; Purification; Thioredoxin

Indexed keywords

CYTOCHROME B5; HYBRID PROTEIN; MEMBRANE PROTEIN; THIOREDOXIN;

AFFINITY CHROMATOGRAPHY; CONFERENCE PAPER; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENE FUSION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TECHNIQUE;

BASE SEQUENCE; CELL MEMBRANE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROMES B5; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HYDROLYSIS; NICKEL; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; TRYPSIN;

EID: 0033982799     PISSN: 13872273     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-4347(99)00480-6     Document Type: Article

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