Survival of Bifidobacterium longum immobilized in calcium alginate beads in simulated gastric juices and bile salt solution

Applied and Environmental Microbiology

Volumn 66, Issue 2, 2000, Pages 869-873

  Lee, Ki Yong ^a   Heo, Tae Ryeon ^a  

^a Inha University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

BILE SALT; CALCIUM ALGINATE;

ARTICLE; BIFIDOBACTERIUM LONGUM; CELL COUNT; IMMOBILIZED CELL; MATHEMATICAL MODEL; MICROENCAPSULATION; NONHUMAN; PARTICLE SIZE; SIMULATION; STOMACH JUICE; SURVIVAL RATE;

ALGINATES; BIFIDOBACTERIUM; BILE ACIDS AND SALTS; CELLS, IMMOBILIZED; COLONY COUNT, MICROBIAL; CULTURE MEDIA; GASTRIC JUICE; GLUCURONIC ACID; HEXURONIC ACIDS; MICROSPHERES; MODELS, BIOLOGICAL;

ACTINOBACTERIA (CLASS); BIFIDOBACTERIUM LONGUM; UNCULTURED ACTINOMYCETE;

EID: 0033981046     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.66.2.869-873.2000     Document Type: Article

References (23)

1. Audet, P., C. Paquin, and C. Lacroix. 1988. Immobilized growing lactic acid bacteria with κ-carrageenan-locust bean gum gel. Appl. Microbiol. Biotechnol. 29:11-18.
2. Berrada, N., J. F. Lemelan, G. Laroche, P. Thouvenot, and M. Piaia. 1991. Bifidobacterium from fermented milks: survival during gastric transit. J. Dairy Sci. 74:409-413.
3. Camelin, I., C. Lacroix, C. Paquin, H. Prevost, R. Cachon, and C. Divies. 1993. Effect of chelatants on gellan gel rheological properties and setting temperature for immobilization of living bifidobacteria. Biotechnol. Prog. 9:291-297.
4. Champagne, C. P., C. Gaudy, D. Poncelet, and R. J. Neufeld. 1992. Lactococcus lactis release from calcium alginate beads. Appl. Environ. Microbiol. 58:1429-1434.
5. Conway, P. L., S. L. Gorbach, and B. R. Goldin. 1987. Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. J. Dairy Sci. 70:1-12.
6. Gopal, A., N. P. Shah, and J. Roginski. 1996. Bile tolerance, taurocholate deconjugation and cholesterol removal by Lactobacillus acidophilus and Bifidobacterium spp. Milchwissenschaft 51:519-623.
7. Hannoun, B., and G. Stephanupoulos. 1986. Diffusion coefficients of glucose and ethanol in cell-free and cell-occupied calcium alginate membranes. Biotechnol. Bioeng. 28:829-835.
8. Ibrahim, S. A., and A. Bezkorovainy. 1993. Survival of bifidobacteria in the presence of bile salt. J. Sci. Food Agric. 62:351-354.
9. King, G., A. Daugulis, M. Goosen, P. Faulkner, and D. Bayly. 1989. Alginate concentration: a key factor in growth of temperature-sensitive baculovirus-infected cells in microcapsules. Biotechnol. Bioeng. 34:1085-1091.
10. Klaver, F. A. M., and R. Meer. 1993. The assumed assimilation of cholesterol by lactobacilli and Bifidobacterium bifidum is due to their bile salt-deconjugating activity. Appl. Environ. Microbiol. 59:1120-1124.
11. Klein, J., and D. Vorlop. 1985. Immobilization technique - cells, p. 203-224. In M. Moo-Young (ed.), Comprehensive biotechnology, vol. 2. Pergamon Press, Oxford, United Kingdom.
12. Kolot, F. B. 1984. Immobilized cells for solvent production. Process Biochem. 19:7-13.
13. Lacroix, C., C. Paquin, and J. P. Arnaud. 1990. Batch fermentation with entrapped growing cells of Lactobacillus casei. Appl. Microbiol. Biotechnol. 32:403-408.
14. Lee, K. Y., and T. R. Heo. 1998. Identification of Bifidobacterium strains at the genus level by thin layer chromatographic determination of organic acids with culture broth of isolated bacteria strain from human feces. Food Biotechnol. 7:95-99.
15. Marteau, P., M. Minekus, R. Havenarr, and J. H. J. Huis Int Veld. 1997. Survival of lactic acid bacteria in a dynamic model of the stomach and small intestine: validation and the effects of bile. J. Dairy Sci. 80:1031-1037.
16. Pochart, P., P. Marteau, T. Bouhnik, I. Goderel, P. Bourlioux, and J. Rambaud. 1992. Survival of bifidobactetia ingested via fermented milk during their passage through the human small intestine: an in vivo study using intestinal perfusion. Am. J. Clin. Nutr. 55:78-80.
17. Prevost, H., and C. Divies. 1988. Continuous pre-fermentation of milk by entrapped yoghurt bacteria. I. Development of the process. Milchwissenschaft 43:621-625.
18. Rao, A. V., N. Shiwnarain, and J. Maharaj. 1989. Survival of microencapsulated Bifidobacterium pseudolongum in simulated gastric and intestinal juices. Can. Inst. Food. Sci. Technol. J. 22:345-349.
19. Scott, C. D. 1987. Immobilized cells: a review of recent literature. Enzyme Microb. Technol. 9:66-73.
20. Seifert, D., and A. Phillips. 1997. Production of small, monodispersed alginate beads for cell immobilization. Biotechnol. Prog. 13:562-568.
21. Sheu, T. Y., R. T. Marshall, and H. Heymann. 1993. Improving survival of culture bacteria in frozen desserts by microentrapment. J. Dairy Sci. 76: 1902-1907.
22. Sheu, T. Y., and R. T. Marshall. 1993. Microentrapment of lactobacilli in calcium alginate gels. J. Food Sci. 54:557-561.
23. Tanaka, H., M. Matsumura, and I. A. Veliky. 1984. Diffusion characteristics of substrates in Ca-alginate gel beads. Biotechnol. Bioeng. 26:53-58.