Two promoters regulate transcription of the mouse folylpolyglutamate synthetase geneThree tightly clustered Sp1 sites within the first intron markedly enhance activity of promoter B

Gene

Volumn 242, Issue 1-2, 2000, Pages 257-264

  Chen, J ^a   Hayes, P ^a   Roy, K ^a   Sirotnak, F M ^a,b  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b Weill Cornell Graduate School of Biomedical Sciences   (United States)

Author keywords

Folylpolyglutamate gene; Mouse; Promoter

Indexed keywords

FOLYLPOLYGLUTAMATE SYNTHASE; TRANSCRIPTION FACTOR SP1;

ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; DNA SEQUENCE; GENETIC ORGANIZATION; INTRON; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; SITE DIRECTED MUTAGENESIS; TRANSACTIVATION; TRANSCRIPTION REGULATION;

3T3 CELLS; ANIMALS; BASE SEQUENCE; BINDING SITES; CELL LINE; DEOXYRIBONUCLEASE I; DNA FOOTPRINTING; ENHANCER ELEMENTS (GENETICS); GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, REPORTER; INTRONS; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE SYNTHASES; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; SEQUENCE DELETION; SP1 TRANSCRIPTION FACTOR; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION, GENETIC; TUMOR CELLS, CULTURED;

ANIMALIA; MAMMALIA; MURINAE; RODENTIA;

EID: 0033979178     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00507-7     Document Type: Article

References (23)

1. Barredo J., Moran R.G. Determinants of antifolate cytotoxicity: folylpolyglutamate synthetase activity during cellular proliferations and development. Mol. Pharmacol. 42:1992;687-692.
2. Chen L., Qi H., Korenberg J., Garrow T.A., Choi Y.-J., Shane B. Purification and properties of human cytosolic folylpoly-gamma-glutamate synthetase and organization, localization, and differential splicing of its gene. J. Biol. Chem. 271:1996;13 077-13 087.
3. Courey A.J., Holtzman D.A., Jackson S.P., Tjian R. Synergistic activation by the glutamine-rich domains of human transcription factor Sp1. Cell. 59:1989;827-836.
4. Egan M.G., Sirlin S., Rumberger B.G., Garrow T.A., Shane B., Sirotnak F.M. Rapid decline in folylpolyglutamate synthetase activity and gene expression during maturation of HL-60 cells. Nature of the effect, impact on folate compound polyglutamate pools, and evidence for programmed down-regulation during maturation. J. Biol. Chem. 270:1995;5462-5468.
5. Fabre I., Fabre G., Goldman I.D. Polyglutamylation, an important element in methotrexate cytotoxicity and selective activity in tumor versus murine granulocytic progenitor cells in vitro. Cancer Res. 344:1984;3190-3195.
6. Freemantle S.J., Moran R.G. Transcription of the human folylpoly-gamma-glutamate synthetase gene. J. Biol. Chem. 272:1997;25 373-25 379.
7. Freemantle S.J., Taylor S.M., Krystal G., Moran R.G. Upstream organization of and multiple transcripts from the human folylpoly-gamma-glutamate synthetase gene. J. Biol. Chem. 270:1995;9579-9584.
8. Garrow T.A., Adman A., Shane B. Expression cloning of a human cDNA encoding folylpoly(gamma-glutamate) synthetase and determination of its primary structure. Proc. Natl. Acad. Sci. USA. 89:1992;9151-9155.
9. Kisliuk R.L. Pteroylpolyglutamates. Mol. Cell. Biochem. 39:1981;331-346.
10. Mastrangelo I.A., Courey A.Y., Wall J.S., Jackson S.P., Hough P.V. DNA looping and Sp1 multimer links: a mechanism for transcriptional synergism and enhancement. Proc. Natl. Acad. Sci. USA. 88:1991;5670-5674.
11. McBurney M.W., Whitmore G.F. Isolation and biochemical characterization of folate deficient mutants of Chinese hamster cells. Cell. 2:1974;173-182.
12. McGuire J.J., Coward J.K. Blakely R.L., Benkovic S.J. Folates and Pterins. vol. 1:1984;135-190 Wiley Interscience, New York.
13. McGuire J.J., Hsieh P., Coward J.K., Bertino J.R. Enzymatic synthesis of folylpolyglutamates. Characterization of the reaction and its products. J. Biol. Chem. 255:1980;5776-5788.
14. Moran R.G., Colman P.D. Mammalian folyl polyglutamate synthetase: partial purification and properties of the mouse liver enzyme. Biochemistry. 23:1984;4580-4589.
15. Pascal E., Tjian R. Different activation domains of Sp1 govern formation of multimers and mediate transcriptional synergism. Genes Dev. 5:1991;1646-1656.
16. Roy K., Mitsugi K., Sirotnak F.M. Organization and alternate splicing of the murine folylpolyglutamate synthetase gene. Different splice variants in L1210 cells encode mitochondrial or cytosolic forms of the enzyme. J. Biol. Chem. 271:1996;23 820-23 827.
17. Roy K., Mitsugi K., Sirotnak F.M. Additional organizational features of the murine folylpolyglutamate synthetase gene. Two remotely situated exons encoding an alternate 5′ end and proximal open reading frame under the control of a second promoter. J. Biol. Chem. 272:1997;5587-5593.
18. Samuels L.L., Moccio D.M., Sirotnak F.M. Similar differential for total polyglutamylation and cytotoxicity among various folate analogues in human and murine tumor cells in vitro. Cancer Res. 45:1985;1488-1495.
19. Shane B. Folylpolyglutamate synthesis and role in the regulation of one-carbon metabolism. Vitam. Horm. 45:1989;263-335.
20. Sirotnak F.M., DeGraw J.L. Selective antitumor action of folate analogs. Sirotnak F.M., Burchall J.J., Ensminger W.D., Montgomery J.A. Folate Antagonists as Therapeutic Agents. vol. 2:1984;43-91 Academic Press, New York.
21. Sirotnak F.M., Johnson T.B., Samuels L.L., Galivan J. Proliferation-dependency of folylpolyglutamyl synthetase activity in maturating luminal epithelial cells of mouse small intestine. Biochem. Pharmacol. 37:1988;4239-4241.
22. Su W., Jackson S., Tjian R., Echols H. DNA looping between sites for transcriptional activation: self-association of DNA-bound Sp1. Gene Dev. 5:1991;820-826.
23. Taylor S.M., Freemantle S.J., Moran R.G. Structural organization of the human folypoly-gamma-glutamate synthetase gene: evidence for a single genomic locus. Cancer Res. 55:1995;6030-6034.