In situ analysis of embryos with receptor or ligand fusion protein probes [1]

Current Biology

Volumn 10, Issue 2, 2000, Pages

  Flanagan, John G ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOPROBE; EMBRYO; HYBRID PROTEIN; LIGAND; RECEPTOR;

EID: 0033973463     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/s0960-9822(00)00293-1     Document Type: Letter

References (11)

1. Cheng H.-J., Flanagan J.G. Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases. Cell. 79:1994;157-168.
2. Cheng H.-J., Nakamoto M., Bergemann A.D., Flanagan J.G. Complementary gradients in expression and binding of ELF-1 and Mek4 in development of the topographic retinotectal projection map. Cell. 82:1995;371-381.
3. Flanagan J.G., Cheng H.J., Feldheim D., Hattori M., Lu Q., Vanderhaeghen P. Alkaline phosphatase fusions of ligands or receptors as in situ probes for staining of cells, tissues and embryos. Meth Enzymol. 2000;. in press.
4. Sobieszczuk D.F., Wilkinson D.G. Masking of Eph receptors and ephrins. Curr Biol. 9:1999;R469-R470.
5. Wilkinson D.G., Nieto M.A. Detection of messenger RNA by in situ hybridization to tissue sections and whole mounts. Meth Enzymol. 225:1993;361-373.
6. Harlow E., Lane D. Antibodies: A Laboratory Manual. 1988;Cold Spring Harbor Laboratory Press, Cold Spring Harbor Laboratory, New York.
7. Skarnes W.C. The identification of new genes: gene trapping in transgenic mice. Curr Opin Biotech. 4:1993;684-689.
8. Flanagan J.G., Leder P. The kit ligand: a cell surface molecule altered in Steel mutant fibroblasts. Cell. 63:1990;185-194.
9. Aruffo A., Stamenkovic I., Melnick M., Underhill C.B., Seed B. CD44 is the principal cell surface receptor for hyaluronate. Cell. 61:1990;1303-1313.
10. Gale N.W., Holland S.J., Valenzuela D.M., Flenniken A., Pan L., Ryan T.E., Henkemeyer M., Strebhardt K., Hirai H., Wilkinson D.G.et al. Eph receptors and ligands comprise two major specificity subclasses and are reciprocally compartmentalized during embryogenesis. Neuron. 17:1996;9-19.
11. Hornberger M.R., Dutting D., Ciossek T., Yamada T., Handwerker C., Lang S., Weth F., Huf J., Wessel R., Logan C.et al. Modulation of EphA receptor function by coexpressed ephrinA ligands on retinal ganglion cell axons. Neuron. 22:1999;731-742.