Cerebral metabolism of lactate in vivo: Evidence for neuronal pyruvate carboxylation

Journal of Cerebral Blood Flow and Metabolism

Volumn 20, Issue 2, 2000, Pages 327-336

  Hassel, Bjørnar ^a   Bråthe, Anders ^b  

^a NORWEGIAN DEFENCE RESEARCH ESTABLISHMENT FFI   (Norway)

^b UNIVERSITY OF OSLO   (Norway)

Author keywords

Anaplerosis; Cerebral metabolism; Glucose; Lactate; Malic enzyme; Pyruvate carboxylation

Indexed keywords

CARBON 13; CARBON 14; FUMARATE HYDRATASE; GLUCOSE; GLUTAMIC ACID; GLUTAMINE; LACTATE DEHYDROGENASE; LACTIC ACID; MALATE DEHYDROGENASE (DECARBOXYLATING); PYRUVIC ACID;

AMINO ACID ANALYSIS; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN METABOLISM; CARBON NUCLEAR MAGNETIC RESONANCE; CARBOXYLATION; ENZYME ACTIVITY; ENZYME ASSAY; FEMALE; MOUSE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL;

EID: 0033969656     PISSN: 0271678X     EISSN: None     Source Type: Journal    
DOI: 10.1097/00004647-200002000-00014     Document Type: Article

References (16)

1. Alston TA, Mela L, Bright HJ (1977) 3-Nitropropionate, the toxic substance of Indigofera, is a suicide inactivator of succinate dehydrogenase. Proc Natl Acad Sci USA 74:3767-3771
2. Bachelard HS, Badar Goffer RS (1993) NMR spectroscopy in neurochemistry. J Neurochem 61:412-429
3. 13C NMR. J Biol Chem 265:12916-12926
4. Cesar M, Hamprecht B (1995) Immunocytochemical examination of neural rat and mouse primary cultures using monoclonal antibodies raised against pyruvate carboxylase. J Neurochem 64:2312-2318
5. 13C nuclear magnetic resonance. Endocrinology 136:296-305
6. 2 fixation and metabolic compartmentation. Brain Res 38:355-370
7. Cotman CW, Hamberger AC (1978) Glutamate as a CNS neurotransmitter: properties of release, inactivation and biosynthesis. In: Amino acids as chemical transmitters (Fonnum F, ed), New York: Plenum Press, pp 379-412
8. Cremer JE, Cunningham VJ, Pardridge WM, Braun LD, WH (1979) Kinetics of blood-brain barrier transport of pyruvate, lactate and glucose in suckling, weanling and adult rats. J Neurochem 33:439-445
9. 1H-MRS investigation of brain metabolic changes during lactate-induced panic. Psychiatry Res 76:89-99
10. Edvinsson L, Nielsen KC, Owman C (1973) Orcadian rhythm in cerebral blood volume of mouse. Experientia 29:432-433
11. 13C-edited NMR spectroscopy. J Cereb Blood Flow Metab 10:170-179
12. Gibbs EL, Lennox WG, Nims LF, Gibbs FA (1943) Arterial and cerebral venous blood: arterial-venous differences in man. J Biol Chem 144:325-332
13. Gray EG, Wittaker VP (1962) The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived from homogenization and centrifugation. J Anat 96:79-88
14. Tsacapoulos M, Magistretti PJ (1996) Metabolic coupling between glia and neurons. J Neurosci 16:877-885
15. Van den Berg CJ, Krzalic LJ, Mela P, Waelsch H (1969) Compartmentation of glutamate metabolism in brain: evidence for the existence of two different tricarboxylic acid cycles in brain. Biochem J 113:281-290
16. Vogel R, Jennemann G, Seitz J, Wiesinger H, Hamprecht B (1998) Mitochondrial malic enzyme: purification from bovine brain, generation of an antiserum, and immunocytochemical localization in neurons of rat brain. J Neurochem 71:844-852