A novel, rapid, and accurate method for detecting microdeletion involving the DAZ gene in infertile men

Fertility and Sterility

Volumn 73, Issue 2, 2000, Pages 242-247

  Lucas, Hervé ^a   Patrat, Catherine ^a   Jouannet, Pierre ^a   Beldjord, Cherif ^b   Bienvenu, Thierry ^b  

^a Hôpital Cochin ^*  (France)

^b NONE

Author keywords

Denaturing gradient gel electrophoresis; Human DAZ gene; Infertility

Indexed keywords

ARTICLE; AZOOSPERMIA; CLINICAL ARTICLE; CYTOGENETICS; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA DETERMINATION; GENE DELETION; GENE ISOLATION; GENE LOCUS; HUMAN; HUMAN CELL; MALE; MALE INFERTILITY; NUCLEOTIDE SEQUENCE; OLIGOSPERMIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; Y CHROMOSOME;

ADULT; BASE SEQUENCE; CASE-CONTROL STUDIES; DNA MUTATIONAL ANALYSIS; ELECTROPHORESIS; FEMALE; HUMANS; INFERTILITY, MALE; MALE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROTEINS; RETROSPECTIVE STUDIES; RNA-BINDING PROTEINS; SEQUENCE DELETION; Y CHROMOSOME;

EID: 0033969071     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0015-0282(99)00504-X     Document Type: Article

References (14)

1. Tiepolo L., Zuffardi O. Localization of factors controlling spermatogenesis in the nonfluorescent portion of the human Y chromosome. Hum Genet. 34:1976;119-124.
2. Reijo R., Lee T.Y., Salo P., Alagappan R., Brown L.G., Rosenberg M., et al. Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA-binding protein gene. Nat Genet. 10:1995;383-392.
3. Reijo R., Alagappan R.K., Patrizio P., Page D.C. Severe oligozoospermia resulting from deletions of azoospermia factor gene on Y chromosome. Lancet. 347:1996;1290-1293.
4. Simoni M., Gromoll J., Dworniczak B., Rolf C., Abshagen K., Kamischke A., et al. Screening for deletions of the Y chromosome involving the DAZ (Deleted in AZoospermia) gene in azoospermia and severe oligozoospermia. Fertil Steril. 67:1997;542-547.
5. Saxena R., Brown L.G., Hawkins T., Alagappan R.K., Skaletsky H., Reeve M.P., et al. The DAZ gene cluster on the human Y chromosome arose from an autosomal gene that was transposed, repeatedly amplified and pruned. Nat Genet. 14:1996;292-299.
6. Patrizio P. Mapping of the Y chromosome and clinical consequence. Barratt C., De Jonge C., Mortimer D., Parinaud J. Genetics of human male infertility. 1997;25-42 Editions EDK, Paris.
7. WHO laboratory manual for the examination of human semen and semen-cervical mucus interaction. 3rd ed. 1992;3-27 Cambridge University Press, Cambridge.
8. David G., Bisson J.P., Czyglik F., Jouannet P., Gernigon C. Anomalies morphologiques du spermatozoïde humain et proposition pour un système de classification. J Gynecol Obstet Biol Reprod. 4:1975;17-36.
9. Seboun E., Barbaux S., Bourgeron T., Nishi S., Algonik A., Egashira M., et al. Gene sequence, localization, and evolutionary conservation of DAZLA, a candidate male sterility gene. Genomics. 41:1997;227-235.
10. Bienvenu T., Cazeneuve C., Kaplan J.C., Beldjord C. Mutation heterogeneity of cystic fibrosis in France screening by denaturing gradient gel electrophoresis using psoralen-modified oligonucleotide . Hum Mutat. 6:1995;23-29.
11. Yen P.H. A long-range restriction map of deletion interval 6 of the human Y chromosome a region frequently deleted in azoospermic males . Genomics. 54:1998;5-12.
12. Vogt P.H., Edelmann A., Kirsch S., Heneguriu O., Hirschmann P., Kiesewetter F., et al. Human Y chromosome azoospermia factor (AZF) mapped to different subregions in Yq11. Hum Mol Genet. 5:1996;933-943.
13. Stuppia L., Gatta V., Mastroprimiano G., Pompetti F., Calabrese G., Franchi F., et al. Clustering of Y chromosome deletions in subinternal E of internal 6 supports the existence of an oligozoospermia critical region outside the DAZ gene. J Med Genet. 34:1997;881-883.
14. Hoelfsloot L.H., Tuerlings J.H.A.M., Kremer J.A.M., Meuleman E.J.H. PCR analysis of Y-chromosome deletions in subinfertile men. [letter] Lancet. 349:1997;1400.