Anti-mucus polyclonal antibody production, purification and linkage to the surface of albumin microspheres

International Journal of Pharmaceutics

Volumn 195, Issue 1-2, 2000, Pages 147-158

  MacAdam, A B ^a   Shafi, Z B ^a   Marriott, C ^b   Martin, G P ^b   James, S L ^a  

^a UNIVERSITY OF BRIGHTON   (United Kingdom)

^b KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

35S anti rabbit IgG; Albumin microspheres; Antibody isolation; Antibody linkage; Antibody production; Bioadhesion; Bovine serum albumin; Carboxymethylated MS; Carboxymethylated mucus; ELISA; Ligand binding; Mucoadhesive strength; PAGE; Perfusion of rat jejunum segments; Polyclonal antibodies; Radio iodinated mucus

Indexed keywords

1 (3 DIMETHYLAMINOPROPYL) 3 ETHYLCARBODIIMIDE; ALBUMIN; BCG VACCINE; FREUND ADJUVANT; MICROSPHERE; MUCIN; POLYCLONAL ANTIBODY;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY PRODUCTION; ARTICLE; CONTROLLED STUDY; COVALENT BOND; CROSS REACTION; DRUG DELIVERY SYSTEM; GASTROINTESTINAL TRANSIT; HYDROPHILICITY; HYDROPHOBICITY; JEJUNUM; LIGAND BINDING; MALE; NONHUMAN; PRIORITY JOURNAL; RABBIT; RADIOIODINATION; RAT; STOMACH MUCUS;

ALBUMINS; ANIMALS; ANTIBODIES; CROSS REACTIONS; DRUG DELIVERY SYSTEMS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GASTROINTESTINAL TRANSIT; HUMANS; ILEUM; IMMUNOGLOBULIN G; MALE; MICE; MICROSPHERES; MUCINS; RABBITS; RATS; RATS, WISTAR; SURFACE PROPERTIES; SWINE; TIME FACTORS;

EID: 0033968688     PISSN: 03785173     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-5173(99)00390-7     Document Type: Article

References (22)

1. 2 and carbenoxolone. Gastroenterology. 80:1981;770-775.
2. Ezpeleta I., Irache J.M., Stainmesse S., Chabenat C., Gueguen J., Orecchioni A.M. Preparation of lectin-vicilin nanoparticle conjugates using the carbodiimide coupling technique. Int. J. Pharm. 142:1996;227-233.
3. Galisteogonzalez F., Martinrodriguez A., Hidalgoalvarez R. Adsorption of monoclonal IgG on polystyrene microspheres. Colloid Polym. Sci. 272(3):1994;352-358.
4. Grimsley P.G., Amos T.A., Gordon M.Y., Greaves M.F. Rapid positive selection of CD34+ cells using magnetic microspheres coated with monoclonal antibody QBEND/10 linked via a cleavable disulphide bond. Leukemia. 7(6):1993;898-908.
5. Ho S.B., Robertson A.M., Shekels L.L., Lyftogt C.T., Niehans G.A., Toribara N.W. Expression cloning of gastric mucin complementary DNA and localization of mucin gene expression. Gastroenterology. 109:1995;735-747.
6. Hughes, D.R.L., 1988. PhD thesis, Brighton Polytechnic.
7. Lehr C.M., Poelma F.G.J., Junginger H.E., Tukker J.J. An estimation of the turnover time of intestinal mucus. Int. J. Pharm. 70(3):1991;235-240.
8. Lehr C.M., Bouwstra J.A., Kok W., Deboer A.G., Tukker J.J., Verhoef J.C., Breimer D.D., Junginger H.E. Effects of the mucoadhesive polymer polycarbophil on the intestinal absorption of a peptide drug in the rat. J. Pharm. Pharmacol. 44(5):1992;402-407.
9. Lethem M.I., James S.L., Marriott C. The role of mucus glycoprotein in the rheologic properties of cystic fibrosis sputum. Am. Rev. Resp. Dis. 142:1990;1053-1058.
10. Longo W.E., Iwata H., Lindheimer T.A., Goldberg E.P. Preparation of hydrophilic microspheres using polymeric dispersing agents. J. Pharm. Sci. 71:1982;1323-1328.
11. MacAdam A.B., Shafi Z.B., Martin G.P., James S.L. Preparation of hydrophobic and hydrophilic MS and determination of surface carboxylic acid and amino residues. Int. J. Pharm. 151:1997;47-55.
12. Matsuo K., Ota H., Atamatsu T., Sugiyama A., Katsuyama T. Histochemistry of the surface mucous layer of the human colon. Gut. 40(6):1997;782-789.
13. Pimm M.V., Jones J.A., Price M.R., Middle J.G., Embleton M.J., Baldwin R.W. Tumour localisation of monoclonal antibodies against a rat mammary carcinoma and suppression of tumour growth with adriamycin-antibody conjugate. Cancer Immunol. Immunotherap. 12:1982;125-134.
14. Porchet N., Nguyen V.C., Dufosse J., Audie J.P., Guyonnet-Duperat V., Gross M.S., Dennis C., Degand P., Bernheim A., Aubert J.P. Molecular cloning and chromosomal localization of a novel human tracheobronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs. Biochem. Biophys. Res. Comm. 175:1991;414-422.
15. Shafi Z.B., Martin G.P., Olliff C.J., James S.L. Surface modification of albumin microspheres. J. Drug Target. 3:1995;53-56.
16. Shen R.S., Wang R.Z., Xing R.Y., Li Y.Q., Zhou F.Q., Jiang S.H., Lin Z.H., Xu B.L. Magnetic microparticle antibodies and their application to RIAs. J. Radioanal. Nuc. Chem. 206(2):1996;205-218.
17. Spicer A.P., Parry G., Patton S., Gendler S.J. Molecular-cloning and analysis of the mouse homolog of the tumor-associated mucin, MUC1, reveals conservation of potential o-glycosylation sites, transmembrane and cytoplasmic domains and a loss of minisatellite-like polymorphism. J. Biol. Chem. 266(23):1991;15099-15109.
18. Steinez M., Tamir S. An improved method to create nitrocellulose particles suitable for the immobilization of antigen and antibody. J. Immunol. Methods. 197(1):1995;171-177.
19. Smart J.D., Carpenter B., Mortazavi S.A. Is mucus dehydration an important factor in mucoadhesion. Proc. Int. Sympt. Release Bioact. Mater. 18:1991;629-630.
20. Tadikonda K.R., Davis R.H. Cell separations using targeted monoclonal antibodies against overproduced surface proteins. Appl. Biochem. Biotech. 45-6:1994;233-244.
21. Tirosh B., Rubinstein A. Migration of adhesive and nonadhesive particles in the rat intestine under altered mucus secretion conditions. J. Pharm. Sci. 87(4):1998;453-456.
22. Townes A.B., Martin G.P., James S.L., Marriott C. Microparticulate surface groups. J. Pharm. Pharmacol. (Suppl.). 41:1989;140P.