Crystallization and preliminary X-ray crystallographic studies of Thermus thermophilus HB8 MutM protein involved in repairs of oxidative DNA damage

Journal of Biochemistry

Volumn 127, Issue 1, 2000, Pages 9-11

  Sugahara, Mitsuaki ^a   Mikawa, Tsutomu ^a,d   Kato, Ryuichi ^a   Fukuyama, Keiichi ^a   Kumasaka, Takashi ^c   Yamamoto, Masaki ^c   Inoue, Yorinao ^c   Kuramitsu, Seiki ^a,b,c  

^a OSAKA UNIVERSITY   (Japan)

^b RIKEN YOKOHAMA INSTITUTE   (Japan)

^c RIKEN HARIMA INSTITUTE   (Japan)

^d INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

Author keywords

Crystallization; DNA repair enzyme; MutM protein; Oxidative DNA damage; Thermus thermophilus HB8; X ray diffraction

Indexed keywords

BACTERIAL PROTEIN;

ARTICLE; CRYSTALLIZATION; DNA DAMAGE; DNA DETERMINATION; NONHUMAN; OXIDATIVE STRESS; PROTEIN ANALYSIS; THERMUS THERMOPHILUS; X RAY CRYSTALLOGRAPHY;

BACTERIA (MICROORGANISMS); THERMUS THERMOPHILUS;

EID: 0033968401     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022588     Document Type: Article

References (14)

1. Friedberg, E.C., Walker, G.C., and Siede, W. (1995) DNA Repair and Mutagenesis, American Society of Microbiology Press, Washington, DC
2. van der Kemp, P.A., Thomas, D., Barbey, R., de Oliveira, R., and Boiteux, S. (1996) Cloning and expression in Escherichia coli of the OGG1 gene of Saccharomyces cerevisiae, which codes for a DNA glycosylase that excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine. Proc. Natl. Acad. Sci. USA 93, 5197-5202
3. Radicella, J.P., Dherin, C., Desmaze, C., Fox, M.S., and Boiteux, S. (1997) Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 94, 8010-8015
4. Aburatani, H., Hippo, Y., Ishida, T., Takashima, R., Matsuba, C., Kodama, T., Takao, M., Yasui, A., Yamamoto, K., and Asano, M. (1997) Cloning and characterization of mammalian 8-hydroxyguanine-specific DNA glycosylase/apurinic, apyrimidinic lyase, a functional mutM homologue. Cancer Res. 57, 2151-2156
5. Rosenquist, T.A., Zharkov, D.O., and Grollman, A.P. (1997) Cloning and characterization of a mammalian 8-oxoguanine DNA glycosylase. Proc. Natl. Acad. Sci. USA 94, 7429-7434
6. Arai, K., Morishita, K., Shinmura, K., Kohno, T., Kim, S.R., Nohmi, T., Taniwaki, M., Ohwada, S., and Yokota, J. (1997) Cloning of a human homolog of the yeast OGG1 gene that is involved in the repair of oxidative DNA damage. Oncogene 14, 2857-2861
7. Tchou, J., Kasai, H., Shibutani, S., Chung, M.H., Laval, J., Grollman, A.P., and Nishimura, S. (1991) 8-Oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity. Proc. Natl. Acad. Sci. USA 88, 4690-4694
8. Michaels, M.L., Pham, L., Cruz, C., and Miller, J.H., (1991) MutM, a protein that prevents G.C-T.A transversions, is formamidopyrimidine-DNA glycosylase. Nucleic Acids Res. 19, 3629-3632
9. 2-terminal proline acts as a nucleophile in the glycosylase/AP-lyase reaction catalyzed by Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg) protein. J. Biol. Chem. 272, 5335-5341
10. Rabow, L.E. and Kow, Y W. (1997) Mechanism of action of base release by Escherichia coli Fpg protein: role of lysine 155 in catalysis. Biochemistry 36, 5084-5096
11. Mikawa, T., Kato, R., Sugahara, M., and Kuramitsu, S. (1998) Thermostable repair enzyme for oxidative DNA damage from extremely thermophilic bacterium, Thermus thermophilus HB8. Nucleic Acids Res. 26, 903-910
12. Otwinowski, Z. and Minor, W. (1997) Processing of X ray-diffraction data collected in oscillation mode in Methods in Enzymology (Carter Jr., C.W. and Sweet, R.M., eds.) Vol. 276, pp. 307-326, Macromolecular Crystallography, part A, Academic Press, New York
13. Matthews, B.W. (1968) Solvent content of protein crystals. J. Mol. Biol. 33, 491-497
14. Yamamoto, M., Kumasaka, T., Fujisawa, T., and Ueki, T. (1998) Trichronic concept at SPring-8 RIKEN Beamline I. J. Synchrotron Rad. 5, 222-225