Virulence factors and genetic relatedness of Escherichia coli strains isolated from pigs with post-weaning diarrhea

Veterinary Microbiology

Volumn 71, Issue 3-4, 2000, Pages 211-222

  Osek, J ^a  

^a NATIONAL VETERINARY RESEARCH INSTITUTE   (Poland)

Author keywords

Diarrhea; ERIC PCR; Escherichia coli; Genetics; Pig Bacteria; RAPD PCR; Virulence markers

Indexed keywords

ARTICLE; BACTERIAL GENETICS; BACTERIAL VIRULENCE; BACTERIUM ISOLATION; DIARRHEA; DNA FINGERPRINTING; FIMBRIA; GENETIC POLYMORPHISM; GENOTYPE; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; STRAIN DIFFERENCE; SWINE;

AGGLUTINATION TESTS; ANIMALS; ANTIBODIES, BACTERIAL; BACTERIAL TOXINS; CONSENSUS SEQUENCE; DIARRHEA; DNA PRIMERS; DNA, BACTERIAL; ELECTROPHORESIS, AGAR GEL; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; FIMBRIAE, BACTERIAL; O ANTIGENS; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE; SEROTYPING; SHIGA TOXINS; SWINE; SWINE DISEASES; VIRULENCE;

EID: 0033967774     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1135(99)00168-6     Document Type: Article

References (41)

1. Aarestrup F.M., Jorsal S.E., Ahrens P., Jensen N.E., Meyling A. Molecular characterization of Escherichia coli strains isolated from pigs with edema disease. J. Clin. Microbiol. 35:1997;20-24.
2. Achtman M., Pluschke G. Clonal analysis of descent and virulence among selected Escherichia coli. Annu. Rev. Microbiol. 40:1986;185-210.
3. Bertschinger H.U., Bachmann M., Mettler C., Pospischil A., Schraner E.M., Stamm M., Sydler T., Wild P. Adhesive fimbriae produced in vivo by Escherichia coli O139:K12(B):H1 associated with enterotoxaemia in pigs. Vet. Microbiol. 25:1990;267-281.
4. Caugant D.A., Levin B.R., Orskov I., Orskov F., Eden C.S., Selander R.K. Genetic diversity in relation to serotype in Escherichia coli. Infect. Immun. 49:1985;407-413.
5. Cave H., Bingen E., Elion J., Denamur E. Differentiation of Escherichia coli strains using randomly amplified polymorphic DNA analysis. Res. Microbiol. 145:1994;141-150.
6. Chen J., Griffiths M.W. PCR differentiation of Escherichia coli from other Gram-negative bacteria using primers derived from the nucleotide sequences flanking the gene encoding the universal stress protein. Lett. Appl. Microbiol. 27:1998;369-371.
7. Della-Costa L.M., Irino K., Rodrigues J., Rivera I.N.G., Trabulsi L.R. Characterisation of diarroeagenic Escherichia coli clones by ribotyping and ERIC-PCR. J. Med. Microbiol. 47:1998;227-234.
8. Fekete A., Bantele J.A., Halling S.M., Stick W. Amplification fragment length polymorhism in Brucella strains by use of polymerase chain reaction with arbitrary primers. J. Bacteriol. 174:1992;7778-7783.
9. Foxal P.A., Hu L., Mobley H.T.L. Use of polymerase chain reaction amplified Helicobacter pylori urease structural genes for differentiation of isolates. J. Clin. Microbiol. 30:1992;739-741.
10. Gaastra W., de Graaf F.K. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiol. Rev. 46:1982;129-161.
11. Hulton C.S.J., Huggins C.F., Sharp P.M. ERIC sequences: a novel family of repetitive elements in the genomes of Escherichia coli, Salmonella typhimurium and other enterobacteria. Mol. Microbiol. 5:1991;825-834.
12. Hunter P.R., Gaston M.A. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J. Clin. Microbiol. 26:1988;2465-2466.
13. Imberechts H., De Greve H., Lintermans P. The pathogenesis of edema disease in swine. Vet. Microbiol. 31:1992;221-233.
14. Imberechts H., Wild P., Charlier G., De Greve H., Lintermans P., Pohl P. Characterization of F18 fimbrial genes fedE and fedF involved in adhesion and length of enterotoxemic Escherichia coli strain 107/86. Microbial. Pathogen. 21:1996;183-192.
15. Katsuda G.L., Sameshime T., Akiba M., Nakazawa M. Characterisation of Escherichia coli O157:H7 (H-) derived from cattle using random amplification of polymorphic DNA analysis. Res. Vet. Sci. 65:1998;177-178.
16. MacLeod D.L., Gyles C.L., Clarke R.C. Physicochemical and biological properties of purified Escherichia coli shiga-like toxin II variant. Infect. Immun. 59:1991;1300-1306.
17. Makino S., Okada Y., Maruyama T., Kaneko S., Sasakawa C. PCR-based random amplified polymorphic DNA fingerprinting of Yersinia pseudotuberculosis and its practical applications. J. Clin. Microbiol. 32:1994;65-69.
18. Maniatis, T., Fritsch, E.F., Sambrook, J. (Ed.), 1982. Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 68 pp.
19. Muzurier S.I., Wernas K. Typing of Listeria strains by random amplification of polymorphic DNA. Res. Microbiol. 143:1992;499-505.
20. Nagy B., Arp L.H., Moon H.W., Casey T.A. Colonization of the small intestine of weaned pigs by enterotoxigenic Escherichia coli that lack known colonization factors. Vet. Pathol. 29:1992;239-246.
21. Nagy B., Wilson R.A., Whittam T.S. Genetic diversity among Escherichia coli isolates carrying F18 genes from pigs with porcine post-weaning diarrhea and edema disease. J. Clin. Microbiol. 37:1999;1642-1645.
22. Ochman H., Selander R.K. Evidence for clonal population structure in Escherichia coli. Proc. Natl. Acad. Sci. USA. 81:1984;198-201.
23. Ojeniyi B., Ahrens P., Meyling A. Detection of fimbrial and toxin genes in Escherichia coli and their prevalence in piglets with diarrhea. The application of colony hybridization assay, polymerase chain reaction and phenotypic assays. J. Vet. Med. B. 41:1994;49-59.
24. Orskov I., Orskov F., Jann B., Jann K. Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol. Rev. 41:1977;667-710.
25. Osek J. Genetic diversity among Escherichia coli O149:K91 strains isolated from pigs with diarrhoea determined by randomly amplified polymorphic DNA analysis. Res. Vet. Sci. 67:1999;197-198.
26. Osek J. Prevalence of shiga toxin genes among Escherichia coli strains isolated from pigs. Vet. Rec. 145:1999;557-558.
27. Osek J., Gallien P., Truszczynski M., Protz D. The use of polymerase chain reaction for determination of virulence factors of Escherichia coli strains isolated from pigs in Poland. Comp. Immunol. Microbiol. Infect. Dis. 22:1999;163-174.
28. Osek J., Svennerholm A.M. Determination of K88 antigens and enterotoxins of Escherichia coli strains isolated from Polish piglets with diarrhea by the use of enzyme-linked immunosorbent assays. Vet. Microbiol. 29:1991;299-308.
29. Pacheco A.B.F., Guth B.E.C., De Almeida D.F., Ferreira L.C.S. Characterization of enterotoxigenic Escherichia coli by random amplification of polymorphic DNA. Res. Microbiol. 147:1996;175-182.
30. Pacheco A.B.F., Soares K.C., De Almeida D.F., Viboud G.I., Binstein N., Ferreira L.C.S. Clonal nature of enterotoxigenic Escherichia coli serotype O6:H16 revealed by randomly amplified polymorphic DNA analysis. J. Clin. Microbiol. 36:1998;2099-2102.
31. Rivera I.G., Chowdhury M.A.R., Huq A., Jacobs D., Martius M.T., Colwell R.R. Enterobacterial repetitive intergenic consensus sequences and the PCR to generate fingerprints of genomic DNAs from Vibrio cholerae O1, O139 and non-O1 strains. Appl. Environ. Microbiol. 61:1995;2898-2904.
32. Selander R.K., Levin B.R. Genetic diversity and structure in Escherichia coli populations. Science. 210:1980;545-547.
33. Sojka, W.J., 1965. Escherichia coli in domestic animals and poultry. Commonwealth Agricultural Bureaux, Farnham Royal, Buck, England, pp. 104-156.
34. Tompkins L.S. The use of molecular methods in infectious diseases. N. Engl. J. Med. 327:1992;1290-1297.
35. Truszczynski M., Ciosek D., Wittig W. Die Bedeutung der O- und K-Antigene sowie der Enterotoxine als Indikatoren der Pathogenitat der Escherichia-coli-Stamme vom Schwein (Leipzig). Arch. Exp. Vet-Med. 39:1985;470-491.
36. Tschape H., Bender L., Ott M., Wittig W., Hacker J. Restriction fragment length polymorphism and virulence pattern of the veterinary pathogen Escherichia coli O139:K82:H1. Zentralbl. Bakteriol. 276:1992;264-272.
37. Versalovic J., Koeuth T., Lupski J.R. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 19:1991;6823-6831.
38. Wang G., Whittam T.S., Berg C.M., Berg D.E. RAPD (arbitrary primer) PCR is more sensitive then multilocus enzyme electrophoresis for distinguishing related bacterial strains. Nucleic Acids Res. 21:1993;5930-5933.
39. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18:1990;7213-7218.
40. Williams J.G.K., Kubalik A.R., Livak K.J., Rafalski J.A., Tingey S.V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:1990;6531-6535.
41. Wittig W., Klie H., Gallien P., Lehmann S., Timm M., Tschape H. Prevalence of fimbrial antigens F18 and K88 and of enterotoxins and verotoxins among Escherichia coli isolated from weaned pigs. Zbl. Bakteriol. 283:1995;95-104.