Mechanisms of increased gluconeogenesis from alanine in rat isolated hepatocytes after endurance training

American Journal of Physiology - Endocrinology and Metabolism

Volumn 278, Issue 1 41-1, 2000, Pages

  Burelle, Y ^a   Fillipi, C ^b   Peronnet F ^a   Leverve, X ^b  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

^b UNIV GRENOBLE ALPES   (France)

Author keywords

2 oxoglutarate; Alanine aminotransferase; Alanine transport; Phosphoenolpyruvate pyruvate cycle

Indexed keywords

ALANINE; ALANINE AMINOTRANSFERASE; DIHYDROXYACETONE; GLUCOSE; LACTIC ACID; PHOSPHOENOLPYRUVATE; PYRUVIC ACID;

AMINO ACID TRANSPORT; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; GLUCONEOGENESIS; GLUCOSE BLOOD LEVEL; LIVER CELL; MALE; MITOCHONDRIAL RESPIRATION; NONHUMAN; PHYSICAL STRESS; PRIORITY JOURNAL; RAT; TRANSAMINATION;

EID: 0033966581     PISSN: 01931849     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpendo.2000.278.1.e35     Document Type: Article

References (42)

1. Ahlborg, G., P. Felig, L. Hagenfeldt, R. Hendler, and J. Wahren. Substrate turnover during prolonged exercise in man. Splanchnic and leg metabolism of glucose, free fatty acids, and amino acids. J. Clin. Invest. 53: 1080-1090, 1974.
2. Argaud, D., S. Halimi, F. Catelloni, and X. M. Leverve. Inhibition of gluconeogenesis in isolated hepatocytes after chronic treatment with phenobarbital. Biochem. J. 280: 663-669, 1991.
3. Argaud, D., H. Roth, N. Wiernsperger, and X. M. Leverve. Metformin decreases gluconeogenesis by enhancing the pyruvate kinase flux in isolated rat hepatocytes. Eur. J. Biochem. 213: 1341-1348, 1993.
4. Bergmeyer, H. U. Methods of Enzymatic Analysis (3rd ed.). New York: Academic, 1974.
5. Berry, M. N., and D. S. Friend. High yield preparation of isolated rat liver parenchymal cells. J. Cell. Biol. 43: 506-520, 1969.
6. Berry, M. N., E. Kun, and H. V. Werner. Regulatory role of reducing-equivalent transfer from substrate to oxygen in the hepatic metabolism of glycerol and sorbitol. Eur. J. Biochem. 33: 407-414, 1973.
7. Bobyleva-Guarriero, V., and H. A. Lardy. The role of malate in exercise-induced enhancement of mitochondrial respiration. Arch. Biochem. Biophys. 245: 470-476, 1986.
8. Boon, L., and A. J. Meijer. Role of amino-acid transport across the plasma membrane in the control of metabolic pathways in hepatocytes. In: Molecular Mechanisms of Transport, edited by E. Quagliariello and F. Palmieri. Amsterdam: Elsevier, 1992, p. 283-290.
9. Coggan, A. R., W. M. Kohrt, R. J. Spina, D. M. Bier, and J. O. Holloszy. Endurance training decreases plasma glucose turnover and oxidation during moderate-intensity exercise in men. J. Appl. Physiol. 68: 990-996, 1990.
10. Donovan, C. M., and K. D. Sumida. Training enhanced hepatic gluconeogenesis: the importance for glucose homeostasis during exercise. Med. Sci. Sports Exerc. 29: 628-634, 1997.
11. Donovan, C. M., and K. D. Sumida. Training improves glucose homeostasis in rats during exercise via glucose production. Am. J. Physiol. Regulatory Integrative Comp. Physiol. 258: R770-R776, 1990.
12. Drouin, R., C. Lavoie, J. Bourque, F. Ducros, D. Poisson, and J.-L. Chiasson. Increased hepatic glucose production response to glucagon in trained subjects. Am. J. Physiol. Endocrinol. Metab. 274: E23-E28, 1998.
13. Drouin, R., M. Milot, F. Péronnet, D. Massicotte, and C. Lavoie. Glucagon effect on hepatic glycogenolysis after training (Abstract). Can. J. Appl. Physiol. 23: 477, 1998.
14. Fafournoux, P., C. Rémésy, and C. Demigné. Control of alanine metabolism in rat liver by transport processes or cellular metabolism. Biochem. J. 210: 645-652, 1983.
15. Fell, D. Understanding the Control of Metabolism, edited by K. Snell. London: Portland, 1997, p. 301.
16. Felig, P., and J. Wahren. Amino acid metabolism in exercising man. J. Clin. Invest. 50: 2703-2714, 1971.
17. Groen, A. K., H. J. Sips, R. C. Vervoon, and J. M. Tager. Intracellular compartmentation and control of alanine metabolism in rat liver parenchymal cells. Eur. J. Biochem. 122: 87-93, 1982.
18. Groen, A. K., C. W. van Roermund, R. C. Vervoorn, and J. M. Tager. Control of gluconeogenesis in rat liver cells. Flux control coefficients of the enzymes in the gluconeogenic pathway in the absence and presence of glucagon. Biochem. J. 237: 379-389, 1986.
19. Horn, D. B., D. A. Podolin, J. E. Friedman, D. A. Scholnick, and R. S. Mazzeo. Alterations in key gluconeogenic regulators with age and endurance training. Metabolism 46: 414-419, 1997.
20. Huston, R. L., P. C. Weiser, G. L. Dohm, E. W. Askew, and J. B. Boyd. Effects of training, exercise and diet on muscle glycolysis and liver gluconeogenesis. Life Sci. 17: 369-376, 1975.
21. Ji, L. L., D. L. Lennon, R. G. Kochan, F. J. Nagle, and H. A. Lardy. Enzymatic adaptation to physical training under beta-blockade in the rat. Evidence of a beta 2-adrenergic mechanism in skeletal muscle. J. Clin. Invest. 78: 771-778, 1986.
22. Koivisto, V., R. Hendler, E. Nadel, and P. Felig. Influence of physical training on the fuel-hormone response to prolonged low intensity exercise. Metabolism 31: 192-197, 1982.
23. Kristensen, L. O., L. Sestoft, and M. Folke. Concentrative uptake of alanine in hepatocytes from fed and fasted rats. Am. J. Physiol. Gastrointest. Liver Physiol. 244: G491-G500, 1983.
24. Kunz, W. S., and E. J. Davis. Control of reversible intracellular transfer of reducing potential. Arch. Biochem. Biophys. 284: 40-46, 1991.
25. Leverve, X. M., A. K. Groen, A. J. Verhoeven, and J. M. Tager. Kinetic analysis of short-term effects of alpha-agonists on gluconeogenesis in isolated hepatocytes. FEBS Lett. 181: 43-46, 1985.
26. Malette, L. E., J. H. Exton, and C. R. Park. Effects of glucagon on amino acid transport and utilization in the perfused rat liver. J. Biol. Chem. 244: 5724-5728, 1969.
27. McGivan, J. D., J. C. Ramsell, and J. H. Lacey. Stimulation of alanine transport and metabolism by dibutyryl cyclic AMP in the hepatocytes from fed rats. Assessment of transport as a potential rate-limiting step for alanine metabolism. Biochim. Biophys. Acta 644: 295-304, 1981.
28. Mendenhall, L. A., S. C. Swanson, D. L. Habash, and A. R. Coggan. Ten days of exercise training reduces glucose production and utilization during moderate-intensity exercise. Am. J. Physiol. Endocrinol. Metab. 266: E136-E143, 1994.
29. Nicholls, D. G. The influence of respiration and ATP hydrolysis on the proton-electrochemical gradient across the inner membrane of rat-liver mitochondria as determined by ion distribution. Eur. J. Biochem. 50: 305-314, 1974.
30. Pilkis, S. J., and D. K. Granner. Molecular physiology of the regulation of hepatic gluconeogenesis and glycolysis. Annu. Rev. Physiol. 54: 885-909, 1992.
31. Podolin, D. A., T. T. Gleeson, and R. S. Mazzeo. Role of norepinephrine in hepatic gluconeogenesis: evidence of aging and training effects. Am. J. Physiol. Endocrinol. Metab. 267: E680-E686, 1994.
32. Rigoulet, M., X. M. Leverve, P. J. A. Plomp, and A. J. Meijer. Stimulation by glucose of gluconeogenesis in hepatocytes isolated from starved rats. Biochem. J. 245: 661-668, 1987.
33. Sumida, K. D., and C. M. Donovan. Enhanced hepatic gluconeogenic capacity for selected precursors after endurance training. J. Appl. Physiol. 79: 1883-1888, 1995.
34. Sumida, K. D., J. H. Urdiales, and C. M. Donovan. Enhanced gluconeogenesis from lactate in perfused livers after endurance training. J. Appl. Physiol. 74: 782-787, 1993.
35. Taguchi, S., Y. Hata, and K. Itoh. Enzymatic responses and adaptations to swimming training and hypobaric hypoxia in postnatal rats. Jpn. J. Physiol. 35: 1023-1032, 1985.
36. Turcotte, L. P., and G. A. Brooks. Effects of training on glucose metabolism of gluconeogenesis inhibited short-term-fasted rats. J. Appl. Physiol. 68: 944-954, 1990.
37. Van der Meer, R., and J. M. Tager. A simple method for the perifusion of isolated liver cells. FEBS Lett. 67: 36-40, 1976.
38. Wasserman, D. H., P. E. Williams, D. B. Lacy, D. R. Green, and A. D. Cherrington. Importance of intrahepatic mechanisms to gluconeogenesis from alanine during exercise and recovery. Am. J. Physiol. Endocrinol. Metab. 254: E518-E525, 1988.
39. Williamson, J. R., A. Jakob, and C. Refino. Control of the removal of reducing equivalents from the cytosol in perfused rat liver. J. Biol. Chem. 246: 7632-7641, 1971.
40. Williamson, J. R., P. Lund, and H. A. Krebs. The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver. Biochem. J. 103: 514-526, 1967.
41. Winder, W. W. Regulation of hepatic glucose production during exercise. Exerc. Sport Sci. Rev. 13:1-31, 1985.
42. Zuurendonk, P. F., and J. M. Tager. Rapid separation of particular components and soluble cytoplasm of isolated rat liver cells. Biochim. Biophys. Acta 333: 393-399, 1974.