Temporal analysis of connexin43 protein and gene expression throughout the menstrual cycle in human endometrium

Fertility and Sterility

Volumn 73, Issue 2, 2000, Pages 381-386

  Granot, Irit ^a   Dekel, Nava ^a   Bechor, Edna ^a   Segal, Ilana ^a   Fieldust, Sheila ^a   Barash, Amihai ^a  

^a KAPLAN HOSPITAL   (Israel)

Author keywords

Connexin43; Human endometrium; Menstrual cycle

Indexed keywords

CONNEXIN 43; ESTRADIOL; PROGESTERONE;

ADULT; ARTICLE; CLINICAL ARTICLE; ENDOMETRIUM; ENDOMETRIUM HYPERPLASIA; FEMALE; FOLLITROPIN RELEASE; GENE EXPRESSION; HUMAN; IMMUNOBLOTTING; LUTEINIZING HORMONE RELEASE; MENSTRUAL CYCLE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ADULT; CONNEXIN 43; ENDOMETRIUM; ESTRADIOL; FEMALE; FOLLICLE STIMULATING HORMONE; GENE EXPRESSION; GONADOTROPINS; HUMANS; INFERTILITY, FEMALE; LUTEINIZING HORMONE; MENSTRUAL CYCLE; PROGESTERONE; REFERENCE VALUES; STEROIDS;

EID: 0033963194     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0015-0282(99)00531-2     Document Type: Article

References (25)

1. Psycoyos A. Endocrine control of egg implantation. Greep R.O., Astwood E.B. Handbook of physiology. 1973;187-215 American Physiological Society, Washington, DC.
2. Cross J.C., Werb Z., Fisher S.J. Implantation and placenta key pieces of the development puzzle. Science. 266:1994;1508-1518.
3. Sharkey A. Cytokines and implantation. Rev Reprod. 3:1998;52-61.
4. Winterhager E., Grummer R., Jahn E., Willecke K., Traub O. Spatial and temporal expression of connexin26 and connexin43 in rat endometrium during trophoblast invasion. Dev Biol. 157:1993;399-409.
5. Gilula N.B., Reeves O.R., Steinbach A. Metabolic coupling, ionic coupling, and cell contacts. Nature. 253:1972;262-265.
6. Lowenstein W.R. Junctional intercellular communication the cell-to-cell membrane channel. Physiol Rev. 61:1981;829-913.
7. Grummer R., Chwalisz K., Mulholland J., Traub O., Winterhager E. Regulation of connexin26 and connexin43 expression in rat endometrium by ovarian steroid hormones. Biol Reprod. 51:1994;1109-1116.
8. Jahn E., Classen-Linke I., Kushe M., Beier H.M., Traub O., Grummer R., et al. Expression of gap junction connexin in the human endometrium throughout the menstrual cycle. Hum Reprod. 10:1995;2666-2670.
9. Regidor P.A., Regidor M., Schindler A.E., Winterhager E. Aberrant expression pattern of gap junction connexins in endometriotic tissues. Mol Hum Reprod. 3:1997;375-381.
10. Saito T., Oyamada M., Yamasaki H., Mori M., Kudo R. Co-ordinated expression of connexins 26 and 32 in human endometrial glandular epithelium during the reproductive cycle and the influence of hormone replacement therapy. Int J Cancer. 73:1997;479-485.
11. Risek B., Gutherie S., Kumar N., Gilula N.B. Modulation of gap junction transcript and protein expression during pregnancy in the rat. J Cell Biol. 110:1990;269-282.
12. Winterhager E., Stutenkemper R., Traub O., Beyer E., Willecke K. Expression of different connexin genes in rat uterus during decidualization and at term. Eur J Cell Biol. 55:1991;133-143.
13. Winterhager E., Brummer F., Dermietzel R., Husler D.F., Dender H.W. Gap junction formation in rabbit uterine epithelium in response to embryo recognition. Dev Biol. 126:1988;203-211.
14. Barash A., Weissman A., Manor M., Milman D., Ben-Arie A., Shoham Z. Prospective evaluation of endometrial thickness as a predictor of pituitary down-regulation following GnRH-a administration in an IVF program. Fertil Steril. 69:1998;496-499.
15. Weissman A., Barash A., Manor M., Ben-Arie A., Granot I., Shoham Z. Acute changes in endometrial thickness after aspiration of functional ovarian cysts. Fertil Steril. 69:1998;1142-1144.
16. Aplin J.D. The cell biology of human implantation. Placenta. 17:1996;269-275.
17. Granot I., Dekel N. Phosphorylation and expression of connexin-43 ovarian gap junction protein are regulated by luteinizing hormone. J Biol Chem. 268:1994;30502-30509.
18. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal of protein-dye binding. Anal Biochem. 72:1976;248-254.
19. Laemmli U.K. Cleavage of structural proteins during the assembly of the heat of T4 bacteriophage. Nature. 227:1970;680-685.
20. Chomczynski P., Sacchi N. Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem. 162:1987;156-159.
21. Orlando-Mathur C.E., Bechberger J.F., Goldberg G.S., Naus C.C.G., Kidder G.M., Kennedy T.G. Rat endometrial stromal cells express the gap junction genes connexin 26 and 43 and form functional gap junctions during in vitro decidualization. Biol Reprod. 54:1996;905-913.
22. Berg P.A., Navot D. The impact of embryonic development and endometrial maturity on the timing of implantation. Fertil Steril. 58:1992;537-542.
23. Nemansky M., Moy E., Lyons C.D., Yu I., Blithe D.L. Human endometrial stromal cells generate uncombined a-subunit from human chorionic gonadotropin, which can synergize with progesterone to induce decidualization. J Clin Endocrinol Metab. 83:1998;575-581.
24. Granot I., Dekel N. Development expression and regulation of the gap junction protein and transcript in rat ovaries. Mol Reprod Dev. 47:1997;231-239.
25. Reshef E., Lei Z.M., Rao V.C.H., Pridham D.D., Chegini N., Luborsky J.L. The presence of gonadotropin receptors in nonpregnant human uterus, human placenta, foetal membranes, and decidua. J Clin Endocrinol Metab. 70:1990;421-430.