Chain reaction cloning: A one-step method for directional ligation of multiple DNA fragments

Gene

Volumn 243, Issue 1-2, 2000, Pages 19-25

  Pachuk, Catherine J ^a   Samuel, Manoj ^a   Zurawski, John A ^b   Snyder, Linda ^c   Phillips, Paula ^a   Satishchandran, C ^a  

^a PFIZER INC   (United States)

^b UNIVERSITY OF PENNSYLVANIA SCHOOL OF VETERINARY MEDICINE   (United States)

^c CENTOCOR INC   (United States)

Author keywords

Bridge oligonucleotides; Multifragment ligation; Sequence specific ligation; Site specific ligation; Thermostable ligation

Indexed keywords

DNA FRAGMENT; OLIGONUCLEOTIDE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE;

ARTICLE; CLONING; GENOME; IN SITU HYBRIDIZATION; LIGATION; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION MAPPING; TEMPERATURE;

CLONING, MOLECULAR; DNA; DNA LIGASES; DNA POLYMERASE I; OLIGONUCLEOTIDES; PLASMIDS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS;

EID: 0033961872     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00508-9     Document Type: Article

References (4)

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4. Sutton D.W., Havstad P.K., Kemp J.D. Transgenic Res. 1:1992;228-236.