Analysis of chromosome loss and chromosome segregation in cytokinesis-blocked human lymphocytes: Non-disjunction is the prevalent mistake in chromosome segregation produced by low dose exposure to ionizing radiation

Mutagenesis

Volumn 15, Issue 1, 2000, Pages 1-7

  Touil, Nadia ^a   Elhajouji, Azeddine ^b   Thierens, Hubert ^c   Kirsch Volders, Micheline ^a  

^a FREE UNIVERSITY OF BRUSSELS   (Belgium)

^b NOVARTIS PHARMA AG   (Switzerland)

^c GHENT UNIVERSITY   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ANEUPLOIDY; ARTICLE; CHROMATID; CHROMOSOME 1; CHROMOSOME 17; CHROMOSOME LOSS; CHROMOSOME SEGREGATION; CYTOKINESIS; FEMALE; GAMMA RADIATION; HUMAN; HUMAN CELL; IONIZING RADIATION; LYMPHOCYTE; MALE; MICRONUCLEUS; NONDISJUNCTION; NORMAL HUMAN; PRIORITY JOURNAL; RADIATION DOSE; STATISTICS; X RAY;

EID: 0033960365     PISSN: 02678357     EISSN: None     Source Type: Journal    
DOI: 10.1093/mutage/15.1.1     Document Type: Article

References (44)

1. Alberman, E., Polani, P.E., Roberts, J.A.F., Spicer, C.C., Elliot, M. and Armstrong, E. (1972) Parental exposure to X-irradiation and Down's syndrome. Ann. Hum. Genet., 36, 95-208.
2. Boei, J.J.W.A. and Natarajan, A.T. (1995) Detection of chromosome malsegregation to the daughter nuclei in cytokinesis-blocked transgenic mouse splenocytes. Chromosome Res., 3, 45-53.
3. Bond, D.J. and Chandley, A.C. (1983) Aneuploidy. Oxford University Press, New York, NY.
4. Cornforth, M.N. and Goodwin, E.H. (1991) Transmission of radiation-induced acentric chromosomal fragments to micronuclei in normal fibroblasts. Radiat. Res., 126, 210-217.
5. Danford, N. (1984) Measurement of levels of aneuploidy in mammalian cells using a modified hypotonie treatement. Mutat. Res., 139, 127-132.
6. Darroudi, F., Farouqi, Z., Benova, D. and Natarajan, A.T. (1992) The mouse splenocyte assay, an in vivolin vitro system for biological monitoring: studies with X-rays, fission neutrons and bleomycin. Mutat. Res., 272, 237-248.
7. Darroudi, F., Meijers, C.M., Hadjidekova, V. and Natarajan, A.T. (1996) Detection of aneugenic and clastogenic potential of X-rays, directly and indirectly acting chemicals in human hepatoma (HepG2) and peripheral blood lymphocytes using the micronucleus assay and fluorescent in situ hybridization with a DNA centromeric probe. Mutagenesis, 11, 425-433.
8. Eastmond, D.A. and Tucker, J.D. (1989) Identification of aneuploidy inducing agents using cytokinesis-blocked human lymphocytes and an antikinetochore antibody. Environ. Mol. Mutagen., 13, 34-43.
9. Elhajouji, A., Van Hummelen, P. and Kirsch-Volders, M. (1995) Induction for a threshold of chemically induced aneuploidy in vitro in human lymphocytes. Environ. Mol. Mutagen., 26, 292-304.
10. Elhajouji, A., Tibaldi, F. and Kirsch-Volders, M. (1997) Indication for thresholds of chromosome non-disjunction versus chromosome lagging induced by spindle inhibitors in vitro in human lymphocytes. Mutagenesis, 12, 133-140.
11. Farouqi, Z., Darroudi, F. and Natarajan, A.T. (1993) The use of fluorescence in situ hybridization for the detection of aneugens in cytokinesis-blocked mouse splenocytes. Mutagenesis, 8, 329-334.
12. Fenech, M. and Morley, A.A. (1986) Cytokinesis-block micronucleus method in human lymphocytes: effect of in vivo ageing and low dose X-irradiation. Mutat. Res., 161, 193-198.
13. Goswani, P.C., Hill, M., Higashikubo, R., Wright, W.D. and Roti Roti, J.L. (1992) The suppression of the synthesis of a nuclear protein in cells blocked in G2 phase: identification of NP-170 as topoisomerase II. Radiat. Res., 132, 162-167.
14. Heddle, J.A., Hite, M., Kikhart, B., Mavournin, K., MacGregor, J.T., Nawell, G.W. and Salamone, M.F. (1983) The induction of micronuclei as a measure of genotoxicity: a report of the US Environmental Protection Agency Gene-Tox Program. Mutat. Res., 123, 61-118.
15. Hetch, F. and Hetch, B.K. (1987) Aneuploidy in humans: dimensions, demography and danger of abnormal numbers of chromosomes. In Vig, B.K. and Sandbers, A.A. (eds), Aneuploidy. Part A: Incidence and Etiology. Liss, New York, NY, pp. 9-50.
16. Hsieht, T. (1990) DNA topoisomerases. Curr. Opin. Cell Biol., 2, 461-463.
17. Johnson, G.D. and Nogueira Araujo, G.M.deC. (1981) A simple method of reducing the fading of immunoflouorescence during microscopy. J. Immunol. Methods, 43, 349-350.
18. Kirsch-Volders, M. (1986) Differential staining of chromosomes and spindle cannot be used as an assay to determine the effects of cancer promoters on primary cultures of human fibrobroblasts. Mutat. Res., 171, 177-183.
19. Kirsch-Volders, M. (1997) Towards a validation of the micronucleus test. Mutat. Res., 392, 1-4.
20. Kirsch-Volders, M., Tallon, I., Tanzarella, C., Sgura, A., Hermine, T., Parry, E.M. and Parry, J.M. (1996) Mitotic non-disjunction as a mechanism for in vitro aneuploidy induction by X-rays in primary human cells. Mutagenesis., 4, 307-313.
21. Lloyd, D.C., Edwards, A.A., Prosser, J.S., Finnon, P. and Moquet, J.E. (1988) In vitro induction of chromosomal aberrations in human lymphocytes with and without boron 10 by radiations concerned in boron neutron capture therapy. Br. J. Radiol., 61, 1136-1141.
22. Meyne, J., Littlefield, L.G. and Moyzis, R.K. (1989) Labelling of human centromeres using an alphoid DNA consensus sequence: application to the scoring of chromosome aberrations. Mutat. Res., 226, 75-79.
23. Migliori, L., Parrini, M., Sbrane, I., Biagni, C., Battaglia, A. and Loprieno, N. (1991) Micronucleated lymphocytes in people occupationally exposed to potential environmental contaminants: the age effect. Mutat. Res., 256, 13-19.
24. Miller, B.M., Werner, T., Weier, H.U. and Nusse, M. (1992) Analysis of radiation induced micronuclei by fluorescence in situ hybridization (FISH) simultaneously using telomeric and centromeric DNA probes. Radiat. Res., 131, 177-185.
25. Mormiter, P.K., Contopoulou, R. and Schild, D. (1981) Mitotic chromosome loss in a radiation-sensitive strain of the yeast Saccharomvces cerevisiae. Proc. Natl Acad. Sci. USA, 78, 5778-5782.
26. Natarajan, A.T., Vyas, R.C., Wiegant, J. and Curado, M.P. (1991) A cytogenetic follow-up study of the victims of a radiation accident in Goiania (Brazil). Mutat. Res., 247, 103-111.
27. Natarajan, A.T., Farooqi, Z., Darroudi, F., Banajee, A.S., Boei, J.J.W.A., Mullenders, L.H.F. and Gossen, J.A. (1993) In vivo assays for the detection and evaluation of aneugens. In Vig, B.K. (ed.), Chromosome Segregation and Aneuploidy. Springer, Berlin, Germany, pp. 363-376.
28. Natarajan, A.T., Balajee, A.S., Boei, J.J., Chatterjee, S, Darroudi, F., Grigorova, M., Noditi, M., Oh, H.J., Slijepcevic, P. and Vermeulen, S. (1994) Recent developments in the assessment of chromosomal damage. Int. J. Radiat. Biol., 66, 615-623.
29. Natarajan, A.T., Boei, J.J.W.A., Darroudi, F., Van Diemen, P.C.M., Dulout, F., Hande, M.P. and Ramalho, A.T. (1996) Current cytogenetic methods for detecting exposure and effects of mutagens and carcinogens. Environ. Health Perspect., 104 (suppl. 3), 445-448.
30. Noppre, H., Renzi, L. and Lindholm, C. (1993) Detection of whole chromosomes in micronuclei of cytokinesis-blocked human lymphocytes by antikinetochore staining and in situ hybridization. Mutagenesis, 8, 519-525.
31. Oshimura, M. and Barret, J.C. (1986) Chemically induced aneuploidy in mammalian cells: mechanisms and biological significance in cancer. Environ. Mutagen., 83, 129-159.
32. Parry, E.M., Danford, N. and Parry, J.M. (1982) Differential staining of chromosomes and spindle and its use as an assay for determining the effect of diethylstilboestrol on cultured mammalian cells. Mutat. Res., 105, 243-252.
33. Ramirez, M.J., Surrlles, J., Galofre, P., Creus, A. and Marcos, R. (1997) Radioactive iodine induces clastogenic and age-dependent aneugenic effects in lymphocytes of thyroid cancer patients as revealed by interhase FISH. Mutagenesis, 12, 449-455.
34. Schuessler, H. and Herget, A. (1980) Oxygen effect in the radiolysis of proteins. I. LDH. Int. J. Radiat. Biol., 37, 71-80.
35. Schuessler, H. and Schilling, K. (1984) Oxygen effect in the radiolysis of proteins. II. BSA. Int. J. Radiat. Biol., 45, 267-281.
36. Tallon, I., Verschaeve, L. and Kirsch-Volders, M. (1998) Cell cycle dependent aneuploidy induction by X-rays in vitro in human lymphocytes. Microsc. Res. Technique, 40, 344-353.
37. Thierens, H., Vral, A., De Ridder, L., Touil, N., Kirsch-Volders, M., Lambert, V. and Laurent, C. (1999) Scoring of different cytogenetic endpoints after in vitro low dose gamma-exposure: interlaboratory comparison for biomonitoring of radiological workers. Int. J. Radiat. Biol., 75, 23-24.
38. Uchida, I.A., Lee, C.P.V. and Byrnes, E.M. (1975) Chromosome aberrations induced in vitro by low doses of radiation: non-disjunction in lymphocytes of young adults. Am. J. Hum. Genet., 27, 419-429.
39. Vanderkerken, K., Vanparys, Ph., Verschaeve, L. and Kirsch-Volders, M. (1989) The mouse bone marrow micronucleus assay can be used to distinguish aneugens from clastogens. Mutagenesis, 4, 16-11.
40. Van Hummelen, P. and Kirsch-Volders, M. (1990) An improved method for the in vitro micronucleus test on human lymphocytes. Mutagenesis, 5, 203-204.
41. Van Hummelen, P., Deleener, A., Van Parys, P.H. and Kirsch-Volders, M. (1992) Discrimination of aneuploidogens from clastogens by C-banding DNA and area measurements of micronuclei from mouse bone marrow. Mutat. Res., 271, 13-28.
42. Vig, B.K. (1984) Sequence of centromere separation: another mechanism for the origin of non-disjunction. Hum. Genet., 66, 239-243.
43. Warters, R.L., Lyons, B.W., Kinnedy, K. and Mua, L.T. (1989) Topoisomerase in irradiated mammalian cells. Mutat. Res., 216, 43-55.
44. Zijno, A., Marcon, F., Leopardi, P. and Crebelli, R. (1994) Simultaneous detection of X-chromosome loss and non-disjunction in cytokinesis-blocked human lymphocytes with a centromeric DNA probe; implications for the human lymphocytes in vitro micronucleus assay using cytocahlasin B. Mutagenesis, 9, 225-232.