Affinity cleavage at the divalent metal site of porcine NAD-specific isocitrate dehydrogenase

Protein Science

Volumn 9, Issue 1, 2000, Pages 104-111

  Huang, Yu Chu ^a   Soundar, Sambanthamurthy ^a   Colman, Roberta F ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

Affinity cleavage; Isocitrate dehydrogenase; Metal site

Indexed keywords

ISOCITRATE DEHYDROGENASE; MANGANESE; NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; BINDING AFFINITY; BINDING SITE; ENZYME SPECIFICITY; MITOCHONDRIAL RESPIRATION; NONHUMAN; PRIORITY JOURNAL; SWINE;

SUIDAE; SUS SCROFA;

EID: 0033960110     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.9.1.104     Document Type: Article

References (32)

1. Amid A, Levine RL, Tsai L, Stadtman ER. 1989. Conversion of amino acid residues in proteins and amino acid homopolymers to carbonyl derivatives by metal-catalyzed oxidation reactions. J Biol Chem 264:3341-3346.
2. 2+-mediated oxidative cleavage. J Biol Chem 273:33002-33010.
3. Chen H, Huang Y-C, Colman RF. 1998. Identification of the subunit and important target peptides of pig heart NAD-dependent isocitrate dehydrogenase modified by the affinity label adenosine 5′-0-[S-(4-bromo-2,3-dioxobutyl)]thiophosphate. Biochemistry 37:6541-6551.
4. Cohen PF, Colman RF. 1972. Diphosphopyridine nucleotide dependent isocitrate dehydrogenase from pig heart. Characterization of the active substrate and modes of regulation. Biochemistry 11:1501-1508.
5. Cohen PF, Colman RF. 1974. Role of manganous ion in the kinetics of pig heart NAD-specific isocitrate dehydrogenase. Eur J Biochem 47:35-45.
6. Ehrlich RS, Colman RF. 1981. Binding of ligands to half of subunits of NAD-dependent isocitrate dehydrogenase from pig heart. Binding of manganous ion, isocitrate ADP and NAD. J Biol Chem 256:1276-1282.
7. Ehrlich RS, Colman RF. 1982. Interrelationships among nucleotide binding sites of pig heart NAD-dependent isocitrate dehydrogenase. J Biol Chem 257: 4769-4774.
8. Ehrlich RS, Colman RF. 1983. Separation, recombination and characterization of dissimilar subunits of the DPN-dependent isocitrate dehydrogenase from pig heart. J Biol Chem 258:7079-7086.
9. Ehrlich RS, Hayman S, Ramachandran N, Colman RF. 1981. Re-evaluation of molecular weight of pig heart NAD-specific isocitrate dehydrogenase. J Biol Chem 256:10560-10564.
10. Gallagher J, Zelenko O, Walts AD, Sigman DS. 1998. Protease activity of 1,10-phenothroline copper(I) targeted scission of the catalytic site of carbonic anhydrase. Biochemistry 37:2096-2104.
11. Hlavaty JJ, Nowak T. 1997. Affinity cleavage at the metal-binding site of phosphoenolpyruvate carboxykinase. Biochemistry 36:15514-15525.
12. +-dependent isocitrate dehydrogenase. J Biol Chem 264:12208-12214.
13. Huang Y-C, Colman RF. 1990. Subunit location and sequences of the cysteinyl peptides of pig heart NAD-dependent isocitrate dehydrogenase. Biochemistry 29:8266-8273.
14. Huang Y-C, Kumar A, Colman RF. 1997. Identification of the subunits and target peptides of pig heart NAD-specific isocitrate dehydrogenase modified by the affinity label 8-(4-bromo-2,3-dioxobutylthio)NAD. Arch Biochem Biophys 348:207-218.
15. + complexes. Biochemistry 30: 8671-8678.
16. Inglis AS. 1983. Cleavage at aspartic acid. Methods Enzymol 91:324-332.
17. Kim K, Rhee SG, Stadtman ER. 1985. Non-enzymatic cleavage of proteins by reactive oxygen species generated by dithiothreitol and iron. J Biol Chem 260:15394-15397.
18. Kim Y-O, Oh I-U, Park H-S, Jeng J, Song BJ, Huh T-L. 1995. Characterization of a cDNA clone for human NAD-specific isocitrate dehydrogenase α-subunit and structural comparison with its isoenzymes from different species. Biochem J 308:63-68.
19. Levine RL. 1983. Oxidative modification of glutamine synthetase. I. Inactivation is due to loss of one histidine. J Biol Chem 258:11823-11827.
20. Lundblad RL, Noyes CM. 1984. Chemical reagents for protein modification. Volume I. Boca Raton, Florida: CRC Press. p 72.
21. Miyake R, Murakami K, Owens JT, Greiner DP, Ozoline ON, Ishihama A, Meares CF. 1998. Dimeric association of E. coli RNA polymerase α subunits studied by cleavage of single-cysteine α subunits conjugated to iron-(s)-1-[p-(bromoacetamido)benzyl]ethylenediamine-tetraacetate. Biochemistry 37:1344-1349.
22. Nichols BJ, Hull L, Perry ACF, Denton RM. 1993. Molecular cloning and deduced amino acid sequences of the gamma subunits of rat and monkey NAD(+)-isocitrate dehydrogenases. Biochem J 295:347-350.
23. Nichols BJ, Perry ACF, Hall L, Denton RM. 1995. Molecular cloning and deduced amino acid sequences of the alpha and beta subunits of mammalian NAD(+)-isocitrate dehydrogenase. Biochem J 310:917-922.
24. Ramachandran N, Colman RF. 1978. Evidence for the presence of two non-identical subunits in NAD-dependent isocitrate dehydrogenase of pig heart. Proc Nat Acad Sci 75:252-255.
25. Ramachandran N, Colman RF. 1980. Chemical characterization of distinct subunits of pig heart DPN-specific isocitrate dehydrogenase. J Biol Chem 255:8859-8864.
26. Rana TM, Meares CF. 1991. Iron chelate mediated proteolysis: Protein-structure dependence. J Am Chem Soc 113:1859-1861.
27. Schepartz A, Cuenoud B. 1990. Site-specific cleavage of the protein calmodulin using a trifluoperazine-based affinity reagent. J Am Chem Soc 112:3247-3249.
28. Sigman DS, Chen CB. 1990. Chemical nucleases. Annu Rev Biochem 59:207-236.
29. 2+-isocitrate. J Biol Chem 268:5264-5271.
30. Wei CH, Chou W-Y, Chang G-G. 1995. Identification of Asp258 as the metal coordinate of pigeon liver malic enzyme by site-specific mutagenesis. Biochemistry 34:7949-7954.
31. 2+-ascorbate system. Biochemistry 33:7931-7936.
32. Zeng Y, Weiss C, Yao TG, Huang JM, Siconolfi-Baez L, Hsu P, Rushbrook JI. 1995. Isocitrate dehydrogenase from bovine heart: Primary structure of subunit 3/4. Biochem J 310:507-516.