New approach for separating Bacillus subtilis metalloprotease and α-amylase by affinity chromatography and for purifying neutral protease by hydrophobic chromatography

Journal of Chromatography B: Biomedical Sciences and Applications

Volumn 737, Issue 1-2, 2000, Pages 277-284

  Lauer, Isabelle ^a   Bonnewitz, Béatrice ^a   Meunier, Agnès ^b   Beverini, Marc ^a  

^a Ctr des Rech Nucleaires   (France)

^b Centre Jean Theves   (France)

Author keywords

Amylase; Bacillus subtilis; Enzymes; Metalloprotease; Protease; Purification

Indexed keywords

AMYLASE; METALLOPROTEINASE; NEUTRAL PROTEINASE;

AFFINITY CHROMATOGRAPHY; BACILLUS SUBTILIS; CHROMATOGRAPHY; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; TECHNIQUE; ULTRAVIOLET SPECTROPHOTOMETRY;

ALPHA-AMYLASE; BACILLUS SUBTILIS; CALCIUM; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; METALLOENDOPEPTIDASES; TEMPERATURE;

EID: 0033958282     PISSN: 13872273     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-4347(99)00482-X     Document Type: Conference Paper

References (26)

1. de la Guérivière J.F. Ann. Technol. Agric. 21:1972;253.
2. Ishida K., Nagasaki M. J. Jap. Soc. Food Sci. Technol. 36:1989;964.
3. Sai Manohar R., Haridas Rao P. Adv. Food Sci. 19:1997;15.
4. Tsuru D., McConn J.D., Yasunobu K.T. Bioch. Biophys. Res. Commun. 15:1964;367.
5. Tsuru D., Kira H., Yamamoto T., Fukumoto J. Agr. Biol. Chem. 30:1966;856.
6. Yasunobu K.T., McConn J.C. Methods in Enzymol. 19:1970;569.
7. Kobayashi R., Yoshimoto T., Tsuru D. Agric. Biol. Chem. 53:1989;2737.
8. McConn J.D., Tsuru D., Yasunobu K.T. J. Biol. Chem. 239:1964;3706.
9. Tsuru D., Kira H., Yamamoto T. Agr. Biol. Chem. 31:1967;718.
10. Fisher E.H., Stein E.A. Boyer P.D., Lardy H., Myrback M. The Enzymes. 2nd Edition :1960;313 Academic Press.
11. Fisher E.H., Sumerwell W.N., Junge J.M., Stein E.A. Proceedings of the VIIIth Symposium of the IVth Internat. Congress of Biochemistry, Vienna :1958;Pergamon Press, London.
12. Vallee B.L., Stein E.A., Summerwell W.N., Fisher E.H. J. Biol. Chem. 234:1959;2901.
13. Delecourt R. Ann. Technol. Agric. 23:1974;193.
14. Keay L. Biochem. Biophys. Res. Commun. 36:1969;257.
15. Schmidt A.S., Ventom A.M., Asenjo J.A. Enzyme Microb. Technol. 16:1994;131.
16. Safwat M.S.A., Mahmoud S.A.Z., Abdel Nasser M., Attia R.M., Ali F.S. Zbl. Mikrobiol. 138:1983;247.
17. Stredansky M., Kremnicky L., Sturdik E. Biotechnol. Techn. 7:1993;69.
18. Chang Q.L., Chen J.Y. Chem. Engrg. J. 59:1995;303.
19. Bradford M. Anal. Biochem. 72:1976;248.
20. Laemmli U.K. Nature. 227:1970;680.
21. Stein E.A., Fisher E.H. Biochim. Biophys. Acta. 39:1960;287.
22. Melander W., Horwath C. Arch. Biochem. Biophys. 183:1977;200.
23. Signor G., Vita C., Fontana A., Frigerio F., Bolognesi M. Eur. J. Biochem. 189:1990;221.
24. Yamane K., Maruo B. J. Bacteriol. 120:1974;792.
25. Detera S.D., Friedberg F. Inst. J. Peptide Protein Res. 14:1979;364.
26. Tsuru D., Kira H., Yamamo T., Fukumoto J. Agr. Biol. Chem. 30:1966;651.