Inhibitory effect of antisense epidermal growth factor receptor RNA on the proliferation of rat C6 glioma cells in vitro and in vivo

Journal of Neurosurgery

Volumn 92, Issue 1, 2000, Pages 132-139

  Pu, Peiyu ^a   Liu, Xuwen ^a,c   Liu, Aixue ^a,b   Cui, Jianling ^a   Zhang, Yunting ^a  

^a TIANJIN MEDICAL UNIVERSITY   (China)

^b WISTAR INSTITUTE   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Antisense epidermal growth factor receptor RNA; Gene therapy; Rat; Rat C6 glioma

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL PROLIFERATION; GENE TARGETING; GENE THERAPY; GLIOMA; GLIOMA CELL; HISTOPATHOLOGY; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; RAT; SOUTHERN BLOTTING; SURVIVAL TIME;

EID: 0033957751     PISSN: 00223085     EISSN: None     Source Type: Journal    
DOI: 10.3171/jns.2000.92.1.0132     Document Type: Article

References (14)

1. Agosti RM, Leuthold M, Gullick WJ, et al: Expression of the epidermal growth factor receptor in astrocytic tumours is specifically associated with glioblastoma multiforme. Virchows Arch (A) Pathol Anat 420:321-325, 1992
2. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinum thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159, 1987
3. Downward J, Yarden Y, Mayes E, et al: Close similarity of epidermal growth factor receptor and V-erb-B oncogene protein sequences. Nature 307:521-527, 1984
4. Ekstrand AJ, James CD, Cavenee WK, et al: Genes for epidermal growth factor receptor, transforming growth factor a and epidermal growth factor and their expression in human gliomas in vivo. Cancer Res 51:2164-2172, 1991
5. Kajiwara K, Nishizaki T, Orita T, et al: Silver colloid staining technique for analysis of glioma malignancy. J Neurosurg 73: 113-117, 1990
6. Libermann TA, Nusbaum HR, Razon N, et al: Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin. Nature 313:144-147, 1985
7. Liu X, Pu P: Expression of epidermal growth factor receptor in human gliomas. J Neurochem 67 (Suppl):S11A, 1996 (Abstract)
8. Liu XW, Pu PY, Gao ZX, et al: [Study on the expression of epidermal growth factor receptor gene in human gliomas.] Chin J Neurosurg 14:71-74, 1998 (Chin)
9. Maiden LT, Novak U, Kaye AH, et al: Selective amplification of the cytoplasmic domain of the epidermal growth factor receptor gene in glioblastoma multiforme. Cancer Res 48: 2711-2714, 1988
10. Mishima K, Asai A, Sugiyama A, et al: Increased expression of schwannoma-derived growth factor (SDGF) mRNA in rat tumor cells: involvement of SDGF in the growth promotion of rat gliomas. Int J Cancer 66:352-357, 1996
11. Moroni MC, Willingham MC, Beguinot L: EGF-R antisense RNA blocks expression of the epidermal growth factor receptor and suppresses the transforming phenotype of a human carcinoma cell line. J Biol Chem 267:2714-2722, 1992
12. Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55-63, 1983
13. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning. A Laboratory Manual, ed 2. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989, pp 1.33-1.52
14. Schlegal J, Merdes A, Stumm G, et al: Amplification of the epidermal-growth-factor-receptor gene correlates with different growth behaviour in human glioblastoma. Int J Cancer 56: 72-77, 1994