Antibody response of patients infected with verocytotoxin-producing Escherichia coli to protein antigens encoded on the LEE locus

Journal of Medical Microbiology

Volumn 49, Issue 1, 2000, Pages 97-101

  Jenkins, C ^a   Chart, H ^a   Smith, H R ^a   Hartland, E L ^b   Batchelor, M ^b   Delahay, R M ^b   Dougan, G ^b   Frankel, G ^b  

^a CENTRAL PUBLIC HEALTH LABORATORY   (United Kingdom)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; ESCHERICHIA COLI LIPOPOLYSACCHARIDE; INTIMIN; VEROTOXIN;

ADOLESCENT; ADULT; ANTIBODY RESPONSE; ARTICLE; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; ESCHERICHIA COLI O157; FEMALE; GENE LOCUS; HUMAN; IMMUNOBLOTTING; MALE; NONHUMAN; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRESCHOOL CHILD; PRIORITY JOURNAL; SCHOOL CHILD; SERUM;

EID: 0033957749     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-2615-49-1-97     Document Type: Article

References (16)

1. Dundas S, Todd WTA. Escherichia coli O157 and human disease. Curr Opin Infect Dis 1998; 11: 171-175.
2. Mead PS, Griffin PM. Escherichia coli O157:H7. Lancet 1998; 352: 1207-1212.
3. Novillo AA, Voyer LE, Cravioto R et al. Haemolytic uraemic syndrome associated with faecal cytotoxin and Verocytotoxin neutralizing antibodies. Pediatr Nephrol 1998; 2: 288-290.
4. Scotland SM, Rowe B, Smith HR, Willshaw GA, Gross RJ. Vero cytotoxin-producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. J Med Microbiol 1988; 25: 237-243.
5. Frankel G, Phillips AD, Rosenshine I, Dougan G, Kaper JB, Knutton S. Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements. Mol Microbiol 1998; 30: 911-921.
6. Jerse AE, Gicquelais KG, Kaper JB. Plasmid and chromosomal elements involved in the pathogenesis of attaching and effacing Escherichia coli. Infect Immun 1991; 59: 3869-3875.
7. Frankel G, Candy DCA, Everest P, Dougan G. Characterization of the C-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic Escherichia coli, Citmbacter freundii and Hafnia alvei. Infect Immun 1994; 62: 1835-1842
8. Adu-Bobie J, Trabulsi LR, Carneiro-Sampaio MMS, Dougan G, Frankel G. Identification of immunodominant regions within the C-terminal cell binding domain of intimin α and intimin β from enteropathogenic Escherichia coli. Infect Immun 1998; 66: 5643-5649.
9. Knutton S, Rosenshine I, Rallen MJ et al. A novel EspA-associated surface organeile of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells. EMBO J 1998; 17: 2166-2176.
10. Chart H. Serodiagnosis of infections caused by Escherichia coli O157:H7 and other VTEC. Serodiagn Immunother Infect Dis 1993; 1: 8-12.
11. Chart H, Jenkins C. Salivary antibodies to lipopolysaccharide antigens of Escherichia coli O157. Lancet 1998; 352: 371.
12. Chart H, Jenkins C, Smith HR, Rowe B. Serum antibodies to secreted proteins in patients infected with Escherichia coli O157 and other VTEC. Epidemiol Infect 1998; 120: 239-243.
13. Paton AW, Manning PA, Woodrow MC, Paton JC. Translocated intimin receptors (Tir) of Shiga-toxigenic Escherichia coli isolates belonging to serogroups O26, 0111, and O157 react with sera from patients with hemolytic uremic syndrome and exhibit marked sequence heterogeneity. Infect Immun 1998; 66: 5580-5586.
14. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685.
15. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc Natl Acad Sci USA 1997; 76: 4350-4354.
16. Hartland EL, Batchelor M, Delahay RM et al. Binding of intimin from enteropathogenic Escherichia coli to Tir and to host cells. Mol Microbiol 1999; 32: 151-158.