Minimal compression of ultrathin sections with use of an oscillating diamond knife

Journal of Microscopy

Volumn 197, Issue 1, 2000, Pages 94-100

  Studer, D ^a,b   Gnaegi, H ^c  

^a UNIVERSITY OF BERN   (Switzerland)

^b UNIVERSITY OF LAUSANNE   (Switzerland)

^c Diatome SA   (Switzerland)

Author keywords

Electron microscopy; Oscillating diamond knife; Ultramicrotome; Ultrathin sectioning

Indexed keywords

CERAMIC MATERIALS; ELECTRON MICROSCOPY;

ALTERNATING VOLTAGES; COMPRESSION ARTIFACTS; DIAMOND KNIVES; LOW VOLTAGES; OSCILLATING DIAMOND KNIFE; PIEZOELECTRIC TRANSLATORS; ULTRA-THIN; ULTRAMICROTOME; ULTRATHIN SECTIONING; VOLTAGE SOURCE;

DIAMONDS;

ARTICLE; COMPRESSION; DEVICE; DINOFLAGELLATE; ELECTRON MICROSCOPY; MICROTOME; NONHUMAN; PRIORITY JOURNAL;

DINOPHYCEAE;

EID: 0033956806     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.2000.00638.x     Document Type: Article

References (7)

1. Carlemalm, E., Villiger, W., Hobot, J.A., Acetarin, J.D. & Kellenberger, E. (1985) Low temperature embedding with Lowicryl resins: two new formulations and some applications. J. Microsc. 140, 55-63.
2. Jésior, J.C. (1989) Use of low-angle diamond knives leads to improved ultrastructural preservation of ultrathin sections. Scanning Microsc. Suppl. 3, 147-153.
3. Olbrich, E. (1947) Bändern mit schrägem Messer. Mikroskopie (Wien), 2, 245-250.
4. Reynolds, E.S. (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208-212.
5. Richter, K. (1994) Cutting artefacts on ultrathin cryosections of biological bulk specimens. Micron, 25, 297-308.
6. Sitte, H. & Neumann, K. (1983) Ultramikrotome und apparative Hilfsmittel für die Ultramikrotomie. Methodensammlung der Elektronenmikroskopie 1.1.2 (ed. by G. Schimmel and W. Vogell), pp. 172-175. Wissenschaftliche Verlagsgesellschaft, Stuttgart.
7. Smith, R.E. (1970) Comparative evaluation of two instruments and procedures to cut nonfrozen sections. J. Histochem. Cytochem. 18, 590-591.