Effect of ethidium bromide intercalation on DNA radiosensitivity

International Journal of Radiation Biology

Volumn 76, Issue 1, 2000, Pages 1-9

  Begusova M ^a,b   Spotheim Maurizot, M ^a   Michalik, V ^b   Charlier, M ^a  

^a CENTRE DE BIOPHYSIQUE MOLÉCULAIRE   (France)

^b Department of Radiation Dosimetry Nuclear Physics Institute of the ACSR   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; ETHIDIUM BROMIDE; SCAVENGER; SINGLE STRANDED DNA;

ARTICLE; CHEMICAL MODIFICATION; DNA RESTRICTION; NONHUMAN; PLASMID; PRIORITY JOURNAL; RADIATION PROTECTION; RADIOSENSITIVITY;

EID: 0033956069     PISSN: 09553002     EISSN: None     Source Type: Journal    
DOI: 10.1080/095530000138952     Document Type: Article

References (21)

1. Boles, T. C., White, J. H. and Cozzarelli, N. R., 1990, Structure of plectonemically supercoiled DNA. Journal of Molecular Biology, 213, 931-951.
2. -) in aqueous solution. Journal of Physical and Chemical Reference Data, 17, 513-886.
3. Clendenning, J. B., Naismushin, A. N., Fujimoto, B. S., Stewart, D. W. and Schurr, J. M., 1994, Effect of ethidium binding and superhelix density on the super-coiling free energy and torsion and bending constants of p30δ DNA. Biophysical Chemistry, 52, 191-218.
4. Culard, F., Spotheim-Maurizot, M., Sabattier, R. and Charlier, M., 1994, Radiosensitivity of DNA minicircles. International Journal of Radiation Biology, 65, 651-656.
5. Franchet-Beuzit, J., Spotheim-Maurizot, M., Sabattier, R., Blazy-Baudras, B. and Charlier, M., 1993, Radiolytic footprinting. β rays, γ photons and fast neutrons probe DNA protein interactions. Biochemistry, 32, 2104-2110.
6. Fuller, F., 1982, A family of cloning vectors containing the lac UV5 promoter, Gene, 19, 43-54.
7. Green, N. J. B., Pilling, M. J., Pimblott, S. M. and Clifford, P., 1990, Stochastic modelling of fast kinetics in radiation track, Journal of Physical Chemistry, 94, 251-258.
8. Keller, W., 1975, Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis. Proceedings of the National Academy of Sciences, USA, 72, 4876-4880.
9. Le Pecq, J. B. and Paoletti, C., 1967, A fluorescent complex between Ethidium Bromide and nucleic acids. Physical chemical characterization. Journal of Molecular Biology, 27, 87-106.
10. Miller, J. H., Nelson, J. M., Ye, M., Swenberg, C. E., Speicher, J. M. and Benham, C. J., 1991, Negative supercoiling increases the sensitivity of plasmid DNA to single-strand break induction by X-rays. International Journal of Radiation Biology, 59, 941-949.
11. Milligan, J. R., Arnold, A. D. and Ward, J. F., 1992, The effect of superhelical density on the yield of single-strand breaks in γ-irradiated plasmid DNA. Radiation Research, 132, 69-73.
12. Ozols, A., Price, K. M. and Michael, B. D., 1999, A comparison of the radiosensitivity of relaxed and supercoiled plasmid DNA. International Journal of Radiation Biology, 75, 83-90.
13. Sambrook, J., Fritsch, E. F. and Maniatis, T., 1989, Molecular Cloning: A Laboratory manual, 2nd edn, vol. 1 (New York: Cold Spring Harbor Laboratory Press).
14. Sobell, H. M., Sakore, T. D., Jain, S. C., Banerjee, A., Bhandary, K. K., Reddy, B. S. and Lozansky, E. D., 1983, β-kinked DNA - a structure that gives rise to drug intercalation and DNA breathing and its wider significance in determining the premelting and melting behavior of DNA. Cold Spring Harbor Symposium Quantum Biology, 47, 293-314.
15. Spotheim-Maurizot, M., Franchet, J., Sabattier, R. and Charlier, M., 1991, DNA radiolysis by fast neutrons. II. Oxygen, thiols and ionic strength effects. International Journal of Radiation Biology, 59, 1313-1324.
16. Spotheim-Maurizot, M., Franchet-Beuzit, J., Isabelle, V., Tartier, L. and Charlier, M., 1995, DNA radiolysis. Mapping of the gene regulation domains. Nuclear Instruments and Methods in Physics Research B, 105, 308-313.
17. Swenberg, C. E. and Speicher, J. M., 1995, Neutron and gamma-radiation sensitivity of plasmid DNA of varying superhelical density. Radiation Research, 144, 301-309.
18. Sy, D., Savoye, C., Begusova, M., Michalik, V., Charlier, M. and Spotheim-Maurizot, M., 1997, Sequence-dependent variations of DNA structure modulate radiation-induced strand breakage. International Journal of Radiation Biology, 72, 147-155.
19. Tartier, L., Michalik, V., Spotheim-Maurizot, M., Rahmouni, A. R., Sabattier, R. and Charlier, M., 1994, Radiolytic signature of Z-DNA, Nucleic Acids Research, 22, 5565-5570.
20. Tartier, L., Spotheim-Maurizot, M. and Charlier, M., 1998, Radiosensitivity as support for the structure of a unimolecular DNA quadruplex. International Journal of Radiation Biology, 73, 45-51.
21. Wang, J. C., 1974, The degree of unwinding of the DNA helix by Ethidium I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients. Journal of Molecular Biology, 89, 783-801.