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Volumn 5, Issue 1, 2000, Pages 153-162

The ves multigene family of B. bovis encodes components of rapid antigenic variation at the infected erythrocyte surface

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Indexed keywords

PROTOZOAL PROTEIN;

EID: 0033954897     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(00)80411-6     Document Type: Article
Times cited : (81)

References (54)
  • 1
    • 0033083101 scopus 로고    scopus 로고
    • Antigenic variation in malaria: A 3′ genomic alteration associated with the expression of a P. knowlesi variant antigen
    • Al-Khedery, B., Barnwell, J.W., and Galinski, M.R. (1999). Antigenic variation in malaria: a 3′ genomic alteration associated with the expression of a P. knowlesi variant antigen. Mol. Cell 3, 131-141.
    • (1999) Mol. Cell , vol.3 , pp. 131-141
    • Al-Khedery, B.1    Barnwell, J.W.2    Galinski, M.R.3
  • 2
    • 0028985520 scopus 로고
    • Immune evasion by Babesia bovis and Plasmodium falciparum: Cliff-dwellers of the parasite world
    • Allred, D.R. (1995). Immune evasion by Babesia bovis and Plasmodium falciparum: cliff-dwellers of the parasite world. Parasitol. Today 11, 100-105.
    • (1995) Parasitol. Today , vol.11 , pp. 100-105
    • Allred, D.R.1
  • 3
    • 0031253045 scopus 로고    scopus 로고
    • Immunochemical methods for identification of Babesia bovis antigens expressed on the erythrocyte surface
    • Allred, D.R. (1997). Immunochemical methods for identification of Babesia bovis antigens expressed on the erythrocyte surface. Methods 13, 177-189.
    • (1997) Methods , vol.13 , pp. 177-189
    • Allred, D.R.1
  • 4
    • 0031837563 scopus 로고    scopus 로고
    • Antigenic variation in Babesia bovis: How similar is it to that in Plasmodium falciparum?
    • Allred, D.R. (1998). Antigenic variation in Babesia bovis: how similar is it to that in Plasmodium falciparum? Ann. Trop. Med. Parasitol. 92, 461-472.
    • (1998) Ann. Trop. Med. Parasitol. , vol.92 , pp. 461-472
    • Allred, D.R.1
  • 5
    • 0027225334 scopus 로고
    • Isolate-specific parasite antigens of the Babesia bovis-infected erythrocyte surface
    • Allred, D.R., Hines, S.A., and Ahrens, K.P. (1993). Isolate-specific parasite antigens of the Babesia bovis-infected erythrocyte surface. Mol. Biochem. Parasitol. 60, 121-132.
    • (1993) Mol. Biochem. Parasitol. , vol.60 , pp. 121-132
    • Allred, D.R.1    Hines, S.A.2    Ahrens, K.P.3
  • 6
    • 0028115734 scopus 로고
    • Antigenic variation of parasite-derived antigens on the surface of Babesia bovis-infected erythrocytes
    • Allred, D.R., Cinque, R.M., Lane, T.J., and Ahrens, K.P. (1994). Antigenic variation of parasite-derived antigens on the surface of Babesia bovis-infected erythrocytes. Infect. Immun. 62, 91-98.
    • (1994) Infect. Immun. , vol.62 , pp. 91-98
    • Allred, D.R.1    Cinque, R.M.2    Lane, T.J.3    Ahrens, K.P.4
  • 7
    • 0027399888 scopus 로고
    • The importance of mosaic genes to trypanosome survival
    • Barbet, A.F., and Kamper, S.M. (1993). The importance of mosaic genes to trypanosome survival. Parasitol. Today 9, 63-66.
    • (1993) Parasitol. Today , vol.9 , pp. 63-66
    • Barbet, A.F.1    Kamper, S.M.2
  • 8
    • 0024417163 scopus 로고
    • A human 88-kD membrane glycoprotein(CD36) functions in vitro as a receptor for a cytoadherence ligand on Plasmodium falciparum-infected erythrocytes
    • Barnwell, J.W., Asch, A.S., Nachman, R.L., Yamaya, M., Aikawa, M., and Ingravallo, P. (1989). A human 88-kD membrane glycoprotein(CD36) functions in vitro as a receptor for a cytoadherence ligand on Plasmodium falciparum - infected erythrocytes. J. Clin. Invest. 84, 765-772.
    • (1989) J. Clin. Invest. , vol.84 , pp. 765-772
    • Barnwell, J.W.1    Asch, A.S.2    Nachman, R.L.3    Yamaya, M.4    Aikawa, M.5    Ingravallo, P.6
  • 9
    • 0029052741 scopus 로고
    • Cloning the P. falciparum gene encoding PfEMP1, a malarial variant antigen and adherence receptor on the surface of parasitized human erythrocytes
    • Baruch, D.I., Pasloske, B.L., Singh, H.B., Bi, X., Ma, X.C., Feldman, M., Taraschi, T.F., and Howard, R.J. (1995). Cloning the P. falciparum gene encoding PfEMP1, a malarial variant antigen and adherence receptor on the surface of parasitized human erythrocytes. Cell 82, 77-87.
    • (1995) Cell , vol.82 , pp. 77-87
    • Baruch, D.I.1    Pasloske, B.L.2    Singh, H.B.3    Bi, X.4    Ma, X.C.5    Feldman, M.6    Taraschi, T.F.7    Howard, R.J.8
  • 10
    • 0029918601 scopus 로고    scopus 로고
    • Plasmodium falciparum erythrocyte membrane protein 1 is a parasitized erythrocyte receptor for adherence to CD36, thrombospondin, and intercellular adhesion molecule 1
    • Baruch, D.I., Gormley, J.A., Ma, C., Howard, R.J., and Pasloske, B.L. (1996). Plasmodium falciparum erythrocyte membrane protein 1 is a parasitized erythrocyte receptor for adherence to CD36, thrombospondin, and intercellular adhesion molecule 1. Proc. Natl. Acad. Sci. USA 93, 3497-3502.
    • (1996) Proc. Natl. Acad. Sci. USA , vol.93 , pp. 3497-3502
    • Baruch, D.I.1    Gormley, J.A.2    Ma, C.3    Howard, R.J.4    Pasloske, B.L.5
  • 11
    • 0030735362 scopus 로고    scopus 로고
    • Identification of a region of PfEMP1 that mediates adherence of Plasmodium falciparum infected erythocytes to CD36: Conserved function with variant sequence
    • Baruch, D.I., Ma, X.C., Singh, H.B., Bi, X., Pasloske, B.L., and Howard, R.J. (1997). Identification of a region of PfEMP1 that mediates adherence of Plasmodium falciparum infected erythocytes to CD36: conserved function with variant sequence. Blood 90, 3766-3775.
    • (1997) Blood , vol.90 , pp. 3766-3775
    • Baruch, D.I.1    Ma, X.C.2    Singh, H.B.3    Bi, X.4    Pasloske, B.L.5    Howard, R.J.6
  • 12
    • 0030977377 scopus 로고    scopus 로고
    • Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion
    • Bauer, P., Rolfs, A., Regitz-Zagrosek, V., Hildebrandt, A., and Fleck, E. (1997). Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion. BioTechniques 22, 1128-1132.
    • (1997) BioTechniques , vol.22 , pp. 1128-1132
    • Bauer, P.1    Rolfs, A.2    Regitz-Zagrosek, V.3    Hildebrandt, A.4    Fleck, E.5
  • 14
    • 0008932456 scopus 로고
    • Observations of estivo-autumnal malaria
    • Bignami, A., and Bastianeli, A. (1889). Observations of estivo-autumnal malaria. Riforma Medica 6, 1334-1335.
    • (1889) Riforma Medica , vol.6 , pp. 1334-1335
    • Bignami, A.1    Bastianeli, A.2
  • 16
    • 0030781419 scopus 로고    scopus 로고
    • Long PCR facilitates concise cloning and sequencing with a minimal tiling set of templates
    • Burland, V., and Kusukawa, N. (1997). Long PCR facilitates concise cloning and sequencing with a minimal tiling set of templates. Bio-Techniques 23, 1070-1075.
    • (1997) Bio-Techniques , vol.23 , pp. 1070-1075
    • Burland, V.1    Kusukawa, N.2
  • 18
    • 0027204666 scopus 로고
    • Sequence of a cDNA encoding β-tubulin from Babesia bovis
    • Casu, R.E. (1993). Sequence of a cDNA encoding β-tubulin from Babesia bovis. Mol. Biochem. Parasitol. 59, 339-340.
    • (1993) Mol. Biochem. Parasitol. , vol.59 , pp. 339-340
    • Casu, R.E.1
  • 20
    • 0027389206 scopus 로고
    • Characterisation of a family of multi-copy genes encoding rhoptry protein homologues in Babesia bovis, Babesia ovis and Babesia canis
    • Dalrymple, B.P., Casu, R.E., Peters, J.M., Dimmock, C.M., Gale, K.R., Boese, R., and Wright, l.G. (1993). Characterisation of a family of multi-copy genes encoding rhoptry protein homologues in Babesia bovis, Babesia ovis and Babesia canis. Mol. Biochem. Parasitol. 57, 181-192.
    • (1993) Mol. Biochem. Parasitol. , vol.57 , pp. 181-192
    • Dalrymple, B.P.1    Casu, R.E.2    Peters, J.M.3    Dimmock, C.M.4    Gale, K.R.5    Boese, R.6    Wright, L.G.7
  • 21
    • 0033062625 scopus 로고    scopus 로고
    • Intra-cluster recombination and var transcription switches in the antigenic variation of Plasmodium falciparum
    • Deitsch, K.W., del Pinal, A., and Wellems, T.E. (1999). Intra-cluster recombination and var transcription switches in the antigenic variation of Plasmodium falciparum. Mol. Biochem. Parasitol. 101, 107-116.
    • (1999) Mol. Biochem. Parasitol. , vol.101 , pp. 107-116
    • Deitsch, K.W.1    Del Pinal, A.2    Wellems, T.E.3
  • 22
    • 0030927168 scopus 로고    scopus 로고
    • Expression of var genes located within polymorphic subtelomeric domains of Plasmodium falciparum chromosomes
    • Fischer, K., Horrocks, P., Preub, M., Wiesner, J., Wünsch, S., Camargo, A.A., and Lanzer, M. (1997). Expression of var genes located within polymorphic subtelomeric domains of Plasmodium falciparum chromosomes. Mol. Cell. Biol. 17, 3679-3686.
    • (1997) Mol. Cell. Biol. , vol.17 , pp. 3679-3686
    • Fischer, K.1    Horrocks, P.2    Preub, M.3    Wiesner, J.4    Wünsch, S.5    Camargo, A.A.6    Lanzer, M.7
  • 23
    • 0024212067 scopus 로고
    • Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer
    • Frohman, M.A., Dush, M.K., and Martin, G.R. (1988). Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002.
    • (1988) Proc. Natl. Acad. Sci. USA , vol.85 , pp. 8998-9002
    • Frohman, M.A.1    Dush, M.K.2    Martin, G.R.3
  • 24
  • 25
    • 0028981336 scopus 로고
    • Alternative method to remove antibacterial antibodies from antisera used for screening of expression libraries
    • Gruber, A., and Zingales, B. (1995). Alternative method to remove antibacterial antibodies from antisera used for screening of expression libraries. BioTechniques 19, 28-29.
    • (1995) BioTechniques , vol.19 , pp. 28-29
    • Gruber, A.1    Zingales, B.2
  • 26
    • 0021253525 scopus 로고
    • Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing
    • Henikoff, S. (1984). Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28, 351-359.
    • (1984) Gene , vol.28 , pp. 351-359
    • Henikoff, S.1
  • 27
    • 0031826040 scopus 로고    scopus 로고
    • SOSUI: Classification and secondary structure prediction system for membrane proteins
    • Hirokawa, T., Boon-Chieng, S., and Mitaku, S. (1998). SOSUI: classification and secondary structure prediction system for membrane proteins. Bioinformatics 14, 378-379.
    • (1998) Bioinformatics , vol.14 , pp. 378-379
    • Hirokawa, T.1    Boon-Chieng, S.2    Mitaku, S.3
  • 28
    • 0020787171 scopus 로고
    • Antigenic variation in Plasmodium knowlesi malaria: Identification of the variant antigen on infected erythrocytes
    • Howard, R.J., Barnwell, J.W., and Kao, V. (1983). Antigenic variation in Plasmodium knowlesi malaria: identification of the variant antigen on infected erythrocytes. Proc. Natl. Acad. Sci. USA 80, 4129-4133.
    • (1983) Proc. Natl. Acad. Sci. USA , vol.80 , pp. 4129-4133
    • Howard, R.J.1    Barnwell, J.W.2    Kao, V.3
  • 30
    • 0031455876 scopus 로고    scopus 로고
    • Babesia bovis: Genome size, number of chromosomes and telomeric probe hybridisation
    • Jones, S.H., Lew, A.E., Jorgensen, W.K., and Barker, S.C. (1997). Babesia bovis: genome size, number of chromosomes and telomeric probe hybridisation. Int. J. Parasitol. 27, 1569-1573.
    • (1997) Int. J. Parasitol. , vol.27 , pp. 1569-1573
    • Jones, S.H.1    Lew, A.E.2    Jorgensen, W.K.3    Barker, S.C.4
  • 31
    • 0020475449 scopus 로고
    • A simple method for displaying the hydropathic character of a protein
    • Kyte, J., and Doolittle, R.F. (1982). A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157, 105-132.
    • (1982) J. Mol. Biol. , vol.157 , pp. 105-132
    • Kyte, J.1    Doolittle, R.F.2
  • 32
    • 0015123715 scopus 로고
    • Plasmodium falciparum malaria: Ultrastructure of parasitized erythrocytes in cardiac vessels
    • Luse, S.A., and Miller, L.H. (1971). Plasmodium falciparum malaria: ultrastructure of parasitized erythrocytes in cardiac vessels. Am. J. Trop. Med. Hyg. 20, 655-660.
    • (1971) Am. J. Trop. Med. Hyg. , vol.20 , pp. 655-660
    • Luse, S.A.1    Miller, L.H.2
  • 33
    • 0015841506 scopus 로고
    • Bovine babesiasis: The persistance of immunity to Babesia argentina and B. bigemina in calves (Bos taurus) after naturally acquired infection
    • Mahoney, D.F., Wright, I.G., and Mirre, G.B. (1973). Bovine babesiasis: the persistance of immunity to Babesia argentina and B. bigemina in calves (Bos taurus) after naturally acquired infection. Ann. Trop. Med. Parasitol. 67, 197-203.
    • (1973) Ann. Trop. Med. Parasitol. , vol.67 , pp. 197-203
    • Mahoney, D.F.1    Wright, I.G.2    Mirre, G.B.3
  • 34
    • 0018509514 scopus 로고
    • The immune response of cattle to Babesia bovis (syn. B. Argentina). Studies on the nature and specificity of protection
    • Mahoney, D.F., Kerr, J.D., Goodger, B.V., and Wright, I.G. (1979). The immune response of cattle to Babesia bovis (syn. B. argentina). Studies on the nature and specificity of protection. Int. J. Parasitol. 9, 297-306.
    • (1979) Int. J. Parasitol. , vol.9 , pp. 297-306
    • Mahoney, D.F.1    Kerr, J.D.2    Goodger, B.V.3    Wright, I.G.4
  • 37
    • 0034651882 scopus 로고    scopus 로고
    • Selection of Babesia bovis-infected erythrocytes for adhesion to endothelial cells co-selects for altered variant erythrocyte surface antigen isoforms
    • in press
    • O'Connor, R.M., and Allred, D.R. (2000). Selection of Babesia bovis-infected erythrocytes for adhesion to endothelial cells co-selects for altered variant erythrocyte surface antigen isoforms. J. Immunol., in press.
    • (2000) J. Immunol.
    • O'Connor, R.M.1    Allred, D.R.2
  • 38
    • 0030866037 scopus 로고    scopus 로고
    • Characterization of a variant erythrocyte surface antigen (VESA1) expressed by Babesia bovis during antigenic variation
    • O'Connor, R.M., Lane, T.J., Stroup, S.E., and Allred, D.R. (1997). Characterization of a variant erythrocyte surface antigen (VESA1) expressed by Babesia bovis during antigenic variation. Mol. Biochem. Parasitol. 89, 259-270.
    • (1997) Mol. Biochem. Parasitol. , vol.89 , pp. 259-270
    • O'Connor, R.M.1    Lane, T.J.2    Stroup, S.E.3    Allred, D.R.4
  • 39
    • 0032792686 scopus 로고    scopus 로고
    • Cytoadherence of Babesia bovis-infected erythrocytes to bovine brain capillary endothelial cells provides an in vitro model for sequestration
    • O'Connor, R.M., Long, J.A., and Allred, D.R. (1999). Cytoadherence of Babesia bovis-infected erythrocytes to bovine brain capillary endothelial cells provides an in vitro model for sequestration. Infect. Immun. 67, 3921-3928.
    • (1999) Infect. Immun. , vol.67 , pp. 3921-3928
    • O'Connor, R.M.1    Long, J.A.2    Allred, D.R.3
  • 42
    • 0000934204 scopus 로고
    • Characteristics of the piroplasms Babesia argentina and B. bigemina in the United States
    • Rees, C.W. (1934). Characteristics of the piroplasms Babesia argentina and B. bigemina in the United States. J. Agric. Res. 48, 427-438.
    • (1934) J. Agric. Res. , vol.48 , pp. 427-438
    • Rees, C.W.1
  • 44
    • 0032530397 scopus 로고    scopus 로고
    • Antigenic variation in malaria: In situ switching, relaxed and mutually exclusive transcription of var genes during intra-erythrocytic development in Plasmodium falciparum
    • Scherf, A., Hernandez-Rivas, R., Buffet, P., Bottius, E., Benatar, C., Pouvelle, B., Gysin, J., and Lanzer, M. (1998). Antigenic variation in malaria: in situ switching, relaxed and mutually exclusive transcription of var genes during intra-erythrocytic development in Plasmodium falciparum. EMBO J. 17, 5418-5426.
    • (1998) EMBO J. , vol.17 , pp. 5418-5426
    • Scherf, A.1    Hernandez-Rivas, R.2    Buffet, P.3    Bottius, E.4    Benatar, C.5    Pouvelle, B.6    Gysin, J.7    Lanzer, M.8
  • 45
    • 0031456850 scopus 로고    scopus 로고
    • Classification of introns: U2-type or U12-type
    • Sharp, P.A., and Burge, C.B. (1997). Classification of introns: U2-type or U12-type. Cell 91, 875-879.
    • (1997) Cell , vol.91 , pp. 875-879
    • Sharp, P.A.1    Burge, C.B.2
  • 46
    • 0028982168 scopus 로고
    • Switches in expression of Plasmodium falciparum var genes correlate with changes in antigenic and cytoadherent phenotypes of infected erythrocytes
    • Smith, J.D., Chitnis, C.E., Craig, A.G., Roberts, D.J., Hudson-Taylor, D.E., Peterson, D.S., Pinches, R., Newbold, C.I., and Miller, L.H. (1995). Switches in expression of Plasmodium falciparum var genes correlate with changes in antigenic and cytoadherent phenotypes of infected erythrocytes. Cell 82, 101-110.
    • (1995) Cell , vol.82 , pp. 101-110
    • Smith, J.D.1    Chitnis, C.E.2    Craig, A.G.3    Roberts, D.J.4    Hudson-Taylor, D.E.5    Peterson, D.S.6    Pinches, R.7    Newbold, C.I.8    Miller, L.H.9
  • 47
    • 0032583103 scopus 로고    scopus 로고
    • Analysis of adhesive domains from the A4VAR Plasmodium falciparum erythrocyte membrane protein-1 identifies a CD36 binding domain
    • Smith, J.D., Kyes, S., Craig, A.G., Fagan, T., Hudson-Taylor, D., Miller, L.H., Baruch, D.I., and Newbold, C.I. (1998). Analysis of adhesive domains from the A4VAR Plasmodium falciparum erythrocyte membrane protein-1 identifies a CD36 binding domain. Mol. Biochem. Parasitol. 97, 133-148.
    • (1998) Mol. Biochem. Parasitol. , vol.97 , pp. 133-148
    • Smith, J.D.1    Kyes, S.2    Craig, A.G.3    Fagan, T.4    Hudson-Taylor, D.5    Miller, L.H.6    Baruch, D.I.7    Newbold, C.I.8
  • 48
    • 0031614678 scopus 로고    scopus 로고
    • A hidden markov model for predicting transmembrane helices in protein sequences
    • J. Glasgow, F. Littlejohn, R. Major, D. Lathrop, D. Sankoff, and C. Sensen, eds. (Menlo Park, CA: AAAI Press)
    • Sonnhammer, E.L.L., Von Heijne, G., and Krogh, A. (1998). A hidden Markov model for predicting transmembrane helices in protein sequences. In Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology, J. Glasgow, F. Littlejohn, R. Major, D. Lathrop, D. Sankoff, and C. Sensen, eds. (Menlo Park, CA: AAAI Press), pp. 175-182.
    • (1998) Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology , pp. 175-182
    • Sonnhammer, E.L.L.1    Von Heijne, G.2    Krogh, A.3
  • 49
    • 0029070914 scopus 로고
    • The large diverse gene family var encodes proteins involved in cytoadherence and antigenic variation of plasmodium falciparum-infected erythrocytes
    • Su, X.Z., Heatwole, V.M., Wertheimer, S.P., Guinet, F., Herrfeldt, J.A., Peterson, D.S., Ravetch, J.A., and Wellems, T.E. (1995). The large diverse gene family var encodes proteins involved in cytoadherence and antigenic variation of Plasmodium falciparum-infected erythrocytes. Cell 82, 89-100.
    • (1995) Cell , vol.82 , pp. 89-100
    • Su, X.Z.1    Heatwole, V.M.2    Wertheimer, S.P.3    Guinet, F.4    Herrfeldt, J.A.5    Peterson, D.S.6    Ravetch, J.A.7    Wellems, T.E.8
  • 50
    • 0032104019 scopus 로고    scopus 로고
    • Structure, sequence, and transcriptional analysis of the Babesia bovis rap-1 multigene locus
    • Suarez, C.E., Palmer, G.H., Hötzel, I., and McElwain, T.F. (1998). Structure, sequence, and transcriptional analysis of the Babesia bovis rap-1 multigene locus. Mol. Biochem. Parasitol. 93, 215-224.
    • (1998) Mol. Biochem. Parasitol. , vol.93 , pp. 215-224
    • Suarez, C.E.1    Palmer, G.H.2    Hötzel, I.3    McElwain, T.F.4
  • 52
    • 0015350785 scopus 로고
    • An electron microscopic study of intravascular agglutination in the cerebral cortex due to Babesia argentina infection
    • Wright, I.G. (1972). An electron microscopic study of intravascular agglutination in the cerebral cortex due to Babesia argentina infection. Int. J. Parasitol. 2, 209-215.
    • (1972) Int. J. Parasitol. , vol.2 , pp. 209-215
    • Wright, I.G.1
  • 53
    • 0015866390 scopus 로고
    • Ultrastructural changes in Babesia argentina-infected erythrocytes in kidney capillaries
    • Wright, I.G. (1973). Ultrastructural changes in Babesia argentina-infected erythrocytes in kidney capillaries. J. Parasitol. 59, 735-736.
    • (1973) J. Parasitol. , vol.59 , pp. 735-736
    • Wright, I.G.1
  • 54
    • 0023766882 scopus 로고
    • Immunopathophysiology of Babesia bovis and Plasmodium falciparum infections
    • Wright, I.G., Goodger, B.V., and Clark, I.A. (1988). Immunopathophysiology of Babesia bovis and Plasmodium falciparum infections. Parasitol. Today 4, 214-218.
    • (1988) Parasitol. Today , vol.4 , pp. 214-218
    • Wright, I.G.1    Goodger, B.V.2    Clark, I.A.3


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