Recovery of protein kinases from renatured SDS-polyacrylamide gels for biochemical studies

BioTechniques

Volumn 28, Issue 2, 2000, Pages 232-238

  Wang, Hwa Chain R ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CELL CULTURE; CHICK EMBRYO; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME ISOLATION; NONHUMAN; PROTEIN PHOSPHORYLATION;

ANIMALIA;

EID: 0033951705     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00282bm09     Document Type: Article

References (16)

1. Boyle, W.J., P. van der Geer and T. Hunter. 1991. Phosphopeptide mapping and phospho-amino acid analysis by two-dimensional separation on thin layer cellulose plates. Methods Enzymol. 201:110-149.
2. Bryant, D. and J.T. Parsons. 1982. Site-directed mutagenesis of the src gene of Rous sarcoma virus: Construction and characterization of a deletion mutant temperature sensitive for transformation. J. Virol. 44:683-691.
3. Chen, Y-R., C.F. Meyer and T-H. Tan. 1996. Persistent activation of c-Jun N-terminal kinase 1 (JNK1) in γ radiation-induced apoptosis. J. Biol.Chem. 277:631-634.
4. Creasy, C.L. and J. Chernoff. 1995. Cloning and characterization of a member of the MST subfamily of Ste20-like kinases. Gene 167:303-306.
5. Creasy, C.L. and J. Chernoff. 1995. Cloning and characterization of a human protein kinase with homology to Ste20. J. Biol. Chem. 270:21695-21700.
6. Dupont, H, S. Audigier and S. Chevalier. 1996. Screening of protein tyrosine kinases activated during neural induction in Xenopus. Anal. Biochem. 237:42-48.
7. Durocher, Y., A. Chapdelaine and S. Chevalier. 1992. Identification of cytosolic protein tyrosine kinases of human prostate by renaturation after SDS/PAGE. Biochem. J. 254:653-658.
8. Kameshita, I. and H. Fujisawa. 1989. A sensitive method for detection of calmodulin-dependent protein kinase II activity in sodium dodecyl sulfate-polyacrylamide gel. Anal. Biochem. 183:139-143.
9. Leevers, S.J. and C.J. Marshall. 1992. Activation of extracellular signal-regulated kinase, Erk2, by p21ras oncoprotein. EMBO J. 11:569-574.
10. Nguyen, L.T., Y. Durocher, A. Chapdelaine and S. Chevalier. 1992. The major form of protein tyrosine kinase in the dog prostate is expressed by a 50 kDa polypeptide. FEBS Lett. 296:215-218.
11. Rajgolikar, G., K.K. Chan and H-C.R. Wang. 1998. Effects of a novel antitumor depsipeptide, FR901228, on human breast cancer cells. Breast Cancer Res. Treat. 57:29-38.
12. Ray, L.B. and T.W. Sturgill. Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro. Proc. Natl. Acad. Sci. USA 84:1502-1506.
13. Reynolds, A.B., J. Vila, T.J. Lansing, W.M. Potts, M.J. Weber and J.T. Parsons. 1987. Activation of the oncogenic potential of the avian cellular src protein by specific structural alteration of the carboxy terminus. EMBO J. 6:2359-2364.
14. Rossomando, A.J., D.M. Payne, M.J. Weber and T.W. Sturgill. 1989. Evidence that pp42, a major tyrosine kinase target protein, is a mitogen-activated serine/threonine protein kinase. Proc. Natl. Acad. Sci. USA 86:6940-6943.
15. Taylor, L.K., H-C.R. Wang and R.L. Erikson. 1996. Newly identified stress-responsive protein kinases, Krs-1 and Krs-2. Proc. Natl. Acad. Sci. USA 95:10099-10104.
16. Wang, H-C.R. and R.L. Erikson. 1992 Activation of protein serine/threonine kinases p42, p63, and p87 in Rous sarcoma virustransformed cells: signal transduction/transformation-dependent MBP kinases. Mol. Biol. Cell 3:1329-1337.