Localisation of a 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the mitochondrial matrix of Trypanosoma brucei procyclics

Zeitschrift fur Naturforschung - Section C Journal of Biosciences

Volumn 55, Issue 5-6, 2000, Pages 473-477

  Heise, Norton ^a,b   Opperdoes, Fred R ^a  

^a DE DUVE INSTITUTE   (Belgium)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

3 Hydroxy 3 methylglutaryl coenzyme A Reductase; Glycosomes; Mitochondrion; Trypanosoma brucei

Indexed keywords

BIOCHEMICAL PATHWAY; CELL FRACTIONATION; CENTRIFUGATION; CHOLESTEROL; DIGITONIN; ENDOPLASMIC RETICULUM; ENZYME LOCALIZATION; ERGOSTEROL; GLYCOSOME; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; LEUCINE; LIPOGENESIS; MITOCHONDRION; THREONINE;

LEISHMANIA; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI;

EID: 0033949781     PISSN: 09395075     EISSN: None     Source Type: Journal    
DOI: 10.1515/znc-2000-5-626     Document Type: Article

References (26)

1. Brun R. and Schönenberger M. (1979), Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Acta Trop. 36, 289-292.
2. Concepción J. L., González-Pacanowska D. and Urbina J. A. (1998), 3-Hydroxy-3-methylglutaryl-CoA reductase in Trypanosoma (Schizotrypanum) cruzi: subcellular localization and kinetic properties. Archiv. Biochem. Biophys. 352, 114-120.
3. Coppens I., Bastin P., Levade T. and Courtoy P. (1995), Activity, pharmacological inhibition and biological regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in Trypanosoma brucei. Mol. Biochem. Parasitol. 69, 29-40.
4. Engfelt W. H., Shackelford J. E., Aboushadi N., Jessani N., Masuda K., Paton V. G., Keller G. A. and Krisans S. K. (1997), Characterization of UT2 cells. The induction of peroxisomal 3-hydroxy 3-methylglutaryl coenzyme A reductase. J. Biol. Chem. 272, 24579-24587.
5. Fujiwara Y., Asogawa M. and Nakai K. (1997), Prediction of mitochondrial targeting signals using hidden Markov models. Genome Informatics Workshop, Genome Informatics, GIW 97, 53-60.
6. Gbenle G. O., Opperdoes F. R. and Van Roy, J. (1986), Localization of 3′-nucleotidase and calcium-dependent endoribonuclease in the plasma-membrane of Trypanosoma brucei. Acta Tropica 43, 295-305.
7. Ginger M. L., Chance M. L. and Goad L. J. (1996), Carbon sources for fatty acid and sterol biosynthesis in Leishmania species. Biochem. Soc. Trans. 24, 434S.
8. Goad L. J., Holz G. G.Jr. and Beach D. H. (1984), Sterols of Leishmania species. Implications for biosynthesis. Mol. Biochem. Parasitol. 10, 161-170.
9. Gonzalez Pacanowska D., Peña-Diaz J., Montalvetti A., Hurtado-Guerrero R. and Ruiz-Pérez L. M. (1999), 3-Hydroxy-3-methylglutaryl coenzyme A reductase in trypanosomatids. Mem. Inst. Oswaldo Cruz 94, 18.
10. Heise N. and Opperdoes F. R. (1999). Purification, localisation and characterization of glucose-6-phosphate dehydrogenase of Trypanosoma brucei. Mol. Biochem. Parasitol. 99, 21-32.
11. Häusler T., Stierhof Y. D., Blattner J. and Clayton C. (1997), Conservation of mitochondrial targeting sequence function in mitochondrial and hydrogenosomal proteins from the early-branching eukaryotes Crithidia, Trypanosoma and Trichomonas. Eur. J. Cell Biol. 73, 240-251.
12. Keller G. A., Pazirandeh M. and Krisan S. (1986), 3-Hydroxy-3-methylglutaryl coenzyme A reductase localization in rat liver peroxisomes and microsomes of control and choletyramine-treated animals: quantitative biochemical and immunoelectron microscopical analyses. J. Cell Biol. 103, 875-886.
13. Klein R. A. and Linstead D. J. (1976) Threonine as preferred source of 2-carbon units for lipid synthesis in Trypanosoma brucei. Biochem. Soc. Trans. 4, 48-50.
14. Linstead D. J., Klein R. A. and Cross G. A. M. (1986), Threonine catabolism in Trypanosoma brucei. J. Gen. Microbiol. 101, 243-251.
15. Misset O. and Opperdoes F. R. (1984), Simultaneous purification of hexokinase, class-I fructose-bisphosphate aldolase, triosephosphate isomerase and phosphoglycerate kinase from Trypanosoma brucei. Eur. J. Biochem. 144, 475-483.
16. Olender E. H. and Simoni R. D. (1992), The intracellular targeting and membrane topology of 3-Hydroxy-3-methylglularyl-CoA reductase. J. Biol. Chem. 267, 4223-4235.
17. Opperdoes F. R., Markos A. and Steiger R. F. (1981), Localization of malate dehydrogenase, adenylate kinase and glycolytic enzymes in glycosomes and the threonine pathway in the mitochondria of cultured procyclic trypomastigotes of Trypanosoma brucei. Mol. Biochem. Parasitol. 4, 291-309.
18. Opperdoes F. R. (1987), Compartmentation of carbohydrate metabolism in trypanosomes. Annu. Rev. Microbiol. 41, 127-151.
19. Patel T. B. and Clark J. B. (1981), Rat brain 3-hydroxy-3-methylglutaryl coenzyme A reductase: subcellular distribution and cold lability. J. Neurochem. 36, 1281-1284.
20. Peña Diaz J., Montalvetti A., Camacho A., Gallego C., Ruiz Perez L. M. and González Pacanowska D. (1997), A soluble 3-hydroxy-3-methylglutaryl-CoA reductase in the protozoan Trypanosoma cruzi. Biochem. J. 324, 619-626.
21. Pignataro O. P., Radicella J. P., Calvo J. C. and Charreau E. H. (1983), Mitochondrial biosynthesis of cholesterol in Leydig cells from rat testis. Mol. Cell. Endocrinol. 33, 53-67.
22. Shapiro D. J., Nordstrom J. L., Mitschlen J. J., Rodwell W. and Schimke R. T. (1974), Micro assay for 3-Hydroxy-3-methylglutaryl-CoA reductase in rat liver and in L-cell fibroblasts. Biochim. Biophys. Acta 370, 369-377.
23. Steiger R. F., Opperdoes F. R. and Bontemps J. (1980), Subcellular fractionation of Trypanosoma brucei bloodstream forms with special reference to hydrolases. Eur. J. Biochem. 105, 163-175.
24. Stein S., Bohlen P., Stone J., Dairman W. and Udenfriend S. (1973), Amino-acid analysis with fluorescamine at the picomole level. Arch. Biochem. Biophys. 155, 203-212.
25. Urbina J. A. (1997), Lipid biosynthesis pathways as chemotherapeutic targets in kinetoplastid parasites. Parasitol. 114Suppl, S91-99.
26. Venkatesan S. and Ormerod W. E. (1976), Lipid content of the slender and stumpy forms of Trypanosoma brucei rhodesiense: a comparative study. Comp. Biochem. Physiol. 53B, 481-487.