Sequestered actin in chick embryo fibroblasts

Molecular and Cellular Biochemistry

Volumn 209, Issue 1-2, 2000, Pages 63-67

  Nagamalleswari, K ^a   Safer, Daniel ^b  

^a GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^b NONE

Author keywords

Actin; ADF; Antibody; Fibroblast; Gelsolin; Polymerization; Profilin; T 4

Indexed keywords

ACTIN; ACTIN BINDING PROTEIN; THYMOSIN BETA4;

ARTICLE; CELL PROLIFERATION; FIBROBLAST; NONHUMAN; POLYMERIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN SECRETION;

EID: 0033949064     PISSN: 03008177     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1007025602110     Document Type: Article

References (30)

1. Theriot JA, Mitchison TJ: Actin microfilament dynamics in locomoting cells. Nature 352: 126-131, 1991
2. Symons MH, Mitchison TJ: Control of actin polymerization in live and permeabilized fibroblasts. J Cell Biol 114: 503-513, 1991
3. Sanger JM, Dome JS, Hock RS, Mittal B, Sanger JW: Occurrence of fibers and their association with talin in the cleavage furrows of PtK2 cells. Cell Motil Cytoskeleton 27: 26-40, 1994
4. Bray D, Thomas C: Unpolymerized actin in fibroblasts and brain. J Mol Biol 105: 527-544, 1976
5. Bamburg JR, Bray D: Distribution and cellular localization of actin depolymerizing factor. J Cell Biol 105: 2817-2825, 1987
6. Buss F, Temm-Grove C, Henning S, Jockusch BM: Distribution of profilin in fibroblasts correlates with the presence of highly dynamic actin filaments. Cell Motil Cytoskeleton 22: 51-61, 1992
7. Goodall GJ, Morgan JI, Horecker BL: Thymosin beta 4 in cultured mammalian cell lines. Arch Biochem Biophys 221: 598-601, 1983
8. Safer D, Devineni N: Mol Biol Cell 4: 383a, 1993
9. Yin HL, Stossel TP: Control of cytoplasmic actin gel-sol transformation by gelsolin, a calcium dependent regulatory protein. Nature 281: 583-586, 1979
10. Harris HE, Weeds AG: Plasma actin depolymerizing factor has both calcium-dependent and calcium-independent effects on actin. Biochem 22: 2728-2741, 1983
11. Kurth M, Bryan J: Platelet activation induces the formation of a stable gelsolin-actin complex from monomeric gelsolin. J Biol Chem 259: 7473-7479, 1984
12. 4 with muscle and platelet actin: Implications for actin sequestration in resting platelets. Biochem 31: 6179-6185, 1992
13. Murray JM, Weber A, Knox MK: Myosin subfragment 1 binding to relaxed actin filaments and steric model of relaxation. Biochem 20: 641-649, 1981
14. Cassimeris L, Safer D, Nachmias VT, Zigmond SH: Thymosin beta 4 sequesters the majority of G-actin in resting human polymorphonuclear leukocytes. J Cell Biol 119: 1261-1270, 1992
15. Kaiser DA, Goldschmidt-Clermont PJ, Levine BA, Pollard TD: Characterization of renatured profilin purified by urea elution from poly-L-proline agarose columns. Cell Motil Cytoskeleton 14: 251-262, 1989
16. Janmey PA: Polyproline affinity method for purification of platelet profilin and modification with pyrene-maleimide. Meth Enzymol 196: 92-99, 1991
17. Nachmias VT, Cassimeris L, Golla R, Safer D: Thymosin beta 4 in activated platelets. Eur J Cell Biol 61: 314-320, 1993
18. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olsen BJ, Klenk DC: Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85, 1985
19. Blikstad I, Markey F, Carlsson L, Persson T, Lindberg U: Selective assay of monomeric and filamentous actin in cell extracts using inhibition of deoxyribonuclease I. Cell 15: 935-943, 1978
20. Safer D: An electrophoretic procedure for detecting proteins that bind actin monomers. Anal Biochem 178: 32-37, 1989
21. 4. Nature 227: 680-685, 1970
22. Towbin H, Staehlin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354, 1979
23. Safer D, Nachmias VT, Golla R: Isolation of 5-kilodalton actin sequestering peptide from human blood platelets. Proc Natl Acad Sci USA 87: 2536-2540, 1990
24. Goldschmidt-Clermont PJ, Furman MI, Wachsstock D, Safer D, Nachmias VT, Pollard TD: The control of actin nucleotide exchange by thymosin beta 4 and profilin. A potential regulatory mechanism for actin polymerization in cells. Mol Biol Cell 3: 1015-1024, 1992
25. Machesky LM, Atkinson SJ, Ampe C, Vandekerckhove J, Pollard TD: Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose. J Cell Biol 127: 107-115, 1994
26. Gieselmann R, Kwiatkowski DJ, Janmey PA, Witke W: Distinct biochemical characteristics of the two human profilin isoforms. Eur J Biochem 229: 621-628, 1995
27. Lambrechts A, Van Damme J, Goethals M, Vandekerckhove J, Ampe C: Purification and characterization of bovine profilin II, actin, poly (L-proline) and inositolphospholipid binding. Eur J Biochem 230: 281-286, 1995
28. Devineni N, Minamide LS, Niu M, Safer D, Verma R, Bamburg JR, Nachmias VT: A quantitative analysis of G-actin binding proteins and the G-actin pool in developing chick brain. Brain Res 823: 129-140, 1999
29. Nagaoka R, Minami N, Hayakawa K, Abe H, Obinata T: Quantitative analysis of low molecular weight G-actin binding proteins, cofilin, ADF and profilin, expressed in developing and degenerating chicken skeletal muscles. J Muscle Res Cell Motil 17: 463-473, 1996
30. Cooper JA, Loftus DA, Frieden C, Bryan J, Elson EL: Localization and mobility of gelsolin in cells. J Cell Biol 106: 1229-1240, 1988