Detection of heat shock protein-70 from Trichinella spiralis larvae using a modification of the routine western blotting procedure

Journal of Parasitology

Volumn 86, Issue 3, 2000, Pages 637-639

  Martinez, J ^b   Perez Serrano, J ^b   Bernadina, W E ^a   Rodriguez Caabeiro, F ^b  

^a UTRECHT UNIVERSITY   (Netherlands)

^b UNIVERSITY OF ALCALÁ   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

HEAT SHOCK PROTEIN 70; MONOCLONAL ANTIBODY;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; EMBRYO; HOST PARASITE INTERACTION; LARVA; MOLECULAR WEIGHT; NONHUMAN; PROTEIN DETERMINATION; PROTEIN EXPRESSION; TRICHINELLA SPIRALIS; WESTERN BLOTTING;

ANIMALIA; NEMATODA; TRICHINELLA SPIRALIS;

EID: 0033948173     PISSN: 00223395     EISSN: None     Source Type: Journal    
DOI: 10.1645/0022-3395(2000)086[0637:dohspf]2.0.co;2     Document Type: Article

References (14)

1. ALLEGRETTI, S., C. HAMBOURG, V. T. HUYNH, AND J. DUPOUY-CAMET. 1997. An HSP60-63 homologue is constitutively expressed in infective larvae of Trichinella spiralis. Parasite 4: 283-285.
2. ANDERS, R. F., R. J. HOWARD, AND G. F. MITCHELL. 1982. Parasite antigens and methods of analysis. In Immunology of parasite infections, S. Cohen and K. S. Warren (eds.). Blackwell, Oxford, U.K., p. 29-73.
3. BOZNER, P. 1997. Immunological detection and subcellulur localization of HSP70 and HSP60 homologs in Trichomonas vaginalis. Journal of Parasitology 83: 224-229.
4. ERNANI, F. P., AND J. M. TEALE. 1993. Release of stress proteins from Mesocestoides corti is a brefelding a-inhibitable process: Evidence for active export of stress proteins. Infection and Immunity 6: 2596-2601.
5. FEIGE, U., AND B. S. POLLA. 1994. Heat shock proteins: The hsp70 family (hsp70) - a multi-gene, multi-structure, multi-function family with potential clinical applications. Experientia 11/12: 979-986.
6. KO, R. C., AND L. FAN. 1996. Heat shock response of Trichinella spiralis and T. pseudospiralis. Parasitology 112: 89-95.
7. LAEMMLI, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
8. MARTINEZ, J., J. PEREZ-SERRANO, W. BERNADINA, AND F. RODRIGUEZ-CAABEIRO. 1999a. Influence of parasitation by Trichinella spiralis on the levels of heat shock proteins in rat liver and muscle. Parasitology 118: 201-209.
9. _, _, _, AND F. _. 1999b. In vitro stress response to elevated temperature, hydrogen peroxide and mebendazole in Trichinella spiralis muscle larvae. International Journal for Parasitology (in press).
10. PLUSKAL, M. G., M. B. PRZEKOP, M. R. KOVANIAN, C. VECOLI, AND D. A. HICKS. 1986. Immobilon PVDF membrane: A new membrane substrate for western blotting of proteins. Biotechniques 4: 272-282.
11. TOWBIN, P., T. STAEHLIN, AND J. GORDON. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proceedings of the National Academy Science USA 76: 4350-4354.
12. VAN LEEUWEN, M. A. W. 1995. Heat-shock and stress response of the parasitic nernatode Haemonchus contortus. Parasitology Research 81: 706-709.
13. VON BRAND, T., P. WEISTEIN, B. MEHLAN, AND E. WEINBACH. 1952. Observation on the metabolism of bacteria free larvae of Trichinella spiralis. Experimental Parasitology 1: 245-255.
14. WALLACE, G. R., A. E. BALL, J. MACFARLANE, S. H. EL SAFI, M. A. MILES, AND J. M. KELLY. 1992. Mapping of a visceral leishmaniasis-specific immunodominant B-cell epitope of Leishmania donovani Hsp70. Infection and Immunity 60: 2688-2693.