Semiquantitative immunoblots of membrane protein-epidermal growth factor

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 15, Issue 1, 2000, Pages 65-67

  Wong, Richard Wing Chuen ^a   Chan, Siu Yuen ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

EGF; Immunoblot; Protein extraction method

Indexed keywords

EPIDERMAL GROWTH FACTOR; MEMBRANE PROTEIN;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; IMMUNOBLOTTING; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION;

EID: 0033945619     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:15:1:65     Document Type: Article

References (7)

1. 1. Wong, R.W.C. (1900) A simple and rapid method for isolating myelin basic protein. Mol. Biotechnol. 13, 17-19.
2. 2. Ausubel, F.M., Brent, R., Kingston, R.E.,Moore, D.D., Seidman, J.G., Smith, J.A., et al. (1997) In Current Protocols in Molecular Biology., Vol. 2, Wiley, New York.
3. 3. Cohen, S. (1962) Isolation of a mouse sub-maxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J. Biol. Chem. 237, 1555-1562.
4. 4. Bell, G.I., Fong, N.M., Stempien, M.M., Wormsted, M.A., Caput, D., Ku, L.L., et al (1986) Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization. Nucleic Acids Res 14, 8427-8446.
5. 5. Kwan, R.W. P., Wong, R.W. C., and Chan, S.Y. (1999) Expression of full length or truncated epidermal growth factor precursor transforms NIH3T3 fibroblasts. Int. J. Oncol. 15, 281-284.
6. 6. Breyer, J.A., and Cohen, S. (1990) The epidermal growth factor precursor isolated from murine kidney membranes. J. Biol. Chem. 265, 16564-16570.
7. 7. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature (London) 227, 680-685.