Integration and excision of a Bacteroides conjugative transposon, CTnDOT

Journal of Bacteriology

Volumn 182, Issue 14, 2000, Pages 4035-4043

  Cheng, Qi ^a   Paszkiet, Brian J ^a   Shoemaker, Nadja B ^a   Gardner, Jeffrey F ^a   Salyers, Abigail A ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TETRACYCLINE;

ARTICLE; BACTERIUM CONJUGATION; BACTEROIDES; DNA FLANKING REGION; EXCISION REPAIR; GENE CONTROL; GENE DISRUPTION; GENE INSERTION; GENE LOCATION; GENE SEQUENCE; GENE TRANSFER; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; TRANSPOSON;

AMINO ACID SEQUENCE; BACTEROIDES; CLONING, MOLECULAR; CONJUGATION, GENETIC; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; DNA, CIRCULAR; GENES, BACTERIAL; INTEGRASES; MOLECULAR SEQUENCE DATA; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); BACTEROIDES; NEGIBACTERIA;

EID: 0033943003     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.14.4035-4043.2000     Document Type: Article

References (34)

1. Altschul, S. F., T. L. Madden, A. A. Schaffler, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped Blast and Psi-Blast: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Bedzyk, L. A., N. B. Shoemaker, K. E. Young, and A. A. Salyers. 1992. Insertion and excision of Bacteroides conjugative chromosomal elements. J. Bacteriol. 174:166-172.
3. Caparon, M. G., and J. R. Scott. 1989. Excision and insertion of the conjugal transposon Tn916 involves a novel recombination mechanism. Cell 59:1027-1034.
4. Celli, J., and P. Trieu-Cuot. 1998. Circularization of Tn916 is required for expression of the transposon-encoded transfer functions: characterization of long tetracycline-inducible transcripts reading through the attachment site. Mol. Microbiol. 28:103-117.
5. Clewell, D. B., and S. E. Flannagan. 1993. The conjugative transposons of gram-positive bacteria, p. 369-393. In D. B. Clewell (ed.). Bacterial conjugation. Plenum Press, New York, N.Y.
6. Feldhaus, M. J., V. Hwa, Q. Cheng, and A. A. Salyers. 1991. Use of an Escherichia coli β-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters. J. Bacteriol. 173:4540-4543.
7. Franke, A. E., and D. B. Clewell. 1981. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid. J. Bacteriol. 145:494-502.
8. Hecht, D. W., J. S. Thompson, and M. H. Malamy. 1989. Characterization of the termini and transposition products of Tn4399, a conjugal mobilizing transposon of Bacteroides fragilis. Proc. Natl. Acad. Sci. USA 86:5340-5344.
9. Holdeman, L. V., and W. E. C. Moore. 1975. Anaerobe laboratory manual. 4th ed. Virginia Polytechnic Institute and State University, Blacksburg, Va.
10. Jia, Y. H., and G. Churchward. 1999. Interactions of the integrase protein of the conjugative transposon Tn916 with its specific DNA binding sites. J. Bacteriol. 181:6114-6223.
11. Li, L. Y., N. B. Shoemaker, and A. A. Salyers. 1995. Location and characteristics of the transfer region of a Bacteroides conjugative transposon and regulation of transfer genes. J. Bacteriol. 177:4992-4999.
12. Manganelli, R., S. Ricci, and G. Pozzi. 1997. The joint of Tn916 circular intermediates is a homoduplex in Enterococcus faecalis. Plasmid 38:71-78.
13. Metcalf, W. W., W. Jiang, and B. L. Wanner. 1994. Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6Kv origin plasmids at different copy numbers. Gene 138:1-7.
14. Nunes-Düby, S. E., H. J. Kwon, R. S. Tirumalai, T. Ellenberger, and A. Landy. 1998. Similarities and differences among 105 members of the Int family of site-specific recombinases. Nucleic Acids Res. 26:391-406.
15. Rudy, C., K. L. Taylor, D. Hinerfeld, J. R. Scott, and G. Churchward. 1997. Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916. Nucleic Acids Res. 25:4061-4066.
16. Saito, H., and K. I. Miura. 1963. Preparation of transforming deoxy-ribonucleic acid by phenol treatment. Biochim. Biophys. Acta 72:619-629.
17. Salyers, A. A., and N. B. Shoemaker. 1995. Conjugative transposons: the force behind the spread of antibiotic resistance genes among Bacteroides clinical isolates. Anaerobe 1:143-150.
18. Salyers, A. A., N. B. Shoemaker, and A. M. Stevens. 1995. Tetracycline regulation of conjugal transfer genes, p. 393-400. In 5. A. Hoch and T. J. Silhavy (ed.), Two-component signal transduction. American Society for Microbiology, Washington. D.C.
19. Salyers, A. A., N. B. Shoemaker, A. M. Stevens, and L. Y. Li. 1995. Conjugative transposons: an unusual and diverse set of integrated gene transfer elements. Microbiol. Rev. 59:579-590.
20. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Press, Cold Spring Harbor, N.Y.
21. Scott, J. R., F. Bringel, D. Marra, G. Van Alstine, and C. K. Rudy. 1994. Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate. Mol. Microbiol. 11:1099-1108.
22. Scott, J. R., and G. G. Churchward. 1995. Conjugative transposition. Annu. Rev. Microbiol. 49:367-397.
23. Shoemaker, N. B., R. D. Barber, and A. A. Salvers. 1989. Cloning and characterization of a Bacteroides conjugal tetracycline-erythromycin resistance element by using a shuttle cosmid vector. J. Bacteriol. 171:1294-1302.
24. Shoemaker, N. B., C. Getty, E. P. Guthrie, and A. A. Salyers. 1986. Regions in Bacteroides plasmids pBFTM10 and pB8-51 that allow Escherichia coli-Bacteroides shuttle vectors to be mobilized by IncP plasmids and by a conjugative Bacteroides tetracycline resistance element. J. Bacteriol. 166:959-965.
25. Shoemaker, N. B., and A. A. Salyers. 1990. A cryptic 65-kilobase-pair transposonlike element isolated from Bacteroides uniformis has homology with Bacteroides conjugal tetracycline resistance elements. J. Bacteriol. 172:1694-1702.
26. Shoemaker, N. B., and A. A. Salyers. 1988. Tetracycline-dependent appearance of plasmidlike forms in Bacteroides uniformis 0061 mediated by conjugal Bacteroides tetracycline resistance elements. J. Bacteriol. 170:1651-1657.
27. Shoemaker, N. B., G. R. Wang, and A. A. Salyers. 1996. The Bacteroides mobilizable insertion element. NBU1, integrates into the 3′ end of a Leu-1RNA gene and has an integrase that is a member of the lambda integrase family. J. Bacteriol. 178:3594-3600.
28. Simon, R., U. Priefer, and A. Puhler. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1:784-791.
29. Stevens, A. M., N. B. Shoemaker, L. Y. Li, and A. A. Salyers. 1993. Tetracycline regulation of genes on Bacteroides conjugative transposons. J. Bacteriol. 175:6134-6141.
30. Stevens, A. M., N. B. Shoemaker, and A. A. Salyers. 1990. The region of a Bacteroides conjugal chromosomal tetracycline resistance element which is responsible for production of plasmidlike forms from unlinked chromosomal DNA might also be involved in transfer of the element. J. Bacteriol. 172: 4271-4279.
31. Tribble, G. D., A. C. Parker, and C. J. Smith. 1997. The Bacteroides mobilizable transposon Tn4555 integrates by a site-specific recombination mechanism similar to that of the gram-positive bacterial element Tn916. J. Bacteriol. 179:2731-2739.
32. Tribble, G. D., A. C. Parker, and C. J. Smith. 1999. Genetic structure and transcriptional analysis of a mobilizable, antibiotic resistance transposon from Bacteroides. Plasmid 42:1-12.
33. Vedantam, G., T. J. Novicki, and D. W. Hecht. 1999. Bacteroides fragilis transfer factor Tn5520: the smallest bacterial mobilizable transposon containing single integrase and mobilization genes that function in Escherichia coli. J. Bacteriol. 181:2564-2571.
34. Wang, J., N. Shoemaker, G.-R. Wang, and A. A. Salyers. 2000. Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene. J. Bacteriol. 182:3559-3571.