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Volumn 16, Issue 3, 2000, Pages 249-251

A mutant subtilisin E with enhanced thermostability

Author keywords

Site directed mutagenesis; Subtilisin E; Thermostability

Indexed keywords

AMIDASE; AQUEOUS SOLUTION; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME SUBSTRATE; MUTANT PROTEIN; N,N DIMETHYLFORMAMIDE; SUBTILISIN; THERMOSTABILITY;

EID: 0033939982     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008959825832     Document Type: Article
Times cited : (9)

References (12)
  • 2
    • 0026334373 scopus 로고
    • Enzyme engineering for nonaqueous solvents: Random mutagenesis to enhance activity of subtilisin E in polar organic media
    • Chen, K. & Arnold, F.H. 1991 Enzyme engineering for nonaqueous solvents: random mutagenesis to enhance activity of subtilisin E in polar organic media. Biotechnology 9, 1073-1077.
    • (1991) Biotechnology , vol.9 , pp. 1073-1077
    • Chen, K.1    Arnold, F.H.2
  • 3
    • 0021830125 scopus 로고
    • Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation
    • Estell, D.A., Graycar, T.P. & Wells, J.A. 1985 Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation. Journal of Biological Chemistry 260, 6518-6521.
    • (1985) Journal of Biological Chemistry , vol.260 , pp. 6518-6521
    • Estell, D.A.1    Graycar, T.P.2    Wells, J.A.3
  • 4
    • 0000500110 scopus 로고
    • The construction shuttle vectors of Bacillus subtilis-Escherichia coli
    • Guo, X.H., Xiong, Z., Zhou, M. & Jia, S.F. 1991 The construction shuttle vectors of Bacillus subtilis-Escherichia coli. Chinese Journal of Biotechnology 7, 224-229.
    • (1991) Chinese Journal of Biotechnology , vol.7 , pp. 224-229
    • Guo, X.H.1    Xiong, Z.2    Zhou, M.3    Jia, S.F.4
  • 5
    • 0021193861 scopus 로고
    • Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases
    • Kawamura, H. & Doi, R.H. 1984 Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases. Journal of Bacteriology 160, 442-444.
    • (1984) Journal of Bacteriology , vol.160 , pp. 442-444
    • Kawamura, H.1    Doi, R.H.2
  • 7
    • 0024962392 scopus 로고
    • Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding
    • Pantiliano, M.W., Whitlow, M., Wood, J.F., Dodd, S.W., Hardman, K.D., Rollence, M.L. & Bryan, P.N. 1989 Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding. Biochemistry 28, 7205-7213.
    • (1989) Biochemistry , vol.28 , pp. 7205-7213
    • Pantiliano, M.W.1    Whitlow, M.2    Wood, J.F.3    Dodd, S.W.4    Hardman, K.D.5    Rollence, M.L.6    Bryan, P.N.7
  • 9
    • 0026551274 scopus 로고
    • The effect of amino acid deletion in subtilisin E, based on structural comparison with a microbial alkaline elastase, on its substrate specificity and catalysis
    • Takagi, H., Arafuka, S., Inouye, M. & Yamasaki, M. 1992 The effect of amino acid deletion in subtilisin E, based on structural comparison with a microbial alkaline elastase, on its substrate specificity and catalysis. Journal of Biochemistry 111, 584-588.
    • (1992) Journal of Biochemistry , vol.111 , pp. 584-588
    • Takagi, H.1    Arafuka, S.2    Inouye, M.3    Yamasaki, M.4
  • 10
    • 0024273064 scopus 로고
    • Mutant subtilisin E with enhanced protease activity obtained by site-directed mutagenesis
    • Takagi, H., Morinaga, Y., Ikemura, H. & Inouye, M. 1988 Mutant subtilisin E with enhanced protease activity obtained by site-directed mutagenesis. Journal of Biological Chemistry 263, 19592-19596.
    • (1988) Journal of Biological Chemistry , vol.263 , pp. 19592-19596
    • Takagi, H.1    Morinaga, Y.2    Ikemura, H.3    Inouye, M.4
  • 11
    • 0025232324 scopus 로고
    • Enhancement of the thermostability of subtilisin E by introduction of a disulfide bond engineered on the basis of structural comparison with a thermophilic serine protease
    • Takagi, H., Takahashi, T., Momose, H., Inouye, M. Maeda, Y., Matsuzawa, H. & Ohta, T. 1990 Enhancement of the thermostability of subtilisin E by introduction of a disulfide bond engineered on the basis of structural comparison with a thermophilic serine protease. Journal of Biological Chemistry 265, 6874-6878.
    • (1990) Journal of Biological Chemistry , vol.265 , pp. 6874-6878
    • Takagi, H.1    Takahashi, T.2    Momose, H.3    Inouye, M.4    Maeda, Y.5    Matsuzawa, H.6    Ohta, T.7
  • 12
    • 0032442236 scopus 로고    scopus 로고
    • Random mutagenesis into the conserved Gly154 subtilisin E: Isolation and characterization of the revertant enzymes
    • Takagi, H., Yamamoto, M., Ohtsu, I. & Nakamori, S. 1998 Random mutagenesis into the conserved Gly154 subtilisin E: isolation and characterization of the revertant enzymes. Protein Engineering 11, 1205-1210.
    • (1998) Protein Engineering , vol.11 , pp. 1205-1210
    • Takagi, H.1    Yamamoto, M.2    Ohtsu, I.3    Nakamori, S.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.