Overexpression of the soluble form of chicken cystatin in Escherichia coli and its purification

Journal of Agricultural and Food Chemistry

Volumn 48, Issue 6, 2000, Pages 2602-2607

  Chen, Gen Hung ^a   Tang, Shye Jye ^a   Chen, Ching San ^b   Jiang, Shann Tzong ^a  

^a NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

^b INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

Author keywords

Chicken cystatin; Cysteine proteinase inhibitor; E. coli AD494(DE3)pLysS; Overexpression; PET 23a(+)

Indexed keywords

CATHEPSIN; COMPLEMENTARY DNA; CYSTATIN; CYSTEINE PROTEINASE INHIBITOR; MESSENGER RNA; PAPAIN; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; CHICKEN; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; LUNG; METABOLISM; METHODOLOGY; MOLECULAR CLONING; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; THERMOSTABILITY;

ANIMALS; CATHEPSINS; CHICKENS; CLONING, MOLECULAR; CYSTATINS; CYSTEINE PROTEINASE INHIBITORS; DNA, COMPLEMENTARY; ESCHERICHIA COLI; KINETICS; LUNG; PAPAIN; RECOMBINANT PROTEINS; RNA, MESSENGER;

EID: 0033937731     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf000058x     Document Type: Article

References (57)

1. Abe, K.; Hiroto, K.; Arai, S. Purification and characterization of a rice cysteine proteinase inhibitor. Agric. Biol. Chem. 1987, 51, 2763-2765.
2. 2-terminal 21 amino acid residues are not essential for the papain-inhibitory activity of oryzacystatin, a member of cystatin superfamily. J. Biol. Chem. 1988, 236, 7655-7659.
3. Abe, M.; Abe, K.; Kuroda, M.; Arai, S. Corn kernel cysteine proteinase inhibitor as a novel cystatin superfamily member of plant origin. Molecular cloning and expression studies. Eur. J. Biochem. 1992, 209, 933-937.
4. Abrahamson, M.; Ritonja, A.; Brown, M. A.; Grubb, A.; Machleidt, W.; Barrett, A. J. Identification of the probable inhibitory reactive sites of the cysteine proteinase inhibitors human cystatin c and chicken cystatin. J. Biol. Chem. 1987, 262, 9688-9694.
5. Abrahamson, M.; Mason, R. W.; Hansson, H.; Buttle, D. J.; Grubb, A.; Ohlsson, K. Human cystatin C. role of the N-terminal segment in the inhibition of human cysteine proteinases and in its inactivation by leucocyte elastase. Biochem. J. 1991, 273, 621-626.
6. An, H.; Seymour, T. A.; Wu, J. W.; Morrissey, M. T. Assay systems and characterization of Pacific whiting (Merluccius productus) protease. J. Food Sci. 1994, 59, 277-281.
7. Anastasi, A.; Brown, M. A.; Kembhavi, A. A.; Nicklin, M. J. H.; Sayers, C. A.; Sunter, D.; Barrett, A. J. Cystatin, a protein inhibitor of cysteine proteinases. Improved purification from egg white, characterization, and detection in chicken serum. Biochem. J. 1983, 211, 129-138.
8. Auerswald, E. A.; Genenger, G.; Assfalg-Machleidt, I.; Machleidt, W.; Engh, R. A.; Fritz, H. Recombinant chicken egg white cystatin variants of the QLVSG region. Eur. J. Biochem. 1992, 209, 837-845.
9. Auerswald, E. A.; Nagler, D. K.; Assfalg-Machleidt, I.; Stubbs, M. T.; Werner, M.; Hans, F. Hairpin loop mutations of chicken cystatin have different effects on the inhibition of cathepsin B, cathepsin L and papain. FEBS Lett. 1995, 361, 179-184.
10. Barrett, A. J. The cystatins: a new class of peptidase inhibitors. Trends Biochem. Sci. 1987, 12, 193-196.
11. Barrett, A.; Rawlings, N.; Davies, M.; Machleidt, W.; Salvesen, G.; Turk, V. In Proteinase Inhibitors; Elsevier Science Publishers: Amsterdam, The Netherlands, 1986; pp515-569.
12. Bedi, G. S.; Zhou, T.; Bedi, S. K. Production of rat salivary cystatin S variant polypeptides in Escherichia coli. Arch. Oral. Biol. 1998, 43, 173-182.
13. Bjork, I.; Ylinenjarivi, K. Interaction between chicken cystatin and the cysteine proteinases actinidin, chymopapain a, and ficin. Biochemistry 1990, 29, 1770-1776.
14. Bjork, I.; Ylinenjarvi, K. Different roles of the two disulfide bonds of the cysteine proteinase inhibitor, chicken cystatin, for the conformation of the active protein. Biochemistry 1992, 31, 8597-9602.
15. Bjork, I.; Alriksson, E.; Ylinenjarivi, K. Kinetics of binding of chicken cystatin to papain. Biochemistry 1989, 28, 1568-1573.
16. Bjork, I.; Pol, E.; Raub-Segall, E.; Abrahamson, M.; Rowan, A. D.; Mort, J. S. Differential changes in the association and dissociation rate constants for binding of cystatins to target proteinases occurring on N-terminal truncation of the inhibitors indicate that the interaction mechanism varies with different enzymes. Biochem. J. 1994, 299, 219-225.
17. Bjork, I.; Brieditis, I.; Abrahamson, M. Probing the functional role of the N-terminal region of cystatins by equilibrium and kinetic studies of the binding of Gly-11 variants of recombinant human cystatin C to target proteinases. Biochem. J. 1995, 306, 513-518.
18. Bobek, L. A.; Ramasubbu, N.; Wang, X.; Weaver, T. R.; Levine, M. J. Biological activities and secondary structures of variant forms of human salivary cystatin SN produced in Escherichia coli. Gene 1994, 151, 303-308.
19. Bode, W.; Engh, R. A.; Musil, D.; Thiele, U.; Huber, R.; Karshikov, A.; Brzin, J.; Kos, J.; Turk, V. The 2.0 A X-ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases. EMBO J. 1988, 7, 2593-2599.
20. Brzin, J.; Kopitar, M.; Turk, V. Protein inhibitors of cysteine proteinases I. Isolation and characterization of stefin, a cytosolic protein inhibitor of cysteine proteinases from human polymorphnuclear granulocytes. Hoppe-Seyler Z. Biol. Chem. 1983, 364, 1475-1478.
21. Brzin, J.; Ritonja, A.; Popovic, T.; Turk, V. Low molecular mass protein inhibitor of cysteine proteinases from soybean. Hoppe-Seyler Z. Biol. Chem. 1990, 377, 167-170.
22. Colella, R.; Sakaguchi, Y.; Nagase, H.; Bird, J. W. Chicken egg white cystatin. Molecular cloning, nucleotide sequence, and tissue distribution. J. Biol. Chem. 1989, 264, 17164-17169.
23. Derman, A. I.; Prinz, W. A.; Belin, D.; Beckwith, J. Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli. Science 1993, 262, 1744-1747.
24. Fong, D.; Kartasova, T.; Sloane, B. F.; Chan, M. M. Y. Bacterial expression of human cysteine proteinase inhibitor stefin A. FEBS Lett. 1989, 257, 55-58.
25. Fossum, K.; Whitaker, J. R. Ficin and papain inhibitor from chicken egg white. Arch. Biochem. Biophys. 1968, 125, 367-375.
26. Gerhartz, B.; Ekiel, I.; Abrahamson, M. Two stable unfolding intermediates of the disease-causing L68Q variant of human cystatin C. Biochemistry 1998, 37, 17309-17317.
27. Grubb, A.; Lofberg, H.; Barrett, A. J. The disulphide bridge of human cystatin C (y-trace) and chicken cystatin. FEBS Lett. 1984, 270, 370-374.
28. Hanahan, D.; Meselson, M. Plasmid screening at high colony density. Gene 1980, 10, 63-67.
29. Jerala, R.; Trstenjak, M.; Lenarcic, B.; Turk, V. Cloning a synthetic gene for human stefin B and its expression in E. coli. FEBS Lett. 1988, 239, 41-44.
30. Jiang, S. T.; Chen, G. H. Effect of Cathepsins B, L, L-like and Calpain on the Protein Degradation of Surimi. In Quality Attributes of Muscle Foods; Xiong, Y. L., Ho, C. T., Shahidi, F., Eds.; ACS Symposium Series 215; Kluwer Academic/Plenum Publishers: New York, 1998; Chapter 26, pp 393-405.
31. Jiang, S. T.; Lee, J. J.; Chen, H. C. Purification and characterization of cathepsin B from ordinary muscle of mackerel (Scomber australasicus). J. Agric. Food Chem. 1994, 42, 1073-1079.
32. Jiang, S. T.; Lee, B. L.; Tsao, C. Y.; Lee, J. J. Mackerel cathepsins B and L effects on thermal degradation of surimi. J. Food Sci. 1997, 62, 310-315.
33. Kaji, H.; Kumagai, I.; Takeda, A.; Miura, K. I.; Samejima, T. Studies on chemical synthesis of human cystatin A gene and its expression in E. coli. J. Biochem. 1989, 105, 143-147.
34. Kaji, H.; Samejima, T.; Kumagai, I.; Hibino, T.; Miura, K. I.; Takeda, A. Efficient preparation of human recombinant cystatin A by Escherichia coli. Hoppe-Seyler Z. Biol. Chem. 1990, 371, 145-150.
35. Katunuma, N.; Yamato, M.; Kominami, E.; Ike, Y. Total synthesis of the cystatin α-gene and its expression in E. coli. FEBS Lett. 1988, 238, 116-118.
36. Keilova, H.; Tomasek, V. Effect of papain inhibitor from chicken egg white on cathepsin B1. Biochim. Biophys. Acta 1974, 334, 179-186.
37. 4. Nature (London) 1970, 277, 680-685.
38. Lee, J. J.; Chen, H. C.; Jiang, S. T. Purification and characterization of proteinases identified as cathepsin L and L-like (58 kDa) from mackerel (Scomber australasicus). Biosci., Biotechnol., Biochem. 1993, 57, 1470-1476.
39. Lee, J. J.; Tzeng, S. S.; Jiang, S. T. Purification and characterization of low molecular weight kininogen from pig plasma. J. Food Sci. 2000, 65, 81-86.
40. Lindahl, P.; Alriksson, E.; Jornvall, H.; Bjork, I. Interaction of the cysteine proteinase inhibitor chicken cystatin with papain. Biochemistry 1988, 27, 5074-5082.
41. Lindahl, P.; Abrahamson, M.; Bjork, I. Interaction of recombinant human cystatin C with the cysteine proteinases papain and actinidin. Biochem. J. 1992a, 281, 49-55.
42. Lindahl, P.; Nycander, M.; Ylinenjarvi, K.; Pol, E.; Bjork, I. Characterization by rapid-kinetic and equilibrium methods of the interaction between N-terminally truncated forms of chicken cystatin and the cysteine proteinases papain and actinidin. Biochem. J. 1992b, 286, 165-171.
43. Machleidt, W.; Thiele, U.; Laber, B.; Assfalg-Machleidt, I.; Esterl, A.; Wiegand, G.; Kos, J.; Turk., V.; Bode, W. Mechanism of inhibition of papain by chicken egg white cystatin. Inhibition constants of N-terminally truncated forms and cyanogen bromide fragments of the inhibitor. FEBS Lett. 1989, 243, 234-238.
44. 10 bond. FEBS Lett. 1995, 361, 185-190.
45. Neuhoff, V.; Arold, N.; Toube, D.; Ehrhardt, W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear backgroung at nanogram sensitivity using coomassie brilliant blue G-250 and R-250. Electrophoresis 1988, 9, 255-262.
46. Nicklin, M. J. H.; Barrett, A. J. Inhibition of cysteine proteinases and dipeptidyl peptidase I by egg-white cystatin. Biochem. J. 1984, 223, 245-253.
47. Rowan, A. D.; Brzin, J.; Buttle, D. J.; Barrett, A. J. Inhibition of cysteine proteinases by a protein inhibitor from potato. FEBS Lett. 1990, 269, 328-330.
48. Sambrook, J.; Fritsch, E. F.; Maniatis, T. In Molecular Cloning, a Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989; Vol. 1.
49. Sharma, A.; O'Connell, B. C.; Tabak, L. A.; Bedi, G. S. Expression of a functional rat salivary cystatin S polypeptide in Escherichia coli. Arch. Oral. Biol. 1995, 40, 639-644.
50. Strauss, M.; Stollwerk, J.; Lenarcic, B.; Turk, V.; Jany, K. D.; Gassen, H. G. Chemical synthesis of a gene for human stefin A and its expression in E. coli. Hoppe-Seyler Z. Biol. Chem. 1988, 369, 1010-1030.
51. Stubbs, M. T.; Laber, B.; Bode, W.; Huber, R.; Jerala, R.; Lenarcic, B.; Turk, V. The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with the cysteine proteinase papain: a novel type of proteinase inhibitor interaction. EMBO J. 1990, 9, 1939-1947.
52. 1,2] stefin B. Hoppe-Seyler Z. Biol. Chem. 1988, 369, 1167-1178.
53. Turk, V.; Bode, W. The cystatins: protein inhibitors of cysteine proteinases. FEBS Lett. 1991, 285, 213-219.
54. Wasson, D. H.; Reppond, K. D.; Babbitt, J. K.; French, J. S. Effects of additives on proteolytic and functional properties of arrowtooth flounder surimi. J. Aquat. Food Prod. Technol. 1992a, 1 (3/4), 147-165.
55. Wasson, D.; Babbitt, J. K.; French, J. S. Characterization of a heat stable protease from arrowtooth flounder, Atheresthes stomias. J. Aquat. Food Prod. Technol. 1992b, 1 (3/4), 167-182.
56. Yamashita, M.; Konagaya, S. Purification and characterization of cathepsin L from the white muscle of chum salmon, Oncorhynchus keta. Comp. Biochem. Physiol. 1990, 96B, 247-252.
57. Zerovnik, E.; Cimerman, N.; Kos, J.; Turk, V.; Lohner, K. Thermal denaturation of human cystatin C and two of its variants; comparison to chicken cystatin. Biol. Chem. 1997, 378, 1199-1203.