Pseudoleukocytosis without pseudothrombocytopenia induced by the interaction of EDTA and IgG2-κ M-protein

Clinica Chimica Acta

Volumn 299, Issue 1-2, 2000, Pages 119-128

  Shimasaki, Akemi Kubo ^a   Fujita, Kiyotaka ^b   Fujio, Syouko ^a   Sakurabayashi, Ikunosuke ^c  

^a NATIONAL HOSPITAL ORGANIZATION HIMEJI MEDICAL CENTER   (Japan)

^b SHINSHU UNIVERSITY   (Japan)

^c JICHI MEDICAL UNIVERSITY   (Japan)

Author keywords

EDTA; IgG2; M protein; Pseudoleukocytosis

Indexed keywords

EDETIC ACID; IMMUNOGLOBULIN G2; M PROTEIN;

ADULT; ARTICLE; CASE REPORT; ELECTROPHORESIS; HUMAN; LABORATORY TEST; LEUKOCYTOSIS; MALE; MONOCLONAL IMMUNOGLOBULINEMIA; PRIORITY JOURNAL; SIDE EFFECT; THROMBOCYTOPENIA; WESTERN BLOTTING;

ANTIBODIES, MONOCLONAL; ANTICOAGULANTS; BLOTTING, WESTERN; BONE MARROW; EDETIC ACID; ELECTROPHORESIS, CELLULOSE ACETATE; HUMANS; IMMUNOELECTROPHORESIS; LEUKOCYTE COUNT; LEUKOCYTOSIS; MALE; MIDDLE AGED; PARAPROTEINEMIAS; TEMPERATURE; THROMBOCYTOPENIA;

EID: 0033935377     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-8981(00)00287-4     Document Type: Article

References (18)

1. Gowland E., Kay H.E.M., Spillman J.C., Williamson J.R. Agglutination of platelets by a serum factor in the presence of EDTA. J Clin Pathol. 22:1969;460-464.
2. Pegels J.G., Bruynes E.C., Engelfriet C.P., von dem Borne A.E. Pseudothrombocytopenia: an immunologic study on platelet antibodies dependent on ethylene diamine tetra-acetate. Blood. 59:1982;157-161.
3. Lombarts A.J.P.F., Kieviet W. Recognition and prevention of pseudothrombocytopenia and concomitant pseudoleukocytosis. Am J Clin Pathol. 89:1988;634-639.
4. Shimasaki A.K., Kato T., Ozaki Y. Studies of platelet aggregation in six cases of EDTA-dependent pseudothrombocytopenia. Jpn J Clin Hematol. 35:1994;529-534.
5. Edwin G.T., Grossman J., Abraham G.N., Leddy J.P., Lichtman M.A. Pseudoleukocytosis due to cryoprotein crystals. Am J Clin Pathol. 60:1973;669-671.
6. Banfi G., Bonini P.A. Detection of cryoglobulins by Coulter Counter model S-Plus IV/D. Clin Lab Haematol. 10:1988;453-459.
7. Woltjen H.H., Hansmann E., Bremer K. Pseudoleukocytosis in cryoglobulinemia type I. Dtsch Med Wochenschr. 118:1993;260-264.
8. Zandecki M., Dupriez B., Fenaux P., et al. Cytological and ultrastructural assessment of free crystals or precipitates associated with pseudoleukocytosis and pseudothrombocytosis in cryoglobulinemia. Nouv Rev Fr Hematol. 31:1989;397-402.
9. Shimasaki A.K., Ozaki Y. Platelet aggregate formation in peripheral blood anti-coagulated with sodium heparin is facilitated by mixing. Jpn J Clin Hematol. 38:1997;323-330.
10. Anderson G.P. Insights into heparin-induced thrombocytopenia. Br J Haematol. 80:1992;504-508.
11. Scheidegger J.J. Une micro-methode de l'immuno-electrophorese. Int Arch Allergy. 7:1955;103-110.
12. Towbin H., Staechelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA. 76:1979;4350-4354.
13. Roitt I., Brostoff J., Male D. Immunology. 1985;47-55 Nankogo/Gower, Tokyo.
14. Riesen W., Morell A. A human myeloma protein with specificity against dinitrophenyl and nucleic acid derivatives. Immunochemistry. 9:1972;979-985.
15. Fredman M., Merrett R., Pruzanski W. Human monoclonal immunoglobulins with antibody-like activity. Immunochemistry. 13:1976;193-202.
16. Sawada S., Shida M., Suenaga R., et al. Elicited antibody nature of human monoclonal protein with anti-streptolysin O activity. Analysis with monoclonal anti-idiotype antibody. J Clin Lab Immunol. 19:1986;31-35.
17. Fujita K., Sakurabayashi I., Yoshida N., Nishiguchi T., Teramura Y., Kawai T. Immunochemical properties of IgG-κ type M-protein bound to albumin in human serum. Phys Chem Biol. 36:1992;133-138.
18. 1-macroglobulin. Clin Chim Acta. 149:1985;205-214.