A quantitative assay for the non-covalent association between apoliprotein[a] and apolipoprotein B: An alternative measure of Lp[a] assembly

Journal of Lipid Research

Volumn 41, Issue 6, 2000, Pages 1013-1019

  Dardik, B N ^a   Schwartzkopf, C D ^a   Stevens, D E ^a   Chatelain, R E ^a  

^a NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

Lp a formation; Nicotinic acid

Indexed keywords

APOLIPOPROTEIN A; APOLIPOPROTEIN B; CAPTOPRIL; NICOTINIC ACID;

ANALYTIC METHOD; ARTICLE; COMPLEX FORMATION; COVALENT BOND; DISULFIDE BOND; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN LIPID INTERACTION; PROTEIN STABILITY; QUANTITATIVE ASSAY;

APOLIPOPROTEINS; APOLIPOPROTEINS B; APOPROTEIN(A); FLUORESCEIN-5-ISOTHIOCYANATE; HUMANS; IODINE RADIOISOTOPES; LIPOPROTEIN(A); LIPOPROTEINS, LDL; LYSINE; PROLINE; PROTEIN BINDING; PROTEIN PROCESSING, POST-TRANSLATIONAL;

EID: 0033934624     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. Berg, K., G. Dahlen, and M. H. Frick. 1974. Lp(a) lipoprotein and pre-β1-lipoprotein in patients with coronary heart disease. Clin. Genet. 6: 230-235.
2. Bostom, A. G., L. A. Cupples, J. L. Jenner, J. M. Ordovas, L. J. Seman, P. W. F. Wilson, E. J. Schaefer, and W. P. Castelli. 1996. Elevated plasma lipoprotein(a) and coronary heart disease in men aged 55 years and younger. J. Am. Med. Assoc. 276: 544-548.
3. Jurgens, G., W. C. Taddei-Peters, P. Koltringer, W. Petek, Q. Greilberger, P. F. Macomber, B. T. Butman, A. G. Stead, and J. H. Ransom. 1995. Lipoprotein(a) serum concentration and apoprotein(a) phenotype correlate with severity and presence of ischemic cerebrovascular disease. Stroke. 26: 1841-1848.
4. Utermann, G. 1989. The mysteries of lipoprotein(a). Science. 246: 904-910.
5. McLean, J. W., J. Tomlinson, J. Kuang, D. Eaton, E. Chen, G. Fless, A. Scanu, and R. M. Lawn. 1987. cDNA sequence of human apolipoprotein(a) is homologous to plasminogen. Nature. 330: 132-137.
6. Boerwinkle, E., C. C. Leffert, J. Lin, C. Lackner, G. Chiesa, and H. H. Hobbs. 1992. Apolipoprotein(a) gene accounts for greater than 90% of the variation in plasma lipoprotein(a) concentrations. J. Clin. Invest. 90: 52-60.
7. Krempler, F., G. M. Kostner, K. Bolzano, and F. Sandhofer. 1980. Turnover of lipoprotein(a) in man. J. Clin. Invest. 65: 1483-1490.
8. Rader, D. J., W. Cain, L. A. Zech, D. Usher, and H. B. Brewer, Jr. 1993. Variation in lipoprotein(a) concentrations among individuals with the same apolipoprotein(a) isoform is determined by the rate of lipoprotein(a) production. J. Clin. Invest. 91: 443-447.
9. Rader, D. J., W. Cain, K. Ikewaki, G. Talley, L. A. Zech, D. Usher, and H. B. Brewer, Jr. 1994. The inverse association of plasma lipoprotein(a) concentrations with apolipoprotein(a) isoform size is not due to differences in Lp(a) catabolism but to differences in production rate. J. Clin. Invest. 93: 2758-2763.
10. Gurakar, A., J. M. Hoeg, G. Kostner, N. M. Papadopoulos, and H. B. Brewer, Jr. 1985. Levels of lipoprotein Lp(a) decline with neomycin and niacin treatment. Atherosclerosis. 57: 293-301.
11. Alberts, J. J., H. M. Taggart, D. Applebaum-Bowden, S. Haffner, C. H. Chestnut III, and W. R. Hazzard. 1984. Reduction of lecithin-cholesterol acyltransferase, apolipoprotein D and the Lp(a) lipoprotein with the anabolic steroid stanazolol. Biochim. Biophys. Acta. 795: 293-296.
12. Farish, E., H. A. Rolton, J. F. Barnes, and D. M. Hart. 1991. Lipoprotein(a) concentrations in postmenopausal women taking norethisterone. Br. Med. J. 303: 694.
13. White, A. L., D. L. Rainwater, and R. E. Lanford. 1993. Intracellular maturation of apolipoprotein[a] and assembly of lipoprotein[a] in primary baboon hepatocytes. J. Lipid Res. 34: 509-517.
14. White, A. L., and R. E. Lanford. 1994. Cell surface assembly of lipoprotein[a] in primary cultures of baboon hepatocytes. J. Biol. Chem. 269: 28716-28723.
15. Koschinsky, M. L., J. E. Tomlinson, T. F. Zioncheck, K. Schwartz, D. L. Eaton, and R. M. Lawn. 1991. Apolipoprotein(a): expression and characterization of a recombinant form of the protein in mammalian cells. Biochemistry. 30: 5044-5051.
16. Koschinsky, M. L., G. P. Cote, B. Gabel, and Y. Y. van der Hoek. 1993. Identification of the cysteine residue in apolipoprotein[a] that mediates extracellular coupling with apolipoprotein B-100. J. Biol. Chem. 268: 19819-19825.
17. Edelstein, C., N. O. Davidson, and A. M. Scanu. 1994. Oleate stimulates the formation of triglyceride-rich particles containing apoB100-apo(a) in long-term primary cultures of human hepatocytes. Chem. Phys. Lipids. 67/68: 135-143.
18. Bonen, D. K., A. M. L. Hausman, C. Hadjiagapiou, S. F. Skarosi, and N. O. Davidson. 1997. Expression of a recombinant apolipoprotein(a) in HepG2 cells. Evidence for intracellular assembly of lipoprotein(a). J. Biol. Chem. 272: 5659-5667.
19. Phillips, M. L., A. V. Lembertas, B. N. Schumaker, R. M. Lawn, S. J. Shire, and T. F. Zioncheck. 1993. Physical properties of recombinant apolipoprotein(a) and its association with LDL to form an Lp(a)-like complex. Biochemistry. 32: 3722-3728.
20. 4057 of apolipoprotein(a) is essential for lipoprotein(a) assembiy. Proc. Natl. Acad. Sci. USA. 90: 11643-11647.
21. Frank, S., K. Krasznai, S. Durovic, E. M. Lobentanz, H. Dieplinger, E. Wagner, K. Zatloukal, M. Cotten, G. Utermann, G. M. Kostner, and R. Zechner. 1994. High-level expression of various apolipoprotein(a) isoforms by "transferrinfection": the role of kringle IV sequences in the extracellular association with low-density lipoprotein. Biochemistry. 33: 12329-12339.
22. Trieu, V. N., and W. J. McConathy. 1995. A two-step model for lipoprotein(a) formation. J. Biol. Chem. 270: 15471-15474.
23. Koschinsky, M. L., S. M. Marcovina, L. F. May, and B. R. Gabel. 1997. Analysis of the mechanism of lipoprotein(a) assembly. Clin. Genet. 52: 338-346.
24. 2 and other apolipoprotein B-comaining lipoproteins with lipoprotein(a). J. Biol. Chem. 263: 6337-6343.
25. Ernst, A., M. Helmhold, C. Brunner, A. Petho-Schramm, V. W. Armstrong, and H. J. Muller. 1995. Identification of two functionally distinct lysine-binding sites in kringle 37 and in kringles 32-36 of human apolipoprotein (a). J. Biol. Chem. 270: 6227-6234.
26. Trieu, V. N., and W. J. McConathy. 1990. Lipoprotein(a) binding to other apolipoprotein B-containing lipoproteins. Biochemistry. 29: 5919-5924.
27. Havel, R. J., H. A. Eder, and J. H. Bragdon. 1955. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J. Clin. Invest. 34: 1345-1353.
28. Fless, G. M., C. A Rolih, and A. M. Scanu. 1984. Heterogeneity of human plasma lipoprotein(a). Isolation and characterization of the lipoprotein subspecies and their apoproteins. J. Biol. Chem. 259: 11470-11478.
29. Eaton, D. L., G. M. Fless, W. J. Kohr, J. W. McLean, Q. T. Xu, C. G. Miller, R. M. Lawn, and A. M. Scanu. 1987. Partial amino acid sequence of apolipoprotein(a) shows that it is homologous to plasminogen. Proc. Natl. Acad. Sci. USA. 84: 3224-3228.
30. Edelstein, C. M., M. Mandala, D. Pfaffinger, and A. M. Scanu. 1995. Determinants of lipoprotein(a) assembly: a study of wild-type and mutant apolipoprotein(a) phenotypes isolated from human and rhesus monkey lipoprotein(a) under mild reductive conditions. Biochemistry. 34: 16483-16492.
31. Brinkley, M. 1992. A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents. Bioconjugate Chem. 3: 2-13.
32. Trieu, V. N., T. F. Zioncheck, R. M. Lawn, and W. J. McConathy. 1991. Interaction of apolipoprotein(a) with apolipoprotein B-containing lipoproteins. J. Biol. Chem. 266: 5480-5485.
33. Frank, S., S. Durovic, K. Kostner, and G. M. Kostner. 1995. Inhibitors for the in vitro assembly of Lp(a). Arterioscler. Thromb. Vasc. Biol. 15: 1774-1780.
34. Medical Economics. 1999. Physicians' Desk Reference. Montvale, NJ.
35. 4] captopril as internal standard. J. Chromatogr. 529: 299-308.