Characterization of prosystemin expressed in the baculovirus/insect cell system reveals biological activity of the systemin precursor

Planta

Volumn 211, Issue 1, 2000, Pages 91-97

  Vetsch, Michael ^a   Janzik, Ingar ^a   Schaller, Andreas ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Baculovirus insect cell expression; Lycopersicon (wound response); Plant defense; Prosystemin; Systemin; Wound response

Indexed keywords

ALKALINIZATION; AMINO TERMINAL SEQUENCE; INSECT CELL CULTURE; LYCOPERSICON ESCULENTUM; LYCOPERSICON PERUVIANUM; PROSYSTEMIN; PROTEIN EXPRESSION; REACTION MECHANISM; SIGNAL PEPTIDE; SYSTEMIN;

INSECTA; LYCOPERSICON; LYCOPERSICON ESCULENTUM; LYCOPERSICON PERUVIANUM; UNIDENTIFIED BACULOVIRUS;

EID: 0033934336     PISSN: 00320935     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004250000264     Document Type: Article

References (24)

1. Bergey DR, Howe GA, Ryan CA (1996) Polypeptide signaling for plant defensive genes exhibits analogies to defense signaling in animals. Proc Natl Acad Sci USA 93: 12053-12058
2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254
3. Constabel PC, Yip L, Ryan CA (1998) Prosystemin from potato, black nightshade, and bell pepper: primary structure and biological activity of predicted systemin polypeptides. Plant Mol Biol 36: 55-62
4. Farmer EE, Ryan CA (1992) Octadecanoid precursors of jasmonic acid activate the synthesis of wound-inducible proteinase inhibitors. Plant Cell 4: 129-134
5. Felix G, Boller T (1995) Systemin induces rapid ion fluxes and ethylene biosynthesis in Lycopersicon peruvianum cells. Plant J 7: 381-389
6. Harlow E, Lane D (1988) Antibodies: a laboratory manual. Cold Spring Harbor, New York
7. Jacinto T, McGurl B, Franceschi V, Delano-Freier J, Ryan CA (1997) Tomato prosystemin promoter confers wound-inducible, vascular bundle-specific expression of the β-glucuronidase gene in transgenic tomato plants. Planta 203: 406-412
8. Jay GD, Culp DJ, Jahnke MR (1990) Silver staining of extensively glycosylated proteins on sodium dodecyl sulfate-polyacrylamide gels: enhancement by carbohydrate-binding dyes. Anal Biochem 185: 324-330
9. King LA, Possee RD (1992) The baculovirus expression system: a laboratory guide. Chapman and Hall, New York
10. Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680-685
11. McGurl B, Ryan CA (1992) The organization of the prosystemin gene. Plant Mol Biol 20: 405-409
12. McGurl B, Pearce G, Orozco-Cardenas M, Ryan CA (1992) Structure, expression and antisense inhibition of the systemin precursor gene. Science 255: 1570-1573
13. Meindl T, Boller T, Felix G (1998) The plant wound hormone systemin binds with the N-terminal part to its receptor but needs the C-terminal part to activate it. Plant Cell 10: 1561-1570
14. Narváez-Vásquez J, Florin-Christensen J, Ryan CA (1999) Positional specificity of a phospholipase A activity induced by wounding, systemin, and oligosaccharide elicitors in tomato leaves. Plant Cell 11: 2249-2260
15. Pearce G, Strydom D, Johnson S, Ryan CA (1991) A polypeptide from tomato leaves induces wound-inducible proteinase inhibitor proteins. Science 253: 895-898
16. Pearce G, Johnson S, Ryan CA (1993) Structure-activity of deleted and substituted systemin, an 18-amino acid polypeptide inducer of plant defensive genes. J Biol Chem 268: 212-216
17. Realini C, Rogers SW, Rechsteiner M (1994) Proposed roles in protein-protein association and presentation of peptides by the MHC class I receptors. FEBS Lett 348: 109-113
18. Ryan CA, Pearce G (1998) Systemin: a polypeptide signal for plant defensive genes. Annu Rev Cell Dev Biol 14: 1-17
19. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
20. Schaller A (1998) Action of proteolysis-resistant systemin analogues in wound signalling. Phytochemistry 47: 605-612
21. Schaller A (1999) Oligopeptide signalling and the action of systemin. Plant Mol Biol 40: 763-769
22. +-ATPase activity differentially activates wound and pathogen defense responses in tomato plants. Plant Cell 11: 263-272
23. Schaller A, Bergey DR, Ryan CA (1995) Induction of wound response genes in tomato leaves by bestatin, an inhibitor of aminopeptidases. Plant Cell 7: 1893-1898
24. Scheer JM, Ryan CA (1999) A 160 kDa systemin receptor on the cell surface of Lycopersicon peruvianum suspension cultured cells: kinetic analyses, induction by methyl jasmonate and photoaffinity labeling. Plant Cell 11: 1525-1535