Ribonuclease from cobra snake venom: Purification by affinity chromatography and further characterization

IUBMB Life

Volumn 49, Issue 4, 2000, Pages 309-316

  Mahalakshmi, Y V ^a   Jagannadham, M V ^a   Pandit, M W ^a  

^a CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

Cobra venom; Phospholipase; Ribonuclease

Indexed keywords

RIBONUCLEASE; SNAKE VENOM;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; ENZYME ANALYSIS; ENZYME PURIFICATION; NONHUMAN; SNAKE;

SERPENTES;

EID: 0033932855     PISSN: 15216543     EISSN: None     Source Type: Journal    
DOI: 10.1080/15216540050033186     Document Type: Article

References (21)

1. Vasilenko, S. K., and Babkina, G. T. (1965) Isolation and properties of the ribonuclease from cobra venom. Biokhimiya 30, 705-712.
2. Lockard, R. E., and Kumar, A. (1981) Mapping tRNA structure in solution using double-strand-specific ribonuclease VI from cobra venom. Nucleic Acids Res. 9, 5125-5140.
3. Boguski, M. S., Hieter, P. A., and Levy, C. C. (1980) Identification of a cytidine-specific ribonuclease from chicken liver. J. Biol. Chem. 255, 2160-2163.
4. Levy, C. C., and Karpetsky, T. P. (1980) An enzyme which is useful in distinguishing between cytidylic acid and uridylic acid. J. Biol. Chem. 255, 2153-2159.
5. Hayano, K., Iwama, M., Sakamoto, H., Sanda, A., Ohgi, K., and Irie, M. (1993) Characterization of Poly C preferential ribonuclease from chicken liver. J. Biochem. (Tokyo) 114, 156-162.
6. Mahalakshmi, Y. V., and Pandit, M. W. (1987) A new ribonuclease from cobra venom (Naja naja) showing specificity towards cytidylic acid. Biochem. Biophys. Res. Commun. 145, 740-748.
7. Heide, K., and Shwick, H.C. (1978) Salt fractionation of immunoglobulins: Isolation of pure IgG using sodium sulphate. Immunochemistry, In Hand Book of Experimental Immunology, Vol. 1 (Weir, D. M., ed.). pp. 7.1-7.11, Blackwell Scientific Publications, Oxford.
8. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
9. Wray, W., Boulikas, T., Wray, V. P., and Hancock, R. (1981) Silver staining of proteins in polyacrylamide gels. Anal. Biochem. 118, 197-203.
10. Davis, B. J. (1964) Disc electrophoresis - II. Methods and application to human serum proteins. Ann. N. Y. Acad. Sci. 121, 404-427.
11. Kalnitsky, G., Hummel, J. P., Resnick, H., Carter, J. R., Barnett, L. B., and Dierks, C. (1959) The relation of structure to enzymatic activity in ribonuclease. Ann. N. Y. Acad. Sci. 81, 542-569.
12. MacFarlane, M. G., and Knight, B. C. J. G. (1941) The biochemistry of bacterial toxins. I. The lecithinase activity of Cl. welchii toxins. Biochem. J. 35, 884-902.
13. Koerner, J. F., and Sinsheimer, R. L. (1957) A deoxyribonuclease from calf spleen. II. Mode of action. J. Biol. Chem. 228, 1049-1062.
14. Silman, I. H., Albu-Weissenberg, M., and Katchalski, E. (1966) Some water-soluble papain derivatives. Biopolymers 4, 441-448.
15. Sierakowska, H., and Shugar, D. (1977) Mammalian Nucleolytic Enzymes. Prog. Nucleic Acids Res. Mol. Biol. 20, 59-130.
16. Babkina, G. T., and Vasilenko, S. I. (1964) Nuclease activity of venoms of Central Asian snakes. Biokhimiya 29, 268-272.
17. Amara Sagar, J., Mahalakshmi, Y. V. Nagaraj, R., and Pandit, M. W. (1989) Synthetic peptides corresponding to the sites 37-42 and 83-88 on ribonuclease A show immunogenicity. Int. J. Peptide Protein Res. 33, 452-456.
18. Smyth, D. G., Stein, W. H., and Moore, S. (1963) The sequence of amino acid residues in bovine pancreatic ribonuclease: Revisions and confirmations. J. Biol. Chem. 238, 227-234.
19. Zhao, W., Beintema, J. J., and Hofsteenge, J. (1994) The amino acid sequence of iguana (Iguana iguana) pancreatic ribonuclease. Eur. J. Biochem. 219, 641-646.
20. Barnard, E. A., Cohen, M. S., Gold, M. H. and Kim, J. K. (1972) Evolution of ribonuclease in relation to polypeptide folding mechanisms. Nature 240, 395-398.
21. Altsehul, S. F., Madden,T. L., Sehaffer, A. A., Jinghui, Z., Zhang, Z., Miller, W., and Lipman, J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402.