Identification and biochemical localization of a Na-K-Cl cotransporter in the human placental cell line BeWo

Biochemical and Biophysical Research Communications

Volumn 274, Issue 1, 2000, Pages 43-48

  Zhao, Haiyan ^a   Hundal, Harinder S ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

Bumetanide; Cell volume; Membrane; Na pump; Na,K ATPase; Placenta; Transport; Triple cotransporter; Trophoblast

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); BUMETANIDE; CARRIER PROTEIN; CHLORIDE ION; MEMBRANE PROTEIN; POTASSIUM ION; RUBIDIUM 86; RUBIDIUM ION; SODIUM ION;

ARTICLE; CELL LINE; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; ION TRANSPORT; OSMOTIC STRESS; PLACENTA; PRIORITY JOURNAL; PROTEIN LOCALIZATION; TROPHOBLAST;

EID: 0033932634     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.3099     Document Type: Article

References (24)

1. Stulc J. Placental transfer of inorganic ions and water. Physiol. Rev. 77:1997;805-836.
2. Shennan D. B. Recent advances in placental ion transport. Comp. Biochem. Physiol. 101:1992;187-193.
3. Mahendran D., Donnai P., Glazier J. D., D'Souza S. W., Boyd R. D., Sibley C. P. Amino acid (system A) transporter activity in microvillous membrane vesicles from the placentas of appropriate and small for gestational age babies. Pediat. Res. 34:1993;661-665.
4. Illsley N. P., Glaubensklee C., Davis B., Verkman A. S. Chloride transport across placental microvillous membranes measured by fluorescence. Am. J. Physiol. 255:1988;C789-C797.
5. Dechecchi M. C., Cabrini G. Chloride conductance in membrane vesicles from human placenta using a fluorescent probe. Implications for cystic fibrosis. Biochim. Biophys. Acta. 945:1988;113-120.
6. Byrne S., Glazier J. D., Greenwood S. L., Mahendran D., Sibley C. P. Chloride transport by human placental microvillous membrane vesicles. Biochim. Biophys. Acta. 1153:1993;122-126.
7. Powell T. L., Lundquist C., Doughty I. M., Glazier J. D., Jansson T. Mechanisms of chloride transport across the syncytiotrophoblast basal membrane in the human placenta. Placenta. 19:1998;315-321.
8. Friedman S. J., Skehan P. Morphological differentiation of human choriocarcinoma cells induced by methotrexate. Cancer Res. 39:1979;1960-1967.
9. Furesz T. C., Smith C. H., Moe A. J. ASC system activity is altered by development of cell polarity in trophoblast from human placenta. Am. J. Physiol. 265:1993;C212-C217.
10. Moe A. J., Furesz T. C., Smith C. H. Functional-characterization of L-alanine transport in a placental choriocarcinoma cell-line (BeWo). Placenta. 15:1994;797-802.
11. Qian Y. C., Majumdar S., Reddy M. C. M., Harris E. D. Coincident expression of menkes gene with copper efflux in human placental cells. Am. J. Physiol. 270:1996;C1880-C1884.
12. Qian Y. C., Tiffanycastiglioni E., Harris E. D. Functional-analysis of a genetic-defect of copper transport (menkes disease) in different cell-lines. Am. J. Physiol. 271:1996;C378-C384.
13. Van der Ende A., Dumaine A., Schwartz A. L., Strous G. J. Effect of ATP depletion and temperature on the transferrin-mediated uptake and release of iron by BeWo choriocarcinoma cells. Biochem. J. 259:1989;685-692.
14. Shah S. W., Zhao H., Low S. Y., McArdle H. J., Hundal H. S. Characterization of glucose transport and glucose transporters in the human choriocarcinoma cell line, BeWo. Placenta. 20:1999;651-659.
15. Hajduch E., Alessi D. R., Hemmings B. A., Hundal H. S. Constitutive activation of Protein Kinase Bα (PKBα) by membrane targeting promotes glucose and System A amino acid transport, protein synthesis and GSK3 inactivation in L6 muscle cells. Diabetes. 47:1998;1006-1013.
16. Hundal H. S., Ramlal T., Reyes R., Leiter L. A., Klip A. Cellular mechanism of metformin action involves glucose transporter translocation from an intracellular pool to the plasma membrane in L6 muscle cells. Endocrinology. 131:1992;1165-1173.
17. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
18. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
19. Hundal H. S., Maxwell D. L., Ahmed A., Darakhshan F., Mitsumoto Y., Klip A. Subcellular distribution and immunocytochemical localization of Na,K-ATPase subunit isoforms in human skeletal muscle. Mol. Membr. Biol. 11:1994;255-262.
20. Clarson L. H., Glazier J. D., Greenwood S. L., Jones C. J., Sides M. K., Sibley C. P. Activity and expression of Na-K-ATPase in human placental cytotrophoblast cells in culture. J. Physiol. 497:1996;735-743.
21. Murphy C., Shennan D. B. J. Physiol. 386:1987;110P.
22. Liu F., Soares M. J., Audus K. L. Permeability properties of monolayers of the human trophoblast cell line BeWo. Am. J. Physiol. 273:1997;C1596-C1604.
23. Haas M. The Na-K-Cl cotransporters. Am. J. Physiol. 267:1994;C869-C885.
24. O'Grady S. M., Palfrey H. C., Field M. Characteristics and functions of Na-K-Cl cotransport in epithelial tissues. Am. J. Physiol. 253:1987;C177-C192.