Role of tryptophan-1 in hemolytic and phospholipase C activities of Clostridium perfringens alpha-toxin

Microbiology and Immunology

Volumn 44, Issue 7, 2000, Pages 585-589

  Nagahama, Masahiro ^a   Mukai, Masakazu ^a   Ochi, Sadayuki ^a   Sakurai, Jun ^a  

^a TOKUSHIMA BUNRI UNIVERSITY   (Japan)

Author keywords

Alpha toxin; Clostridium perfringens; Phospholipase C; Tryptophan residue

Indexed keywords

ALPHA TOXIN; GLYCINE; HISTIDINE; PHOSPHOLIPASE C; TRYPTOPHAN; TYROSINE; ZINC;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; CLOSTRIDIUM PERFRINGENS; ENZYME ACTIVITY; ENZYME BINDING; HEMOLYSIS; HUMAN; HUMAN CELL;

CLOSTRIDIUM PERFRINGENS; NEGIBACTERIA;

EID: 0033929841     PISSN: 03855600     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2000.tb02537.x     Document Type: Article

References (18)

1. Guillouard, I., Garnier, T., and Cole, S.T. 1996. Use of site-directed mutagenesis to probe structure-function relationships of alpha-toxin from Clostridium perfringens. Infect. Immun. 64: 2440-2444.
2. Hough, E., Hansen, L.K., Birknes, B., Jynge, K., Hansen, S., Hordvik, A., Little, C., Dodson, E., and Derewenda, Z. 1989. High-resolution (1.5 Å) crystal structure of phospholipase C from Bacillus cereus. Nature 338: 357-360.
3. Hunter, S.E.C., Brown, J.E., Oyston, P.C.F., Sakurai, J., and Titball, R.W. 1993. Molecular genetic analysis of beta-toxin of Clostridium perfringens reveals sequence homology with alpha-toxin, gamma-toxin, and leukocidin of Staphylococcus aureus. Infect. Immun. 61: 3958-3965.
4. Jepson, M., Howells, A., Bullifent, H.L., Bolgiano, B., Crane, D., Miller, J., Holley, J., Jayasekera, P., and Titball, R.W. 1999. Differences in the carboxy-terminal (Putative phospholipid binding) domains of Clostridium perfringens and Clostridium bifermentans phospholipases C influence the hemolytic and lethal properties of these enzymes. Infect. Immun. 67: 3297-3301.
5. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
6. Nagahama, M., Iida, H., Nishioka, E., Okamoto, K., and Sakurai, J. 1994. Roles of the carboxy-terminal region of Clostridium perfringens alpha toxin. FEMS Microbiol. Lett. 120: 297-302.
7. Nagahama, M., Okagawa, Y., Nakayama, T., Nishioka, E., and Sakurai, J. 1995. Site-directed mutagenesis of histidine residues in Clostridium perfringens alpha-toxin. J. Bacteriol. 177: 1179-1185.
8. Nagahama, M., Nakayama, T., Michiue, K., and Sakurai, J. 1997. Site-specific mutagenesis of Clostridium perfringens alpha-toxin: replacement of Asp-56, Asp-130, or Glu-152 causes loss of enzymatic and hemolytic activities. Infect. Immun. 65: 3489-3492.
9. Nagahama, M., Michiue, K., Mukai, M., Ochi, S., and Sakurai, J. 1998. Mechanism of membrane damage by Clostridium perfringens alpha-toxin. Microbiol. Immunol. 42: 533-538.
10. Nagahama, M., Kihara, A., Miyawaki, T., Mukai, M., Sakaguchi, Y., Ochi, S., and Sakurai, J. 1999. Clostridium perfringens beta-toxin is sensitive to thiol-group modification but does not require a thiol group for lethal activity. Biochim. Biophys. Acta 1454: 97-105.
11. Naylor, C.E., Eaton, J.T., Howells, A., Justin, N., Moss, D.S., Titball, R.W., and Basak, A.K. 1998. Structure of the key toxin in gas gangrene. Nat. Struct. Biol. 5: 738-746.
12. Sakurai, J. 1995. Toxins of Clostridium perfringens. Rev. Med. Microbiol. 6: 175-185.
13. Sakurai, J., Nagahama, M., and Ochi, S. 1997. Major toxins of Clostridium perfringens. J. Toxicol. Toxin Rev. 16: 195-214.
14. Titball, R.W., Leslie, D.L., Harvey, S., and Kelly, D. 1991. Hemolytic and sphingomyelinase activities of Clostridium perfringens alpha-toxin are dependent on a domain homologous to that of an enzyme from the human arachidonic acid pathway. Infect. Immun. 59: 1872-1874.
15. Titball, R.W. 1993. Bacterial phospholipases C. Microbiol. Rev. 57: 347-366.
16. Titball, R.W. 1998. Bacterial phospholipases. J. Appl. Microbiol. Symp. Suppl. 27: 127S-137S.
17. Titball, R.W., Naylor, C.E., and Basak, A.K. 1999. The Clostridium perfringens alpha-toxin. Anaerobe 5: 51-64.
18. Vallee, B.L., and Auld, D.S. 1993. New perspective on zinc biochemistry: cocatalytic sites in multi-zinc enzymes. Biochemistry 32: 6493-6500.