The cell surface glycoprotein layer of the extreme halophile Halobacterium salinarum and its relation to Haloferax volcanii: Cryo-electron tomography of freeze-substituted cells and projection studies of negatively stained envelopes

Journal of Structural Biology

Volumn 130, Issue 1, 2000, Pages 10-26

  Trachtenberg, Shlomo ^a   Pinnick, Baruch ^a   Kessel, Martin ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE PROTEIN; LIQUID NITROGEN; MAGNESIUM; PLASTIC; SODIUM;

ARTICLE; BACTERIAL MEMBRANE; CELL DENSITY; CELL STRUCTURE; CRYOELECTRON MICROSCOPY; CRYSTALLOGRAPHY; CULTURE MEDIUM; EMBEDDING; FREEZE DRYING; HALOBACTERIUM SALINARUM; HALOFERAX VOLCANII; HALOPHILIC BACTERIUM; NONHUMAN; PRIORITY JOURNAL; STAINING;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); HALOBACTERIUM SALINARUM; HALOFERAX VOLCANII; HALOPHILIC ARCHAEON; UNCULTURED ACTINOMYCETE;

EID: 0033927978     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsbi.2000.4215     Document Type: Article

References (24)

1. Aebi U. Misell D. L. Image Analysis Enhancement and Interpretation. 1978;112-114 North-Holland, Amsterdam.
2. Baumeister W., Lembcke G. Structural features of archaebacterial cell envelopes. J. Bioenerg. Biomembr. 24:1992;567-575.
3. Blaurock A., Stoeckenius W., Oesterhelt D., Scherphof G. Structure of the cell envelope of Halobacterium halobium. J. Cell Biol. 71:1976;1-22.
4. Bogomolni R. A., Baker R. A., Lozier R. H., Stoeckenius W. Light-driven proton translocations in Halobacterium halobium. Biochim. Biophys. Acta. 440:1976;68-88.
5. Braunfeld M. B., Koster A. J., Sedat J. W., Agard D. A. Cryo automated electron reconstruction of plastic-embedded structures. J. Microsc. 174:1994;75-84.
6. Cantino M. E., Wilkinson L. E., Goddard M. K., Johnson D. E. Beam induced mass loss in high resolution biological microanalysis. J. Microsc. 144:1986;317-327.
7. Chalcroft J. P., Davies C. L. A simply constructed extreme tilt holder for the Philips eucentric goniometer stage. J. Microsc. 134:1984;41-48.
8. Cohen S., Shilo M., Kessel M. Nature of the salt dependence of the envelope of a Dead Sea archaebacterium Haloferax volcanii. Arch. Microbiol. 156:1991;198-203.
9. Dierksen K., Typke D., Hegerl R., Koster A. J., Baumeister W. Towards automatic tomography. Ultramicroscopy. 40:1992;71-87.
10. Dierksen K., Typke D., Hegerl R., Koster A. J., Baumeister W. Towards automated tomography. II. Implementation of autofocus and low-dose procedures. Ultramicroscopy. 49:1993;109-120.
11. Grimm R., Singh H., Rachel R., Typke D., Zillig W., Baumeister W. Electron tomography of ice-embedded prokaryotic cells. Biophys. J. 74:1998;1031-1042.
12. Hegerl R. The EM program package: A platform for image processing in biological electron microscopy. J. Struct. Biol. 116:1996;30-34.
13. Kessel M., Buhle E. L., Cohen S., Aebi U. The cell-wall structure of a magnesium-dependent Halobacterium, Halobacterium volcanii CD-2 from the Dead Sea. J. Ultrastruct. Mol. Struct. Res. 100:1988a;94-106.
14. Kessel M., Wildhaber I., Cohen S., Baumeister W. Three-dimensional structure of the regular surface glycoprotein layer of Halobacterium volcanii from the Dead Sea. EMBO J. 7:1988b;1549-1554.
15. Lamvik M. K., Kopf D. A., Davilla S. D. Mass loss rate in collodion is greatly reduced at liquid helium temperature. J. Microsc. 148:1987;211-217.
16. Lamvik M. K., Magid A. D., Davilla S. D., Cordova L. Temperature directly affects the rate of irradiation-induced mass loss from phosphatidylcholine multilayers. Ultramicroscopy. 35:1991;351-356.
17. Lechner J., Sumper M. The primary structure of a procaryotic glycoprotein cloning and sequencing of the cell surface glycoprotein gene of halobacteria. J. Biol. Chem. 262:1987;9724-9729.
18. Luther P. K. Sample shrinkage and radiation damage. Frank J. Electron Tomography: Three-Dimensional Imaging with the Electron Microscope. 1992;39-60 Plenum, New York.
19. Luther P. K., Lawrence M. C., Crowther R. A. A method for monitoring the collapse of plastic sections as a function of electron dose. Ultramicroscopy. 24:1988;7-18.
20. Saxton W. O. Semper: Distortion compensation, selective averaging, 3-D reconstruction, and transfer function correction in a highly programmable system. J. Struct. Biol. 116:1996;230-236.
21. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26:1969;31-43.
22. Sumper M. Primary structure and glycosylation of the S-layer protein of Haloferax volcanii. J. Bacteriol. 172:1990;7111-7118.
23. Trachtenberg S. Fast-freezing devices for cryo-electron-microscopy. Micron. 24:1993a;1-12.
24. Trachtenberg S. Programmable freeze-substitution and cryoembedding device. Microsc. Res. Tech. 24:1993b;173-179.