Comparison of analytical methods for quantitation of isolated porcine hepatocyte yields

Tissue Engineering

Volumn 6, Issue 3, 2000, Pages 253-264

  Stegemann, Jan P ^b   Raina, Santosh ^a   Nicholson, Donald T ^a   Jimenez, Pedro ^a   Shah, Lisa ^a   Cain, Shawn ^a   Chandler, Barbara ^a   Pitkin, Zorina ^a   Mullon, Claudy ^c   Custer, Linda ^d  

^a Circe Biomedical   (United States)

^b GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^c NOVARTIS PHARMA AG   (Switzerland)

^d Sontra Medical   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURACY; ANALYTIC METHOD; ANIMAL CELL; ARTICLE; AUTOANALYSIS; CELL COUNT; CELL ISOLATION; CELL SIZE; CONTROLLED STUDY; CRYOPRESERVATION; INTERMETHOD COMPARISON; LIVER CELL; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; SWINE;

ANIMALS; CELL COUNT; CELL TRANSPLANTATION; LIVER; LIVER TRANSPLANTATION; SWINE;

EID: 0033917609     PISSN: 10763279     EISSN: None     Source Type: Journal    
DOI: 10.1089/10763270050044434     Document Type: Article

References (27)

1. Woolf, G. Definitions and etiology. In: Demetriou, A.A., ed. Support of the Acutely Failing Liver. Austin TX: RG Landes Company, 1994.
2. Eguchi, S., Chen, S.C., Rozga, J., and Demetriou, A.A. Tissue engineering: Liver. In: Lanza, R.P., and Chick, W.L., eds. Yearbook of Cell and Tissue Transplantation 1996/1997. Norwell, MA: Kluwer Academic, 1996, pp. 247-252.
3. Cao, S., Esquivel, C.O., and Keeffe, E.B. New approaches to supporting liver failure. Ann. Rev. Med. 49, 85, 1998.
4. Sussman, N.L., and Kelly, J.H. Extracorporeal liver support: cell-based therapy for the failing liver. Am. J. Kidney Dis. 30, S66, 1997.
5. Watanabe, F.D., Mullon, C.J.P., Hewitt, W.R., Arkadopoulos, N., Kahaku, E., Eguchi, S., Khalili, T., Arnaout, W., Shackleton, C.R., Rozga, J., Solomon, B., and Demetriou, A.A. Clinical experience with a bioartificial liver in the treatment of severe liver failure. Ann. Surg. 225, 484, 1997.
6. Chen, S.C., Mullon, C., Kahaku, E., Watanabe, F., Hewitt, W., Eguchi, S., Middleton, Y., Arkadopoulos, N., Rozga, J., Solomon, B., and Demetriou, A.A. Treatment of severe liver failure with a bioartificial liver. Ann. NY Acad. Sci. 831, 350, 1997.
7. Mullon, C., and Pitkin, Z. The hepatassist bioartificial liver support system: clinical study and pig hepatocyte process. Exp. Opin. Invest. Drugs 8, 229, 1999.
8. Gerlyng, P., Stokke, T., Huitfeldt, H.S., Stenersen, T., Danielsen, H.E., Grotmol, T., and Seglen, P.O. Analytical methods for the study of liver cell proliferation. Cytometry 13, 404, 1992.
9. Hübl, W., Andert, S., Erath, A., Lapin, A., and Bayer, P.M. Peripheral blood monocyte counting : towards a new reference method. Eur. J. Clin. Chem. Clin. Biochem. 33, 839, 1995.
10. Heaney, L.G., McKirgan, J., Stanfor, C.F., and Ennis, M. Electronic cell counting to measure total cell numbers in bronchoalveolar lavage fluid. Eur. Respir. J. 7, 1527, 1994.
11. Horiuti, Y., Ogishima, M., Yano, K., and Shibuya, Y. Quantification of cell nuclei isolated from hepatocytes by cell lysis with nonionic detergent in citric acid. Cell Struct. Funct. 16, 203, 1991.
12. Li, R.C., Lee, S.W., and Lam, J.S. Novel method for assessing postantibiotic effect by using the coulter counter. Antimicrob. Agents Chemother., 40, 1751, 1996.
13. Stegemann, J.P., O'Neil, J.J., Nicholson, D.T., and Mullon, C.J.P. Improved assessment of isolated islet tissue volume using digital image analysis. Cell Transpl. 7, 469, 1998.
14. Souchier, C., Ffrench, M., Benchaib, M., Catallo, R., and Bryon, P.A. Methods for cell proliferation analysis by fluorescent image cytometry. Cytometry 20, 203, 1995.
15. Teague, T.K., Munn, L., Zygourakis, K., and McIntyre, B.W. Analysis of lymphocyte activation and proliferation by video microscopy and digital imaging. Cytometry 14, 772, 1993.
16. Mohler, W.A., Charlton, C.A., and Blau, H.M. Spectrophotometric quantitation of tissue culture cell number in any medium. BioTechniques 21, 260, 1996.
17. Labarca, C., and Paigen, K. A simple, rapid, and sensitive DNA assay procedure. Anal. Biochem. 102, 344, 1980.
18. McCaffrey, T.A., Agarwal, L.A., and Weksler, B.B. A rapid fluorometric DNA assay for the measurement of cell density and proliferation in vitro. In Vitro Cell. Dev. Biol. 24, 247, 1988.
19. Seglen, P.O. Preparation of isolated rat liver cells. Methods Cell Biol. 29, 1976.
20. Morsiani, E., Rozga, J., Scott, H., et al. Automated liver cell processing facilitates large-scale isolation and purification of porcine hepatocytes. ASAIO J. 41, 155, 1995.
21. Cain, S., Naik, S., Deane, D., Borland, K., Chandler, B., Santangini, H., Trenkler, D., Velazquez, N., Solomon, B., Jauregui, H., Mullon, C. Large scale cryopreservation of porcine hepatocytes for the clinical treatment of acute liver failure. Cell Transpl. 5, 19, 1996.
22. Jauregui, H.O., Ng, S.F., Gann, K.L., and Waxman, D.J. Xenobiotic induction of P-450 PB-4 (IIB1) and P-450c (IA1) and associated monooxygenase activities in primary cultures of adult rat hepatocytes. Xenobiotica 21, 1091, 1991.
23. Pan, J., Naik, S., Santangini, H., Trenkler, D., and Jauregui, H. Flow cytometric characterization of isolated porcine hepatocyte suspensions for liver support. Artif. Organs 20, 1173, 1996.
24. Alpini, G., Phillips, J.O., Vroman, B., and Larusso, N.F. Recent advances in the isolation of liver cells. Hepatology 20, 494, 1994.
25. Bhatia, S.N., Toner, M., Foy, B.D., Rotem, A., O'Neil, K.M., Tompkins, R.G., and Yarmush, M.L. Zonal liver cell heterogeneity: effects of oxygen on metabolic functions of hepatocytes. Cell. Eng. 1, 125, 1996.
26. Häussinger, D., and Schliess, F. Cell volume and hepatocellular function. J. Hepatol., 22, 94, 1995.
27. Darr, T.B. and Hubel, A. Freezing characteristics of isolated pig and human hepatocytes. Cell Transpl. 6, 173, 1997.