Overall rotational diffusion and internal mobility in domain II of protein G from Streptococcus determined from 15N relaxation data

Protein Science

Volumn 9, Issue 6, 2000, Pages 1210-1216

  Tillett, Marcus L ^a   Blackledge, Martin J ^b   Derrick, Jeremy P ^c   Lian, Lu Yun ^a,c   Norwood, Timothy J ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^c UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

15N relaxation; Anisotropic; Dynamics; Model free approach; Protein G; Rotational diffusion

Indexed keywords

PROTEIN G;

ALPHA HELIX; ANISOTROPY; ARTICLE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN STRUCTURE; STREPTOCOCCUS;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STREPTOCOCCUS;

EID: 0033917604     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.9.6.1210     Document Type: Article

References (21)

1. Barchi JJ, Grasberger B, Gronenborn AM, Clore GM. 1994. Investigation of the backbone dynamics of the Igg-binding domain of streptococcal protein-G by heteronuclear 2-dimensional H-1-N-15 nuclear-magnetic-resonance spectroseopy. Protien Sci 3:15-21.
2. Blackledge M, Cordier F, Dosset P, Marion D. 1998. Precision and uncertainty in the characterization of anisotropic rotational diffusion by N-15 relaxation. J Am Chem Soc 120:4538-4539.
3. Clore GM, Szabo A, Bax A, Kay LE, Driscoll PC, Gronenborn AM. 1990. Deviations from the simple 2-parameter model-free approach to the interpretation of N-15 nuclear magnetic-relaxation of proteins. J Am Chem Soc 112:4984-4991.
4. Cordier F, Caffrey M, Brutscher B, Cusanovich MA, Marion D, Blackledge M. 1998. Solution structure, rotational diffusion anisotropy and local backbone dynamics of Rhodobacter capsulatus cytochrome c2. J Mol Biol 281:341-361.
5. Farrow NA, Muhandiram R, Singer AU, Pascal SM, Kay CM, Gish G, Shoelson SE, Pawson T, Formankay JD, Kay LE. 1994. Backbone dynamics of a free and a phosphopeptide-complexed Src homology-2 domain studied by N-15 NMR relaxation. Biochemistry 33:5984-6003.
6. Kay LE. 1998. Protein dynamics from NMR. Nat Struct Biol 5:513-517.
7. Kay LE, Torchia DA, Bax A. 1989. Backbone dynamics of proteins as studied by N-15 inverse detected heteronuclear NMR spectroscopy -Application to staphylococcal nuclease. Biochemistry 28:8972-8979.
8. Kelley LA, Gardner SP, Sutcliffe MJ. 1997. An automated approach for defining core atoms and domains in an ensemble of NMR-derived protein structures. Protein Eng 10:737-741.
9. Kordel J, Skelton NJ, Akke M, Palmer AG, Chazin WJ. 1992. Backbone dynamics of calcium-loaded calbindin-D(9k) studied by 2 dimensional proton-detected N-15 NMR-spectroscopy. Biochemistry 31:4856-4866.
10. Krishnan VV. 1997. Determination of oligomeric state of proteins in solution from pulsed-field-gradient self-diffusion coefficient measurements. A comparison of experimental, theoretical, and hard-sphere approximated values. J Magn Reson 124:468-473.
11. Lian LY, Derrick JP, Sutcliffe MJ, Yang JC, Roberts GCK. 1992. Determination of the solution structures of domain-Ii and domain-Iii of protein-G from streptococcus by H-1 nuclear-magnetic-resonance. J Mol Biol 228: 1219-1234.
12. Lian LY, Yang JC, Derrick JP, Sutcliffe MJ, Roberts GCK, Murphy JP, Goward CR, Atkinson T. 1991. Sequential H-1-NMR assignments and secondary structure of an Igg-binding domain from protein-G. Biochemistry 30:5335-5340.
13. Lipari G, Szabo A. 1982. Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 2. Analysis of experimental results. J Am Chem Soc 104:4559-4570.
14. Luginbuhl P, Pervushin KV, Iwai H, Wüthrich K. 1997. Anisotropic molecular rotational diffusion in N-15 spin relaxation studies of protein mobility. Biochemistry 36:7305-7312.
15. Mandel AM, Akke M, Palmer AG. 1995. Backbone dynamics of Escherichia-coli ribonuclease hi-correlations with structure and function in an active cnzyme. J Mol Biol 246:144-163.
16. Palmer AG. 1997. Probing molecular motion by NMR. Curr Opin Struct Biol 7:732-737.
17. Schurr JM, Babcock HP, Fujimoto BS. 1994. A test of the model-free formulas - Effects of anisotropic rotational diffusion and dimerization. J Magn Reson SerB 105:211-224.
18. Tillett ML, Horsfield MA, Lian LY, Norwood TJ. 1999. Protein-ligand interactions measured by 15N-filtered diffusion experiments. J Biomol NMR 13:223-232.
19. Tjandra N, Feller SE, Pastor RW, Bax A. 1995. Rotational diffusion anisotropy of human ubiquitin from N-15 NMR relaxation. J Am Chem Soc 117:12565-12566.
20. Tjandra N, Szabo A, Bax A. 1996. Protein backbone dynamics and N15 chemical shift anisotropy from quantitative measurement of relaxation interfeence effects. J Am Chem Soc 118:6986-1991.
21. Woessner DE. 1962. Nuclear spin relaxation in ellipsoids undergoing rotational Brownian motion. J Chem Phys 37:647-654.