Adherence of isogenic flagellum-negative mutants of Helicobacter pylori and Helicobacter mustelae to human and ferret gastric epithelial cells

Infection and Immunity

Volumn 68, Issue 7, 2000, Pages 4335-4339

  Clyne, Marguerite ^a,b   Ocroinin, Tadhg ^a,b   Suerbaum, Sebastian ^c,d   Josenhans, Christine ^c,e   Drumm, Brendan ^a,b  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b OUR LADY S CHILDREN S HOSPITAL   (Ireland)

^c RUHR UNIVERSITY BOCHUM   (Germany)

^d UNIVERSITY OF WÜRZBURG   (Germany)

^e UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN;

ANIMAL CELL; ARTICLE; BACTERIAL GENE; BACTERIUM ADHERENCE; BACTERIUM MUTANT; CONTROLLED STUDY; FERRET; FLAGELLUM; GENE MUTATION; HELICOBACTER PYLORI; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; STOMACH EPITHELIUM;

ADHESINS, BACTERIAL; ANIMALS; BACTERIAL ADHESION; CELLS, CULTURED; EPITHELIAL CELLS; FERRETS; FLAGELLA; GASTRIC MUCOSA; GENES, BACTERIAL; HELICOBACTER; HELICOBACTER PYLORI; HUMANS; MUTATION;

EID: 0033916226     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.7.4335-4339.2000     Document Type: Article

References (25)

1. Andrutis, K. A., J. G. Fox, D. B. Schauer, R. P. Marini, X. Li, L. Yan, C. Josenhans, and S. Suerbaum. 1997. Infection of the ferret stomach by isogenic flagellar mutant strains of Helicobacter mustelae. Infect. Immun. 65:1962-1966.
2. Andrutis, K. A., J. G. Fox, D. B. Schauer, R. P. Marini, J. C. Murphy, L. Yan, and J. V. Solnick. 1995. Inability of an isogenic urease-negative mutant strain of Helicobacter mustelae to colonize the ferret stomach. Infect. Immun. 63:3722-3725.
3. Clyne, M., and B. Drumm. 1993. Adherence of Helicobacter pylori to primary human gastrointestinal cells. Infect. Immun. 61:4051-4057.
4. Eaton, K. A., C. L. Brooks, D. R. Morgan, and S. Krakowka. 1991. Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnotobiotic piglets. Infect. Immun. 59:2470-2475.
5. Eaton, K. A., and S. Krakowka. 1994. Effect of gastric pH on urease-dependent colonization of gnotobiotic piglets by Helicobacter pylori. Infect. Immun. 62:3604-3607.
6. Eaton, K. A., D. R. Morgan, and S. Krakowka. 1992. Motility as a factor in the colonisation of gnotobiotic piglets by Helicobacter pylori. J. Med. Microbiol. 37:123-127.
7. Eaton, K. A., S. Suerbaum, C. Josenhans, and S. Krakowka. 1996. Colonization of gnotobiotic piglets by Helicobacter pylori deficient in two flagellin genes. Infect. Immun. 64:2445-2448.
8. Fox, J. G., P. Correa, N. S. Taylor, A. Lee, G. Otto, J. C. Murphy, and R. Rose. 1990. Helicobacler mustelae-associated gastritis in ferrets. An animal model of Helicobacler pylori gastritis in humans. Gastroenterology 99:352-361.
9. Fox, J. G., B. M. Edrise, E. B. Cabot, C. Beaucage, J. C. Murphy, and K. S. Prostak. 1986. Campylobacter-like organisms isolated from gastric mucosa of ferrets. Am. J. Vet. Res. 47:236-239.
10. Foynes, S., N. Dorrell, S. J. Ward, Z. Z. Zhang, A. A. McColm, M. Farthing, and B. W. Wren. 1999. Functional analysis of the roles of FliQ and FIhB in flagellar expression in Helicobacter pylori. FEMS Microbiol. Lett. 174:33-39.
11. Geis, G., S. Suerbaum, B. Forsthoff, H. Leying, and W. Opferkuch. 1993. Ultrastructure and biochemical properties of the flagellar sheath of Helicobacter pylori. J. Med. Microbiol. 38:371-377.
12. Grant, C. C. R., M. E. Konkel, W. Cieplak, Jr., and L. S. Tompkins. 1993. Role of flagella in adherence, internalization, and translocation of Campylobacter jejuni in nonpolarized and polarized epithelial cell cultures. Infect. Immun. 61:1764-1771.
13. Jenks, P. W., S. Foynes, S. J. Ward, C. Constantinidou, C. W. Penn, and B. W. Wren. 1997. A flagellar-specific ATPase (FliI) is necessary for flagellar export in Helicobacter pylori. FEMS Microbiol. Lett. 152:205-211.
14. Josenhans, C., A. Labigne, and S. Suerbaum. 1995. Comparative ultrastructural and functional studies of Helicobacter pylori and Helicobacter mustelae flagellin mutants: both flagellin subunits are necessary for full motility in Helicobacter species. J. Bacteriol. 177:3010-3020.
15. Kostrzynska, M., J. D. Betts, J. W. Austin, and T. J. Trust. 1991. Identification, characterization, and spatial localization of two flagellin species in Helicobacter pylori flagella. J. Bacteriol. 173:937-946.
16. Leying, H., S. Suerbaum, G. Geis, and R. Haas. 1992. Cloning and genetic characterisation of a Helicobacter pylori flagellin gene. Mol. Microbiol. 6:2863-2874.
17. Luke, C. J., and C. W. Penn. 1995. Identification of a 29kDa flagellar sheath protein in Helicobacter pylori using a murine monoclonal antibody. Microbiology 141:597-604.
18. McGee, D. J., C. A. May, R. M. Garner, J. M. Himpsl, and H. L. T. Mobley. 1999. Isolation of Helicobacter pylori genes that modulate urease activity. J. Bacteriol. 181:2477-2484.
19. McSweegan, E., and R. I. Walker. 1986. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect. Immun. 53:141-148.
20. O'Rourke, J., A. Lee, and J. G. Fox. 1992. An ultrastructural study of Helicobacter mustelae: evidence of a specific association with gastric mucosa. J. Med. Microbiol. 36:420-427.
21. O'Toole, P. W., M. Kostrzynska, and T. J. Trust. 1994. Non-motile mutants of Helicobacter pylori and Helicobacter mustelae defective in flagellar hook protein. Mol. Microbiol. 14:691-703.
22. Porwollik, S., B. Noonan, and P. W. O'Toole. 1999. Molecular characterization of a flagellar export locus of Helicobacter pylori. Infect. Immun. 67:2060-2070.
23. Schmitz, A., C. Josenhans, and S. Suerbaum. 1997. Cloning and genetic characterization of the Helicobactaer pylori flbA gene which codes for a membrane protein involved in coordinated expression of flagellar genes. J. Bacteriol. 179:987-997.
24. Suerbaum, S., C. Josenhans, and A. Labigne. 1993. Cloning and genetic characterization of the Helicobacter pylori and Helicobacter mustelae flaB flagellin genes and construction of H. pylori flaA- and flaβ-negative mutants by electroporation-mediated allelic exchange. J. Bacteriol. 175:3278-3288.
25. Tsuda, M., M. Karita, M. G. Morshed, K. Okita, and T. Nakazawa. 1994. A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach. Infect. Immun. 62:3586-3589.