Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase

Applied Microbiology and Biotechnology

Volumn 54, Issue 1, 2000, Pages 97-103

  Domingues, L ^a   Onnela, M L ^b   Teixera, J A ^a   Lima, N ^a   Penttila M ^b  

^a UNIVERSITY OF MINHO   (Portugal)

^b VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; BETA GALACTOSIDASE; COPPER; GALACTOSE; GLUCOSE; LACTOSE; RECOMBINANT DNA;

ARTICLE; ASPERGILLUS NIGER; CELL DENSITY; CHEESE; CONTROLLED STUDY; ENZYME ACTIVITY; FLOCCULATION; NONHUMAN; PROTEIN SECRETION; SACCHAROMYCES CEREVISIAE; WHEY;

ASPERGILLUS NIGER; BETA-GALACTOSIDASE; CULTURE MEDIA; PLASMIDS; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

ASPERGILLUS NIGER; CESTODA; SACCHAROMYCES CEREVISIAE;

EID: 0033913018     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530000358     Document Type: Article

References (27)

1. Bailey MJ, Linco M (1990) Production of β-galactosidase by Aspergillus oryzae in submerged bioreactor cultivation. J Biotechnol 16: 57-66
2. Domingues L, Teixeira JA, Lima N (1997) Rapid and sensitive detection of β-galactosidase-producing yeasts by using microtiter plate assay. Biotechnol Tech 6: 399-402
3. Domingues L, Teixeira JA, Lima N (1999) Construction of a flocculent Saccharomyces cerevisiae fermenting lactose. Appl Microbiol Biotechnol 51: 621-626
4. Henderson RCA, Cox BS, Tubb R (1985) The transformation of brewing yeasts with a plasmid containing the gene for copper resistance. Curr Genet 9: 133-138
5. Henry KL, Davis RH, Taylor AL (1990) Continuous recombinant bacterial fermentations utilizing selective flocculation and recycle. Biotechnol Prog 6: 7-12
6. Holmes DS, Quigley M (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114: 193-197
7. Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153: 163-168
8. Jeong YS, Vieth WR (1991) Fermentation of lactose to ethanol with recombinant yeast in an immobilized yeast membrane bioreactor. Biotechnol Bioeng 37: 587-590
9. Kihn JC, Masy CL, Mestdagh MM (1988) Yeast flocculation: competition between nonspecific repulsion and specific bonding in cell adhesion. Can J Microbiol 34: 773-778
10. Kumar V, Ramakrishnan S, Teeri TT, Knowles JKC, Hartley BS (1992) Saccharomyces cerevisisae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate. Biotechnology 10: 82-85
11. Lennox ES (1955) Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1: 190-206
12. Lima N, Teixeria JA, Mota M (1992) Enhancement of metabolic rates of yeast flocculent cells through the use of polymeric additives. Bioprocess Eng 7: 343-348
13. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor
14. Miki BLA, Poon NH, Seligy VL (1982) Repression and induction of flocculation interaction in Saccharomyces cerevisiae. J Bacteriol 150: 890-899
15. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-428
16. Nishihara H, Toraya T (1987) Essential roles of cell surface protein and carbohydrate components in flocculation of a brewer's yeast. Agric Biol Chem 51: 2721-2726
17. Ramakrishnan S, Hartley BS (1993) Fermentation of lactose by yeast cells secreting recombinant fungal lactase. Appl Environ Microbiol 5: 4230-4235
18. Rubio-Teixeira M, Castrillo JI, Adam AC, Ugalde UO, Polaina J (1998) Highly efficient assimilation of lactose by a metabolically engineered strain of Saccharomyces cerevisiae. Yeast 14: 827-837
19. Shiestl RH, Gietz RD (1989) High efficiency transformation of intact cells using single stranded nucleic acids as a carrier. Curr Genet 16: 339-346
20. Sleep D, Belfield GP, Balance DJ, Steven J, Jones S, Evans LR, Moir PD, Goodey AR (1991) Saccharomyces cerevisiae strains that overexpress heterologous proteins. Biotechnology 9: 183-187
21. Soares EV, Teixeria JA, Mota M (1992) Interaction between flocculent and nonflocculent cells of Saccharomyces cerevisiae. Can J Microbiol 38: 969-974
22. Sreekrishna K, Dickson RC (1985) Construction of strains of Saccharomyces cerevisiae that grow on lactose. Proc Natl Acad Sci USA 82: 7909-7913
23. Teixeria JA, Mota M (1992) Flocculation bioreactors. In: Villa TG, Abalde J (eds) Profiles on Biotechnology. Servicio de publicaciones, Universidad de Santiago de Compostela
24. Teixeira JA, Mota M, Goma G (1990) Continuous ethanol production by a flocculating strain of Kluyveromyces marxianus: bioreactor performance. Bioprocess Eng 5: 123-127
25. Teixeria JA, Oliveira R, Azeredo J, Sousa M, Sil C (1995) Cell wall surface properties and flocculence of a Kluyveromyces marxianus strain. Colloids Surf B: Biointerfaces 5: 197-203
26. Watari J, Nomura M, Sahara H, Koshino S (1994) Construction of flocculent brewer's yeast by chromosomal integration of the yeast flocculation gene FLO1. J Inst Brew 100: 73-77
27. Yang S, Shu C (1996) Kinetics and stability of GM-CSF. Production by recombinant yeast cells in immobilized in a fibrousbed bioreactor. Biotechnol Prog 12: 449-456