Demethylating reagent 5-azacytidine inhibits telomerase activity in human prostate cancer cells through transcriptional repression of hTERT

Clinical Cancer Research

Volumn 6, Issue 7, 2000, Pages 2868-2875

  Kitagawa, Yasuhide ^a   Kyo, Satoru ^a   Takakura, Masahiro ^a   Kanaya, Taro ^a   Koshida, Kiyoshi ^a   Namiki, Mikio ^a   Inoue, Masaki ^a  

^a KANAZAWA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AZACITIDINE; MESSENGER RNA; TELOMERASE;

ARTICLE; CARCINOGENESIS; CELL IMMORTALIZATION; ENZYME INHIBITION; ENZYME SUBUNIT; GENE EXPRESSION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; ONCOGENE C MYC; PRIORITY JOURNAL; PROMOTER REGION; PROSTATE CANCER; TRANSCRIPTION REGULATION;

AZACITIDINE; CATALYTIC DOMAIN; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, NEOPLASTIC; GENES, REPORTER; HUMANS; LUCIFERASES; MALE; PROSTATIC NEOPLASMS; RNA; TELOMERASE; TRANSCRIPTION, GENETIC; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0033912372     PISSN: 10780432     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (53)

1. Blackburn, E. H. Structure and function of telomeres. Nature (Lond.), 350: 569-573, 1991.
2. Watson, J. D. Origin of concatameric T7 DNA. Nat. New Biol., 239: 197-201, 1972.
3. Greider, C. W., and Blackburn, E. H. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature (Lond.), 337: 331-337, 1989.
4. Kim, N. W., Piatyszek, M. A., Prowse, K. R., Harley, C. B., West, M. D., Ho, P. L. C., Oviello, G. M., Wright, W. E., Weinrich, S. L., and Shay, J. W. Specific association of human telomerase activity with immortal cells and cancer. Science (Washington DC), 266: 2011-2015, 1994.
5. Hiyama, E., Gollahon, L., Kataoka, T., Kuroi, K., Yokoyama, T., Gazdor, A. F., Hiyama, K., Piatyszek, M. A., and Shay, J. W. Telomerase activity in human breast tumors. J. Natl. Cancer Inst., 88: 116-122, 1996.
6. Kyo, S., Kunimi, K., Uchibayashi, T., Namiki, M., and Inoue, M. Telomerase activity in human urothelial tumors. Am. J. Clin. Pathol., 5: 556-560, 1997.
7. Lin, Y., Uemura, H., Fujinami, K., Hosaka, M., Harada, M., and Kubota, Y. Telomerase activity in primary prostate cancer. J. Urol., 157: 1161-1165, 1997.
8. Counter, C. M., Avilion, A. A., LeFeuvre, C. E., Stewart, N. G., Greider, C. W., Harley, C. B., and Bacchetti, S. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J., 11: 1921-1929, 1992.
9. Counter, C. M., Hirte, H. W., Bacchetti, S., and Harley, C. B. Telomerase activity in human ovarian carcinoma. Proc. Natl. Acad. Sci. USA, 91: 2900-2904, 1994.
10. Kyo, S., Takakura, M., Kohama, T., and Inoue, M. Telomerase activity in human endometrium. Cancer Res., 57: 610-614, 1997.
11. Hiyama, K., Hirai, Y., Kyoizumi, S., Akiyama, M., Hiyama, E., Piatyszek, M. A., Shay, J. W., Isioka, S., and Yamakido, M. Activation of telomerase in human lymphocytes and hematopoietic progenitor cells. J. Immunol., 155: 3711-3715, 1995.
12. Sharma, H. W., Sokoloski, J. A., Perez, J. R., Maltese, J. Y., Sartorelli, A. C., Stein, C. A., Nichols, G., Khaled, Z., Telang, N. T., and Narayanan, R. Differentiation of immortal cells inhibits telomerase activity. Proc. Natl. Acad. Sci. USA, 92: 12343-12346, 1995.
13. Albanell, J., Han, W., Mellado, B., Gunawardane, R., Scher, H. I., Dmitrovsky, E., and Moore, M. A. S. Telomerase activity is repressed during differentiation of maturation-sensitive but not resistant human tumor cell lines. Cancer Res., 56: 1503-1508, 1996.
14. Feng, J., Funk, W. D., Wang, S. S., Weirich, A. A. A., Chiu, C. P., Adams, R. R., Chang, E., Allsopp, R. C., Yu, J., Le, S., West, M. D., Harley, C. B., Andrew, W. H., Greider, C. W., and Villeponteau, B. The RNA component of human telomerase RNAs. Science (Washington DC), 269: 1236-1241, 1995.
15. Harrington, L., McPhail, T., Mar, V., Zhou, W., Oulton, R., Bass, M. B., Arruda, I., and Robinson, M. O. A mammalian telomerase-associated protein. Science (Washington DC), 275: 973-977, 1997.
16. Nakayama, J., Saito, M., Nakamura, H., Matsuura, A., and Ishikawa, F. TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family. Cell, 88: 875-884, 1997.
17. Meyerson, M., Counter, C. M., Eaton, E. N., Ellisen, L. W., Steiner, P., Caddle, S. D., Ziaugra, L., Beijersbergen, R. L., Davidoff, M. J., Liu, Q., Bacchetti, S., Haber, D. A., and Weinberg, R. A. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell, 90: 785-795, 1997.
18. Nakamura, T. M., Morin, G. B., Chapman, K. B., Weinrich, S. L., Andrews, W. H., Lingner, J., Harley, C. B., and Cech, T. R. Telomerase catalytic subunit homologs from fission yeast and human. Science (Washington DC), 277: 955-959, 1997.
19. Takakura, M., Kyo, S., Kanaya, T., Tanaka, M., and Inoue, M. Expression of human telomerase subunits and correlation with telom-erase activity in cervical cancer. Cancer Res., 58: 1558-1561, 1998.
20. Ito, H., Kyo, S., Kanaya, T., Takakura, M., Inoue, M., and Namiki, M. Expression of human telomerase subunits and correlation with telomerase activity in urothelial cancer. Clin. Cancer Res., 4: 1603-1608, 1998.
21. Kanaya, T., Kyo, S., Takakura, M., Ito, H., Namiki, M., and Inoue, M. hTERT is a critical determinant of telomerase activity in renal cell carcinoma. Int. J. Cancer, 78: 539-543, 1998.
22. Weinrich, S. L., Pruzan, R., Ma, L., Ouellette, M., Tesmer, V. M., Holt, S. E., Bodnar, A. G., Lichsterner, S., Kim, N. W., Trager, J. B., Taylor, R. D., Carlos, R., Andrews, W. H., Wright, W. E., Shay, J. W., Harley, C. B., and Morin, G. B. Reconstitution of human telomerase with template RNA component hTERC and the catalytic protein subunit hTERT. Nat. Genet., 17: 498-502, 1997.
23. Nakayama, J., Tahara, H., Tahara, E., Saito, M., Ito, K., Nakamura, H., Nakanishi, T., Tahara, E., Ide, T., and Ishikawa, F. Telomerase activation by hTRT in human normal fibroblasts and hepatocellular carcinomas. Nat. Genet., 18: 65-68, 1998.
24. Takakura, M., Kyo, S., Kanaya, T., Hirano, H., Takeda, J., Yutsudo, M., and Inoue, M. Cloning of human telomerase catalytic subunit (hTERT) gene promoter and identification of proximal core promoter sequences essential for transcriptional activation in immortalized and cancer cells. Cancer Res., 59: 551-557, 1999.
25. Wu, K. J., Grandori, C., Amacker, M., Vermot, N. S., Polack, A., Lingner, J., and Favera, R. D. Direct activation of hTERT transcription by c-myc. Nat. Genet., 21: 220-224, 1999.
26. Greenberg, R. A., O' Hagan, R. C., Deng, H., Xiao, Q., Hann, S. R., Adams, R. R., Lichtsteiner, S., Chin, L., Morin, G. B., and DePinho, R. A. Telomerase reverse transcriptase gene is a direct target of c-Myc but is not functionally equivalent in cellular transformation. Oncogene, 18: 1219-1226, 1999.
27. Jones, P. A., and Taylor, S. M. Cellular differentiation, cytidine analogs and DNA methylation. Cell, 20: 85-93, 1980.
28. Herman, J. G., Latif, F., Weng, Y., Lerman, M. I., Zbar, B., Liu, S., Samid, D., Duan, D-S. R., Gnarra, J. R., Linehan, W. M., and Baylin, S. B. Silencing of the VHL gene by DNA methylation in renal carcinoma. Proc. Natl. Acad. Sci. USA, 91: 9700-9704, 1994.
29. Herman, J. G., Merlo, A., Mao, L., Lapidus, R. G., Issa, J-P., Davidson, N. E., Sidransky, D., and Baylin, S. B. Inactivation of the CDKN2/p16/ MTS1 gene is frequently associated with aberrant DNA methylation in all common human cancers. Cancer Res., 55: 4525-4530, 1995.
30. INK4B1. Cancer Res., 56: 722-727, 1996.
31. Prasanna, P., Thibault, A., Liu, L., Weber, J., and Samid, D. Therapeutic potentials of 5-aza-2′-deoxycytidine on human prostatic cancer cells. Proc. Am. Assoc. Cancer Res., 36: 404, 1995.
32. Thibault, A., Figg, W. D., Bergan, R. C., Lush, R. M., Myers, C. E., Tompkins, A., Reed, E., and Samid, D. A Phase II study of 5-aza-2′-deoxycytidine (decitabine) in hormone independent metastatic (D2) prostate cancer. Tumori, 84: 87-89, 1998.
33. Stone, K. R., Mickey, D. D., Wunderli, H., Mickey, G. H., and Paulson, D. F. Isolation of a human prostate carcinoma cell line (DU-145). Int. J. Cancer, 21: 274-281, 1978.
34. Iizumi, T., Yazaki, T., Kanoh, S., Kondo, I., and Koiso, K. Establishment of a new prostatic carcinoma cell line (TSU-PR1). J. Urol., 137: 1304-1306, 1987.
35. Tatematsu, K., Nakayama, J., Danbara, M., Shionoya, S., Sato, H., Omine, M., and Ishikawa, F. A novel quantitative "stretch PCR assay" that detects a dramatic increase in telomerase activity during the progression of myeloid leukemias. Oncogene, 13: 2265-2274, 1996.
36. Kyo, S., Takakura, M., Tanaka, M., and Inoue, M. Telomerase activity in cervical cancer is quantitatively distinct from that in its precursor lesions. Int. J. Cancer, 79: 66-70, 1998.
37. Serrano, M., Hannon, G. J., and Beach, D. A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Nature (Lond.), 366: 704-707, 1993.
38. Ben-Yosef, T., Yanuka, O., Halle, D., and Benvenisty, N. Involvement of Myc targets in c-myc and N-myc induced human tumors. Oncogene, 17: 165-171, 1998.
39. Ng, S., Gunning, P., Eddy, R., Ponte, P., Leavitt, J., Shows, T., and Kedes, L. Evolution of the functional human β-actin gene and its multipseudogene family: conservation of noncoding regions and chromosomal dispersion of pseudogenes. Mol. Cell. Biol., 5: 2720-2732, 1985.
40. Higuchi, R. Recombinant PCR. In: M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (eds.), PCR Protocols: A Guide to Methods and Applications, pp. 177-183. San Diego, CA: Academic Press, 1990.
41. Takebe, Y., Seiki, M., Fujisawa, J., Hoy, P., Yokota, K., Arai, K., Yoshida, M., and Arai., N. SRα promoter: an efficient and versatile mammalian cDNA expression system composed of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol., 8: 466-472, 1988.
42. Kitaura, H., Galli, I., Taira, T., Iguchi-Ariga, S. M., and Ariga, H. Activation of c-myc promoter by c-myc protein in serum-starved cells. FEBS Lett., 290: 147-152, 1991.
43. Graham, F. L., and Van der Eb, A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology, 52: 452-467, 1973.
44. Schreiber, E., Matthias, P., Muller, M. M., and Schaffer, W. Rapid detection of octamer binding proteins with mini-extract prepared from a small number of cells. Nucleic Acids Res., 17: 6419, 1989.
45. Galaktionov, K., Chen, X., and Beach, D. Cdc25 cell-cycle phosphatase as a target of c-myc. Nature (Lond.), 382: 511-517, 1996.
46. INK4, the product of the putative tumor suppressor gene MTSI. Oncogene, 9: 3475-3482, 1994.
47. Oswald, F., Lovec, H., Möröy, T., and Lipp, M. E2F-dependent regulation of human MYC: trans-activation by cyclins D1 and A overrides tumour supressor protein functions. Oncogene, 9: 2029-2036, 1994.
48. INK4A as upstream regulators of Myc. Oncogene, 15: 179-192, 1997.
49. Faraoni, I., Turriziani, M., Masci, G., De Vecchis, L., Shay, J. W., Bonmassar, E., and Graziani, G. Decline in telomerase activity as a mesure of tumor cell killing by antineoplastic agent in vitro. Clin. Cancer Res., 3: 579-585, 1997.
50. Melana, S. M., Holland, J. F., and Pogo, B. G-T. Inhibition of cell growth and telomerase activity of breast cancer cells in vitro by 3′-azido-3′-deoxythymidine. Clin. Cancer Res., 4: 693-696, 1998.
51. Wang, J., Xie, L. Y., Allan, S., Beach, D., and Hannon, G. J. Myc activates telomerase. Genes Dev., 12: 1769-1774, 1998.
52. Fujimoto, K., and Takahashi, M. Telomerase activity in human leukemic cell lines is inhibited by antisense pentadeoxynucleotides targeted against c-myc mRNA. Biochem. Biophys. Res. Commun., 241: 775-781, 1997.
53. Jarrard, D. F., Kinoshita, H., Shi, Y., Sandefur, C., Hoff, D., Meisner, L. F., Chang, C., Herman, J. G., Isaacs, W. B., and Nassif, N. Methylation of the androgen receptor promoter CpG island is associated with loss of androgen receptor expression in prostate cancer cells. Cancer Res., 58: 5310-5314, 1998.