Comparison of NCCLS and 3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) methods of in vitro susceptibility testing of filamentous fungi and development of a new simplified method

Journal of Clinical Microbiology

Volumn 38, Issue 8, 2000, Pages 2949-2954

  Meletiadis, J ^a   Meis, J F G M ^a   Mouton, J W ^a   Donnelly, J P ^a   Verweij, P E ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ALBACONAZOLE; AMPHOTERICIN B; ANTIFUNGAL AGENT; FORMAZAN; ITRACONAZOLE; MICONAZOLE; TERBINAFINE; UNCLASSIFIED DRUG; VORICONAZOLE;

ARTICLE; ASPERGILLUS; DILUTION; DRUG SENSITIVITY; FUSARIUM; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; SCEDOSPORIUM;

ASPERGILLUS FLAVUS; ASPERGILLUS FUMIGATUS; FUSARIUM OXYSPORUM; FUSARIUM SOLANI; SCEDOSPORIUM APIOSPERMUM; SCEDOSPORIUM PROLIFICANS;

EID: 0033884748     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.38.8.2949-2954.2000     Document Type: Article

References (20)

1. Comparison of a photometric method with standardized methods of antifungal susceptibility testing of yeasts
2. Standardization of antifungal susceptibility testing
3. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability
4. Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi
5. Multicenter evaluation of proposed standardized procedure for antifungal susceptibitity testing of filamentous fungi
6. An improved MTT assay using the electron-coupling agent menadione
7. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill
8. Comparison of a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl ]-2H-tetrazolium hydroxide (XTT) colorimetric method with the standardized National Committee for Clinical Laboratory Standards method of testing clinical yeast isolates for susceptibility to antifungal agents
9. Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay
10. Colorimetric susceptibility testing for Aspergillus fumigatus: Comparison of menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide and Alamar blue tests
11. A rapid colorimetric assay of fungal viability with the tetrazolium salt MTT
12. In vitro interaction of terbinafine with itraconazole against clinical isolates of Scedosporium prolificans
13. A simplified new assay for assessment of fungal cell damage with the tetrazolium dye, (2,3)-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carb oxanilide (XTT)
14. Towards an optimized MTT assay
15. Antifungal susceptibility testing. Current state of technology, limitations, and standardization
16. Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates
17. Comparison study of broth macrodilution and microdilution antifungal susceptibility tests for the filamentous fungi
18. Antifungal susceptibility testing
19. An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT