A new genetic method for isolating functionally interacting genes: High plo1+-dependent mutants and their suppressors define genes in mitotic and septation pathways in fission yeast

Genetics

Volumn 155, Issue 4, 2000, Pages 1521-1534

  Cullen, C Fiona ^a,b   May, Karen M ^a   Hagan, Iain M ^c   Glover, David M ^b,d   Ohkura, Hiroyuki ^a,b  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF DUNDEE   (United Kingdom)

^c UNIVERSITY OF MANCHESTER   (United Kingdom)

^d UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHATIDYLINOSITOL 4 PHOSPHATE KINASE; PHOSPHOTRANSFERASE;

AMINO ACID SEQUENCE; ARTICLE; GENE EXPRESSION; GENE ISOLATION; GENE MAPPING; GENE REPRESSION; MITOSIS; MITOSIS SPINDLE; MOLECULAR INTERACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; YEAST;

AMINO ACID SEQUENCE; CELL CYCLE PROTEINS; CELL DIVISION; DNA-BINDING PROTEINS; DROSOPHILA PROTEINS; GENETIC COMPLEMENTATION TEST; GENETIC TECHNIQUES; GENOTYPE; MITOSIS; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MUTATION; PHENOTYPE; PROTEIN-SERINE-THREONINE KINASES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUPPRESSION, GENETIC; TEMPERATURE; TIME FACTORS;

SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

EID: 0033884342     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (76)

1. The Polo-like kinase Plx1 is a component of the MPF amplification loop at the G2/M-phase transition of the cell cycle in Xenopus eggs
2. Basic local alignment search tool
3. Role of polo kinase and Mid1p in determining the site of cell division in fission yeast
4. A new tropomyosin essential for cytokinesis in the fission yeast S. pombe
5. The Schizosaccharomyces pombe cdc3 gene encodes a profilin essential for cytokinesis
6. Isolation and characterization of new fission yeast cytokinesis mutants
7. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe
8. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
9. Duplication of spindle plaques and integration of the yeast cell cycle
10. Asymmetry of the spindle pole bodies and spg1p GAP segregation during mitosis in fission yeast
11. Isolation and characterization of fission yeast mutants defective in the assembly and placement of the contractile actin ring
12. The Polo-related kinase Cdc5 activates and is destroyed by the mitotic cyclin destruction machinery in S. cerevisiae
13. Cell cycle regulation of the Saccharomyees cerevisiae polo-like kinase cdc5p
14. The small GTP-binding protein Rho regulates a phosphatidylinositol 4-phosphate 5-kinase in mammalian cells
15. Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p
16. The polo-like kinase Plx1 is required for M phase exit and destruction of mitotic regulators in Xenopus egg extracts
17. 'Mass-murder' of ORFs from three regions of chromosome XI from Saccharomyees cerevisiae
18. The Schizosaccharomyces pombe cdc14 gene is required for septum formation and can also inhibit nuclear division
19. The cdc7 protein kinase is a dosage dependent regulator of septum formation in fission yeast
20. The S. pombe cdc16 gene is required both for maintenance of p34(cdc2) kinase activity and regulation of septum formation: A link between mitosis and cytokinesis?
21. The S. pombe cdc15 gene is a key element in the reorganisation of F-actin at mitosis
22. A conserved mitotic kinase active at late anaphase-telophase in syncytial Drosophila embryos
23. Requirement of phosphatidylinositol 4,5-bisphosphate for alpha-actinin function
24. Byr4 and Cdc16 form a two-component GTPase-activating protein for the Spg1 GTPase that controls septation in fission yeast
25. Regions of byr4, a regulator of septation in fission yeast, that bind spg1 or cdc16 and form a two-component GTPase-activating protein with cdc16
26. Regulation of vinculin binding to talin and actin by phosphatidyl-inositol-4-5-bisphosphate
27. Polo kinase: The choreographer of the mitotic stage?
28. Polo-like kinases: A team that plays throughout mitosis
29. 1996: A vintage year for yeast and Yeast
30. Cell cycle analysis and chromosomal localization of human Plk1, a putative homologue of the mitotic kinases Drosophila polo and Saccharomyces cerevisiae Cdc5
31. Cell cycle regulation of the activity and subcellular localization of Plk1, a human protein kinase implicated in mitotic spindle function
32. The role of Myo2, a yeast class V myosin, in vesicular transport
33. A novel role for Cdc5p in DNA replication
34. Isolation and characterisation of Schizosaccharomyces pombe cut mutants that block nuclear division but not cytokinesis
35. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli
36. Modulation of gelsolin function by phosphatidylinositol 4,5-bisphosphate
37. A late mitotic regulatory network controlling cyclin destruction in Saccharomyces cerevisiae
38. The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicals
39. Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae
40. A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5
41. PKA and MPF-activated polo-like kinase regulate anaphase-promoting complex activity and mitosis progression
42. Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts
43. Antibody microinjection reveals an essential role for human polo-like kinase 1 (Plk1) in the functional maturation of mitotic centrosomes
44. Specific interaction between phosphatidylinositol 4,5-bisphosphate and profilactin
45. Polo encodes a protein kinase homolog required for mitosis in Drosophila
46. Genetic interactions in the control of septation in Schizosaccharomyces pombe
47. Thiamine-repressible expression vectors pREP and pRIP for fission yeast
48. + gene encodes a novel EF-hand protein essential for cytokinesis
49. Uncontrolled septation in a cell division cycle mutant of the fission yeast Schizosaccharomyces pombe
50. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe
51. Plo1 kinase recruitment to the spindle pole body and its role in cell division in Schizosaccharomyces pombe
52. The fission yeast dma1 gene is a component of the spindle assembly checkpoint, required to prevent septum formation and premature exit from mitosis if spindle function is compromised
53. The small GTP-binding protein Rho1 is a multifunctional protein that regulates actin localization, cell polarity, and septum formation in the fission yeast Schizosaccharomyces pombe
54. Polo-like kinases: Positive regulators of cell division from start to finish
55. Analysis of a method for selecting temperature-sensitive mutants of BHK cells
56. Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe
57. The conserved Schizosaccharomyces pombe kinase plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells
58. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe
59. Activated polo-like kinase Plx1 is required at multiple points during mitosis in Xenopus laevis
60. Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system
61. Systematic gain-of-function genetics in Drosophila
62. Sce3, a suppressor of the Schizosaccharomyces pombe septation mutant cdc11, encodes a putative RNA-binding protein
63. The Spg1p GTPase is an essential, dosage-dependent inducer of septum formation in Schizosaccharomyces pombe
64. The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase
65. The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae
66. The dmf1/mid1 gene is essential for correct positioning of the division septum in fission yeast
67. Asymmetric segregation on spindle poles of the Schizosaccharomyces pombe septum-inducing protein kinase cdc7p
68. A novel suppressor of ras1 in fission yeast, byr4, is a dosage-dependent inhibitor of cytokinesis
69. Sid2p, a spindle pole body kinase that regulates the onset of cytokinesis
70. CDC5 and CKII control adaptation to the yeast DNA damage check-point
71. Rho GTPase and signaling networks
72. A gene encoding a protein with seven zinc finger domains acts on the sexual differentiation pathways of Schizosaccharomyces pombe
73. Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae
74. gCap39, A calcium ion- and polyphosphoinositide-regulated actin capping protein