Capillary zone electrophoresis with on-line blotting for separation and detection of 32P-postlabelled DNA adducts

Biomarkers

Volumn 5, Issue 4, 2000, Pages 314-321

  Schmitz, Oliver ^a,b   Richter, Elmar ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

32P postlabelling; Capillary electrophoresis; DNA adducts

Indexed keywords

CARCINOGEN; NUCLEOTIDE DERIVATIVE; PHOSPHORUS 32;

ANALYTIC METHOD; ARTICLE; BIOLOGICAL MONITORING; CAPILLARY ZONE ELECTROPHORESIS; CHEMICAL LABELING; DNA ADDUCT; IMMUNOBLOTTING; MALE; NONHUMAN; RAT; THIN LAYER CHROMATOGRAPHY;

EID: 0033880466     PISSN: 1354750X     EISSN: None     Source Type: Journal    
DOI: 10.1080/135475000413863     Document Type: Article

References (22)

1. BARRY, J. P., NORWOOD, C. and VOUROS, P. 1996, Detection and identification of Benzo[a]pyrene diol epoxide adducts to DNA utilizing capillary electrophoresis-electrospray mass spectrometry. Analytical Chemistry, 68, 1432-1438.
2. 32P-postlabeling assay. Carcinogenesis, 13, 1053-1074.
3. CHENG, Y-F., FUCHS, M., ANDREWS, D. and CARSON, W. 1992, Membrane fraction collection for capillary electrophoresis. Journal of Chromatograph, 608, 109-116.
4. DEFORCE, D. L. D., RYNIERS, F. P. K., VAN DEN EECKHOUT, E. G., LEMIERE, F. and ESMANS, E. L. 1996, Analysis of DNA adducts in DNA hydrolysates by capillary zone electrophoresis and capillary zone electrophoresis-electrospray mass spectrometry. Analytical Chemistry, 68, 3575-3584.
5. DEFORCE, D. L. D., LEMIERE, F., HOES, I., MILLECAMPS, R. E. M., ESMANS, E. L., DE LEENHEER, A. and VAN DEN EECKHOUT, E. G. 1998, Analysis of the DNA adducts of phenyl glycidyl ether in a calf thymus DNA hydrolysate by capillary zone electrophoresis-electrospray mass spectrometry: evidence for phosphate alkylation. Carcinogenesis, 19, 1077-1086.
6. DING, J. and VOUROS, P. 1997, Capillary electrochromatography-mass spectrometry for the analytes of DNA adduct mixtures. Analytical Chemistry, 69, 379-384.
7. ERIKSSON, K.-O., PALM, A. and HJERTEN, S. 1992, Preparative capillary electrophoresis based on adsorption of the solutes (proteins) onto a moving blotting membrane as they migrate out of the capillary. Analytical Biochemistry, 201, 211-215.
8. FANG, J.-L. and VACA, C. E. 1997, Detection of DNA adducts of acetaldehyde in perpheral white blood cells of alcohol abusers. Carcinogenesis, 18, 627-632.
9. 32P-postlabeling analysis of non-radioactive aromatic carcinogen-DNA adducts. Carcinogenesis, 3, 1081-1092.
10. HUANG, X. and ZARE:, R. N. 1990, Continuous sample collection in capillary zone electrophoresis by coupling the outlet of a capillary to a moving surface. Journal of Chromatography, 516, 185-189.
11. LEE, T. and YEUNG, E. S. 1991, Micellar electrokinetic capillary chromatographic separation and laser-induced fluorescence detection of 2′-deoxynucleoside 5′-monophosphates of normal and modified bases. Journal of Chromatography, 565, 197-206.
12. MAGNÚSDÓTTIR, S., HELLER, C., SERGOT, P and VIOVY, J. L. 1997, Micropreparative capillary electrophoresis of DNA by direct transfer onto a membrane. Electrophoresis, 18, 1990-1993.
13. 32P-post-labelled DNA adducts by the development of a novel automated on-line solid-phase extraction HPLC method. Carcinogenesis, 19, 1061-1069.
14. NORWOOD, C. B., JACKIM, E. and CHEER, S. 1993, DNA adduct research with capillary electrophoresis. Analytical Biochemistry. 213, 194-199.
15. PENTONEY, S. L. and ZARE, R. N. 1989, Semiconductor radioisotope detector for capillary electrophoresis. Journal of Chromatography, 480, 259-270.
16. PHILLIPS, D. H. and CASTEGNARO, M. 1999, Standardization and validation of DNA adduct postlabelling methods; report of interlaboratory trials and production of recommended protocols. Mutagenesis, 14(3), 301-315.
17. 32P-postlabeled DNA adducts. Journal of Chromatography, 677, 265-273.
18. 32P-base analysis of DNA. Analytical Biochemistry, 117, 271-279.
19. TRACHT, S., TOMA, V. and SWEEDLER, J. V. 1994, Postcolumn radionuclide detection of low-energy beta emitters in capillary electrophoresis. Analytical Chemistry, 66, 2382-2389.
20. TRACHT, S. E., CRUZ, L., STOBBA-WILEY, C. M. and SWEEDLER, J. V. 1996, Detection of radionuclides in capillary electrophoresis using a phosphor-imaging detector. Analytical Chemistry, 68, 3922-3927.
21. WANG, P. and GIESE, R. W. 1998, Phosphate-specific fluorescence labeling with Bo-IMI: reaction details. Journal of Chromatography A, 809, 211-218.
22. WOLF, S. M. and VOUROS, P. 1995, Incorporation of sample stacking techniques into the capillary electrophoresis CF-FAB mass spectrometric analysis of DNA adducts. Analytical Chemistry, 67, 891-900.